两种饵料和投喂频率对三疣梭子蟹生长、消化和免疫指标的影响

doi:10.19640/j.cnki.jtau.2021.04.010

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (4): 41-46.doi: 10.19640/j.cnki.jtau.2021.04.010

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of two kinds of diets and feeding frequency on growth, digestion and immune response of Portunus trituberculatus

Zhou Jing¹, Huang Yadong², Zhou Xuan¹, Yang Jiayue¹, Qiao Xiuting¹, Cheng Zhenyan^{1,Corresponding Author}

1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Haisheng Aquaculture Co., Ltd., Tianjin 300270, China

Received:2021-04-08 Online:2021-12-31 Published:2022-01-05

Abstract

Abstract: A 28 d feeding trial was conducted to study the effects of different diets and feeding frequency on the growth, digestion and immune indexes of Portunus trituberculatus. A two-factor experimental design was adopted with Aloididae aloidis and black soldier fly larvae as baits and the feeding frequency set as twice/d and 3 times/d, respectively. 36 crabs with an initial weight of （75.8±16.8） g were randomly divided into 4 groups with 3 replicates in each group and 3 crabs in each replicate. The results showed that feeding frequency and diet type did not significantly affect the weight gain rate and specific growth rate of Portunus trituberculatus. Feeding black soldier fly significantly increased the hepatopancreas index（P<0.05）. The protease and cellulase activity of crab were significantly influenced by feeding frequency and diet type（P<0.05）, and the feeding frequency also affected the amylase activity（P<0.05）. The feeding frequency and diet type did not influence the acid phosphatase（ACP）and total superoxide dismutase（T-SOD）activity of crab, but showed a significant effect on the activity of alkaline phosphatase（AKP）（P<0.05）. Also, the diet type showed a significant effect on the Catalase（CAT）activity, which was higher in crab fed with black soldier fly than that fed with aloididae aloidis（P<0.05）. In conclusion, feeding frequency did not significantly affect the growth performance of Portunus trituberculatus. There was no significant effect on the growth performance of Portunus trituberculatus no matter when fed with black soldier fly larvae or Aloididae aloidis. However, the non-specific immunity of crab fed with black soldier fly larvae improved. Generally, it was possible in practice to feed Portunus trituberculatus with Black soldier fly larvae instead of Aloididae aloidis.

Key words: Portunus trituberculatus, black soldier fly, growth performance, digestive enzymes, non-specific immunity

CLC Number:

S963.21

Zhou Jing, Huang Yadong, Zhou Xuan, Yang Jiayue, Qiao Xiuting, Cheng Zhenyan. Effects of two kinds of diets and feeding frequency on growth, digestion and immune response of Portunus trituberculatus[J]. Journal of Tianjin Agricultural University, 2021, 28(4): 41-46.

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Effects of two kinds of diets and feeding frequency on growth, digestion and immune response of Portunus trituberculatus

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