天津农学院学报 ›› 2022, Vol. 29 ›› Issue (3): 19-22.doi: 10.19640/j.cnki.jtau.2022.03.004

• 研究与简报 • 上一篇    下一篇

盐胁迫下水杨酸对苜蓿幼苗生理特性的影响

王丹a, 王璐瑶b, 董家僖b, 田秀平b,通信作者   

  1. 天津农学院,a.园艺园林学院,b.农学与资源环境学院,天津 300392
  • 收稿日期:2021-05-09 出版日期:2022-09-30 发布日期:2022-10-10
  • 通讯作者: 田秀平(1965—),女,教授,博士,从事土壤和植物营养学的教学与科研工作。E-mail:tian5918@sohu.com。
  • 作者简介:王丹(1979—),女,副教授,硕士,从事植物学教学及相关科学研究工作。E-mail:wd724@163.com。
  • 基金资助:
    天津市种业科技重大专项(16ZXZYNC00150); 国家绿色肥料产业技术体系项目(CARS-22)

Effects of salicylic acid on physiological characteristics of alfalfa seedlings under salt stress

Wang Dana, Wang Luyaob, Dong Jiaxib, Tian Xiupingb,Corresponding Author   

  1. Tianjin Agricultural University, a. College of Horticulture and Landscape, b. College of Agronomy and Resource Environment, Tianjin 300392, China
  • Received:2021-05-09 Online:2022-09-30 Published:2022-10-10

摘要: 为了解外源物对盐胁迫下苜蓿幼苗生理特性的影响,以苜蓿品种‘中苜3号’为试验材料,用1 mmol/L水杨酸溶液浸种12 h,分析了苜蓿幼苗在不同含盐量下的可溶性蛋白、可溶性糖、脯氨酸和丙二醛的含量,以及过氧化物酶和超氧化物歧化酶活性。结果表明:在土壤含盐量为0.20%~0.52%范围内,水杨酸显著提高了苜蓿幼苗的可溶性蛋白含量、可溶性糖含量、超氧化物歧化酶活性和过氧化物酶活性;在土壤含盐量为0.35%~0.52%范围内,水杨酸显著提高了苜蓿幼苗的脯氨酸含量;在土壤含盐量为0.20%~0.52%范围内,水杨酸显著降低了苜蓿幼苗的丙二醛含量。外源水杨酸通过增强苜蓿幼苗的抗氧化酶活性、增加渗透物质含量和降低丙二醛含量等生理特性以提高其抗盐能力。

关键词: 苜蓿, 盐胁迫, 水杨酸, 幼苗, 生理特性

Abstract: In order to understand the effects of exogenous salicylic acid on physiological characteristics of alfalfa seedlings under salt stress, the contents of soluble protein, soluble sugar, proline and malondialdehyde, as well as the activities of peroxidase and superoxide dismutase were analyzed by soaking the alfalfa variety ‘Zhongmu No. 3’ in 1 mmol/L salicylic acid solution for 12 h and 5 kinds of salt contents. The results showed that the soluble protein content, soluble sugar content, superoxide dismutase activity and peroxidase activity of alfalfa seedlings were significantly increased compared with the control group by salicylic acid in the range of soil salt content from 0.20% to 0.52%. The proline content of alfalfa seedlings was significantly increased with salicylic acid at soil salt content from 0.35% to 0.52%. The content of malondialdehyde in alfalfa seedlings was significantly reduced by salicylic acid when the soil salt content was 0.20%~0.52%. The salt resistance of alfalfa seedlings could be enhanced with exogenous salicylic acid by enhancing the activity of antioxidant enzymes, increasing the content of osmotic substances and decreasing the content of malondialdehyde.

Key words: alfalfa, salt stress, salicylic acid, seedlings, physiological characteristics

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