创伤弧菌微球口服疫苗的制备及其免疫效果研究

doi:10.19640/j.cnki.jtau.2023.06.008

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (6): 41-50.doi: 10.19640/j.cnki.jtau.2023.06.008

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Investigation on the immune effect of Vibrio vulnificus inactivated vaccine in fish

He Xuexin, Wu Zun, Wu Yating, Gao Haofeng, Zhou Wenli, Shao Peng^{Corresponding Author}

College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-11-16 Online:2023-12-31 Published:2024-02-05

Abstract

Abstract: In order to study the immune effect of inactivated Vibrio vulnificus vaccine, this study prepared Vibrio vulnificus microsphere vaccine by inactivating the Vibrio vulnificus strain EPL0201 stored in the laboratory with 0.3% formaldehyde and wrapping the inactivated bacteria solution with sodium alginate. Healthy pearl gentian grouper was immunized by oral route, and was injected intraperitoneally with 1×10⁷ cfu/mL Vibrio vulnificus to challenge the pearl gentian grouper. The expression trend of some immune related genes of the pearl gentian grouper after immunization and the immune protection rate after challenge were detected. The results showed that the particle size of Vibrio vulnificus microsphere oral vaccine was（34.55±5.33）μm. The coverage rate was 90.5%. Its safety was good. Quantitative real-time PCR（QRT-PCR）results showed that, compared with the control group, each immune gene had different expression changes successively. They participated in immune response in kidney, spleen, liver and intestine which involved the genes TLR1, TLR2, TLR3, TLR5, and Myd88 in the TLR signaling pathway and inflammatory cytokines IL-1β, IL-12P40 and TNF-α. The expression of immune genes in different tissues was different, and the highest expression level of IL-1β was in spleen, and the highest value was 72.37 times that of the control group. The peak of peak expression of the same immune gene in different organs and tissues of fish was different, and the peak expression of different immune genes in the same tissue was also different, which indicated that the gene expression changes in different tissues were different. The results of challenge experiment showed that the immune protection rate was 61.3%. The results of this study showed that Vibrio vulnificus oral vaccine can induce immune genes to participate in the immune response, and has immune protection effect on pearl Gentian grouper. It also provides experimental data support for the prevention and treatment of diseases caused by Vibrio vulnificus with microsphere oral vaccine.

Key words: Vibrio vulnificus, oral microsphere vaccine, pearl gentian grouper, immune effect

CLC Number:

S942.5

He Xuexin, Wu Zun, Wu Yating, Gao Haofeng, Zhou Wenli, Shao Peng. Investigation on the immune effect of Vibrio vulnificus inactivated vaccine in fish[J]. Journal of Tianjin Agricultural University, 2023, 30(6): 41-50.

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http://xuebao.tjau.edu.cn/EN/Y2023/V30/I6/41

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Investigation on the immune effect of Vibrio vulnificus inactivated vaccine in fish

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