天津农学院学报 ›› 2016, Vol. 23 ›› Issue (4): 5-8.

• 研究与简报 • 上一篇    下一篇

狨猴腹泻重要病原菌的分离鉴定与药敏试验研究

张占恒1, 2, 高珂珂3, 李小慧3, 林静3, 洪莹莹3, 金天明3, 韩文瑜1,通信作者   

  1. 1. 吉林大学 动物医学学院,长春 130062;
    2. 天津医科大学 实验动物科学部,天津300070;
    3. 天津农学院 动物科学与动物医学学院,天津 300384
  • 收稿日期:2016-08-10 出版日期:2016-12-20 发布日期:2019-10-14
  • 通讯作者: 韩文瑜(1955-),男,河北阜城人,教授,博士,主要从事病原微生物检测与耐药性的研究。E-mail:hanwy@jlu.edu.cn。
  • 作者简介:张占恒(1988-),男,天津市人,实验师,硕士,主要从事病原微生物检测与耐药性的研究。E-mail:zhangzhanheng@tmu.edu.cn。
  • 基金资助:
    天津市优秀科研创新团队资助项目“兽医生物技术”(TD12-5019); 天津市优秀科技特派员项目“规模化猪场腹泻病的监测及净化技术研究”(16JCTPJC45000); 天津市农业科技成果转化与推广项目“规模化猪场仔猪腹泻病的监测及防控技术推广”(201601380)

Study on Isolation and Identification of Marmoset Diarrhoea Pathogenic Bacteria Strain and Its Sensitivity Tests to Drug

ZHANG Zhan-heng1, 2, GAO Ke-ke3, LI Xiao-hui3, LIN Jing3, HONG Ying-ying3, JIN Tian-ming3, HAN Wen-yu1, Corresponding Author   

  1. 1. College of Veterinary Medicine, Jilin University, Changchun 130062, China;
    2. Department of Laboratory Animal, Tianjin Medical University, Tianjin 300070, China;
    3. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
  • Received:2016-08-10 Online:2016-12-20 Published:2019-10-14

摘要: 狨猴腹泻病是一种常见疾病,临床症状主要表现为腹泻、消瘦、精神不振等。对临床发现的天津市某动物中心腹泻狨猴,采集粪便进行细菌分离培养、显微镜镜检、生化试验并进行16S rRNA片段扩增等,鉴定出导致该猴群腹泻的病原菌主要是大肠杆菌和沙门菌。药敏试验显示大肠杆菌对庆大霉素高度敏感,沙门菌对庆大霉素和氨苄西林高度敏感,为该病的治疗提供了依据。

关键词: 狨猴, 腹泻, 病原菌, 分离鉴定, 药敏试验

Abstract: Diarrhoea is a kind of marmoset disease which mainly caused diarrhea, weight loss and spirit depressed. In this paper, the stool samples were collected from marmoset in an animal center in Tianjin. Then the samples were used for a series of laboratory diagnosis, such as bacterial isolation and culture, microscopic examination, biochemical tests and PCR detection of 16s rRNA to identified the isolated strains. The results showed that the isolated strains were Escherichia coli and Salmonella. Drug sensitive test was examined, and the results showed that the isolated Escherichia coli strain is highly sensitive to gentamicin, the isolated Salmonella strain is highly sensitive to gentamicin and ampicillin.

Key words: marmoset, diarrhoea, isolation and identification, drugs sensitive test

中图分类号: