关键词: 茎尖快繁, 激素, 基质, 扦插, 移栽

Abstract: In order to propagate tower poplar (Populusⅹcanense) rapidly, the technologies of tissue culture, tube culture seedlings transplanting, cutting and cultivation were researched by hormonal combination, culture material combination, seedling hardening, seedling cultivation in the nutritive cups etc., and the factory breeding process for the tissue culture of Tower Poplar and the main technologies were summarized. The results show that more and better buds were gotten by young tip stem culture in the bud differentiation substrate of MS+0.5 mg/L 6-BA+0.5 mg/L IAA. The roots were produced in all plants by all concentrations of IAA and NAA in the test. But, the root quality and quantity were quite different. IAA treatment induced more roots compared with NAA. More and better roots were produced by the culture in the root induction substrate of 1/2 MS+0.25 mg/L IAA. The transplanting survival rate of seedlings was 92% on vermiculite, but only 30% on peat. The dense roots were grown in nutrition pots after seedlings had been cultivated in vermiculite for one month. The cutting propagation and cultivation management was relative to the selection of cutting, the hormone of rooting, and the culture materials and so on.

Key words: rapid propagation of tip stem, hormone, medium, cutting, transplant