高效转基因水稻连续种植对土壤微生物功能多样性的影响

doi:10.19640/j.cnki.jtau.2019.02.001

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (2): 1-7.doi: 10.19640/j.cnki.jtau.2019.02.001

• Researches and Scientific Notes • Next Articles

Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity

CHEN Jing-yi^1,2, LIU Rui-hua^1,2, WANG Li-li², LI Jing^{2, 3}, LI Gang², YANG Dian-lin², ZHAO Jian-ning²

1. College of Agronomy and Resource Enviroment, Tianjin Agricultural University, Tianjin 300384, China;
2. Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Rural Affairs,Tianjin 300191, China;
3. College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China

Received:2018-08-24 Revised:2017-01-02 Online:2019-06-20 Published:2019-09-19

Abstract

Abstract: In order to evaluate the effects of phosphorus-efficient transgenic rice cultivation on soil microbial functional diversity, the plot experiment was conducted with phosphorus-efficient transgenic rice as a target, and with both phosphorus and non-phosphorus treatments , to analyze and compare the effects of phosphorus-efficient transgenic rice and non transgenic rice on soil microbial functional diversity at different growth stages based on the Biolog eco-plate method. The results showed that the average absorbance value AWCD of soil microflora in OsPT4 and Nipponbare had the same change trend, which all increased with the prolongation of culture time, but the maximum value and rate of change of AWCD in different growth stages were different. The results of soil microbial diversity index analysis showed that the phosphorus-efficient transgenic OsPT4 and Nipponbare Shannon's richness index and Simpson dominance index showed higher in P application than no P application during the whole growth period; Shannon index of OsPT4 with high P transgenic rice in the tillering and jointing stages, the differences were significant, and the heading and flowering stages were not significantly different from the maturation stage. The Simpson index showed no significant difference in the first three growth stages, but showed a significant difference in the mature stage. McIntosh's index of phosphorus efficient transgenic rice OsPT4 and Nipponbare was the highest in the tillering period, and the value was much higher than the other three growth periods; the McIntosh index of the phosphorus-efficient transgenic OsPT4 rice had no significant difference in the whole growth period. The use of different types of carbon sources to display phosphorus-efficient transgenic rice OsPT4 and Nipponbare are mainly by sugars, carboxylic acids, and amino acids. The principal component analysis showed that Nipponbare was clustered and the phosphorus-efficient transgenic rice OsPT4 was distributed, indicating that there was a difference in the metabolic use of the soil microbial community carbon sources. In summary, compared with the non-transgenic rice, the phosphorus-efficient rice OsPT4 will affect the soil microbial community richness index, dominance index, and the distribution of carbon source principal components due to different phosphorus application conditions and different growth periods.

Key words: rice, phosphorus-efficient, transgenic, soil microbial, Biolog eco-plate method

CLC Number:

S511
S154.3

CHEN Jing-yi, LIU Rui-hua, WANG Li-li, LI Jing, LI Gang, YANG Dian-lin, ZHAO Jian-ning. Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity[J]. Journal of Tianjin Agricultural University, 2019, 26(2): 1-7.

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Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity

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