响应面法优化雨生红球藻产虾青素培养条件

doi:10.19640/j.cnki.jtau.2019.03.013

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (3): 57-61.doi: 10.19640/j.cnki.jtau.2019.03.013

• Researches and Scientific Notes • Previous Articles Next Articles

Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology

LI Jia-yi, DOU Yong, SHAO Peng, GAO Jin-wei, JIA Xu-ying, ZHANG Wen-hui, WANG Yi-zhe, MA Ting, ZHOU Wen-li

Tianjin Fisheries Resources and Environment Research Office, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-02-27 Online:2019-09-30 Published:2019-09-30

Abstract

Abstract: Haematococcus pluvialis is an important resource microalgae. The product astaxanthin has excellent antioxidant activity and is widely used in medicine and food. In order to study the high-yield astaxanthin of Haematococcus pluvialis, three stress conditions（5 000, 7 000, 9 000 lx）, sodium nitrate concentrations（0, 0.13, 0.25 g/L）and chlorination were studied. The effects of sodium concentrations（0, 2.5, 5.0 g/L）on astaxanthin content of Haematococcus pluvialis were optimized by response surface methodology. The order of influence of the three factors on the production of astaxanthin content was: sodium chloride concentration>light intensity>sodium nitrate concentration. The optimal extraction conditions were: light intensity 9 000 lx, sodium nitrate concentration 0 g/L, sodium chloride concentration 2.5 g/L, under which the astaxanthin content could reach 6.8 mg/L, and it was similar to the predicted value. The results indicated that the model has high reliability.

Key words: Haematococcus pluvialis, astaxanthin, light intensity, response surface

CLC Number:

DF413.4

LI Jia-yi, DOU Yong, SHAO Peng, GAO Jin-wei, JIA Xu-ying, ZHANG Wen-hui, WANG Yi-zhe, MA Ting, ZHOU Wen-li. Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 57-61.

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Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology

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