猪胰脂肪酶在麸皮上的固定化及催化活性

doi:10.19640/j.cnki.jtau.2024.06.012

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (6): 75-78.doi: 10.19640/j.cnki.jtau.2024.06.012

猪胰脂肪酶在麸皮上的固定化及催化活性

陈璐^a, 丛方地^{a,通讯作者}, 马丽亚^a, 王英超^a, 张达娟^b, 冯自燃^a

天津农学院,a.基础科学学院; b.水产学院天津市水产生态与养殖重点实验室,天津 300392

收稿日期:2024-02-26 出版日期:2024-12-31 发布日期:2024-12-31
通讯作者: 丛方地（1968—）,男,教授,博士,主要从事生物催化方面研究。E-mail：congfangdi@163.com。
作者简介:陈璐（2001—）,女,本科在读,主要从事生物合成方面研究。E-mail：203337157@qq.com。
基金资助:
天津市自然科学基金项目（19JCYBJC30000）; 天津市水产生态及养殖重点实验室开放基金项目（TJAE201802）

Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity

Chen Lu^a, Cong Fangdi^{a,Corresponding Author}, Ma Liya^a, Wang Yingchao^a, Zhang Dajuan^b, Feng Ziran^a

Tianjin Agricultural University, a. College of Basic Sciences, b. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 300392, China

Received:2024-02-26 Online:2024-12-31 Published:2024-12-31

摘要/Abstract

摘要： 为提高猪胰脂肪酶的催化作用,采用物理吸附的方法,在柱形玻璃瓶中,在37 ℃、160 r/min条件下,将固定在小麦麸皮上,将其用于在37 ℃、160 r/min条件下,催化正己醇与乙酸乙烯酯发生非水转酯反应及花生油水解反应。结果发现,当麸皮与酶的质量比为20:10（mg:mg）时,固定化酶的非水催化活性最高,相对于10 mg的酶粉,活性提高10倍以上。在循环催化转酯反应过程中,在第5次使用之后,固定化酶催化底物反应的转化率降低不足0.2%,显示固定化酶具有较好的催化稳定性。在催化花生油水解的过程中,固定化酶水解花生油的能力相当于酶粉的4.7倍,显示其有用作饲料添加剂的潜力,以辅助动物消化。

关键词: 脂肪酶, 催化作用, 活性, 固定化, 麸皮

Abstract: To enhance the catalytic ability of porcine pancreas lipase, it was immobilized on wheat bran by physical adsorption in a column glass bottle at 37 ℃ and 160 r/min, and used to catalyze the non-aqueous transesterification between hexanol and vinyl acetate as well as the hydrolysis of peanut oil at 37 ℃ and 160 r/min. It was found that the suitable mass ratio of wheat bran and lipase was determined to be 20:10（mg:mg）. At this ratio, the immobilized lipase behaved the highest non-aqueous activity, being more than10-fold activity of 10 mg native lipase. The activity decreased less than 0.2% after the immobilized lipase was reused 5 times in the organic system, each time for 8 h, which showed that it had a good catalytic stability. In hydrolysis of peanut oil, the immobilized lipase could hydrolyze peanut oil 4.7 folds of that accomplished by the native lipase with equal mass, which means that it has potential as a feed additive to assist animal digestion.

Key words: lipase, catalysis, activity, immobilization, wheat bran

中图分类号:

Q814

陈璐, 丛方地, 马丽亚, 王英超, 张达娟, 冯自燃. 猪胰脂肪酶在麸皮上的固定化及催化活性[J]. 天津农学院学报, 2024, 31(6): 75-78.

Chen Lu, Cong Fangdi, Ma Liya, Wang Yingchao, Zhang Dajuan, Feng Ziran. Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity[J]. Journal of Tianjin Agricultural University, 2024, 31(6): 75-78.

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http://xuebao.tjau.edu.cn/CN/Y2024/V31/I6/75

参考文献

[1] PUTRA S S S,BASIRUN W J,HAYYAN A. Enzymatic hydrolysis for the removal of 3-monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose[J]. Biochemical Engineering Journal,2023,196:1-11.
[2] ZHAO J,MA M,YAN X,et al.Green synthesis of polydopamine functionalized magnetic mesoporous biochar for lipase immobilization and its application in interesterification for novel structured lipids production[J]. Food Chemistry,2022,379(15):1-10.
[3] 路雪纯,辛嘉英,张帅,等. 脂肪酶固定化及其在食品领域中应用的研究进展[J]. 食品工业科技,2021,42(17):423-431.
[4] GIRELLI A M,CHIAPPINI V.Renewable,sustainable,and natural lignocellulosic carriers for lipase immobilization:A review[J]. Journal of Biotechnology,2023,365:29-47.
[5] 王文雯,胡静,周胜杰,等. 小头鲔幼鱼不同消化器官中消化酶活性分析[J]. 天津农学院学报,2023,30(4):27-31.
[6] 王琳. 几种脂肪酶的固定化及其应用研究[D]. 福州:福州大学,2018.
[7] 周亚梅,刘佳,陆丹,等. 脂肪酶的固定化及其在药物合成中的应用进展[J]. 生物技术进展,2023,13(2):220-227.
[8] PINTO M C C,SOUSA I,DUTRA L,et al. Polymerization strategies to produce new polymer biocatalysts for the biodiesel industry[J]. Journal of Applied Polymer Science,2022,139(11):1-19.
[9] ZHANG S,SHANG W,YANG X,et al.Immobilization of lipase using alginate hydrogel beads and enzymatic evaluation in hydrolysis of p-nitrophenol butyrate[J]. Bulletin of the Korean Chemical Society,2013,34(9):2741-2746.
[10] YAO L W,KHAN F S A,MUBARAK N M,et al. Insight into immobilization efficiency of lipase enzyme as a biocatalyst on the graphene oxide for adsorption of azo dyes from industrial wastewater effluent[J]. Journal of Molecular Liquids,2022,354:1-5.
[11] YUAN M,CONG F,ZHAI Y,et al.Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate[J]. Bioprocess and Biosystems Engineering,2022,45:453-464.
[12] CAO W,CONG F,KANG J,et al.A simple room temperature-static bioreactor for effective synthesis of hexyl acetate[J]. Green Processing and Synthesis,2020,9:48-55.
[13] HE T,ZHANG X,ZHAO L,et al.Insoluble dietary fiber from wheat bran retards starch digestion by reducing the activity of alpha-amylase[J]. Food Chemistry,2023,426(15):1-8.
[14] GAO S,WANG Y,WANG T,et al.Immobilization of lipase on methyl-modified silica aerogels by physical adsorption[J]. Bioresource Technology,2009,99(2):996-999.
[15] 任德忠,丛方地,王晓红,等. PCL生物反应器中催化合成乙酸正戊酯[J]. 天津农学院学报,2020,27(1):65-69.
[16] SANTOS K P D,RIOS N S,LABUS K,et al. Co- immobilization of lipase and laccase on agarose-based supports via layer-by-layer strategy: effect of diffusional limitations[J]. Biochemical Engineering Journal,2022,108533:1-10.
[17] INGENBOSCH K N,VIEYTO-NUNEZ J C,RUIZ- BLANCO Y B,et al. Effect of organic solvents on the structure and activity of a minimal lipase[J]. The Journal of Organic Chemistry,2022,87(3):1669-1678.
[19] 张珍珍,郑达文,宁东,等. 脂肪酶对油脂体外降解及消化的研究[J]. 中国油脂,2020,45(3):140-144.
[19] THAIWONG N,THAIUDOM S.Stability of oil-in-water emulsion influenced by the interaction of modified tapioca starch and milk protein[J]. International Journal of Dairy Technology,2021,74(2):307-315.
[20] ARUMUGAM A,KARUPPASAMY G,JEGADEESAN G B.Synthesis of mesoporous materials from bamboo leaf ash and catalytic properties of immobilized lipase for hydrolysis of rubber seed oil[J]. Materials Letters,2018,225(15):113-116.

猪胰脂肪酶在麸皮上的固定化及催化活性

Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity

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