李斯特菌噬菌体裂解酶的研究进展

天津农学院学报 ›› 2016, Vol. 23 ›› Issue (1): 62-65.

李斯特菌噬菌体裂解酶的研究进展

刘珊娜, 范志华, 孙溪, 付荣霞, 吴海清

天津农学院食品科学与生物工程学院,天津市农副产品深加工技术工程中心,天津 300384

收稿日期:2015-08-18 出版日期:2016-03-20 发布日期:2019-10-14
作者简介:刘珊娜（1984-）,女,天津市人,讲师,博士,主要从事食品微生物研究。E-mail：shannaliu@tjau.edu.cn。
基金资助:
天津市科技支撑计划项目“基于噬菌体蛋白的李斯特菌快速检测技术及试纸的研发”（14ZCZDNC00003）

Research Progress in Listeria Phage Endolysins

LIU Shan-na, FAN Zhi-hua, SUN Xi, FU Rong-xia, WU Hai-qing

Tianjin Engineering and Technology Research Center of Agricultural Products Processing, College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China

Received:2015-08-18 Online:2016-03-20 Published:2019-10-14

摘要/Abstract

摘要： 单核细胞增生李斯特菌是重要的食源性致病菌,可通过污染的食品进入人体,引发食品安全事件。噬菌体作为李斯特菌生物防治的有效手段,能够特异性识别菌体细胞,造成细菌裂解死亡。噬菌体发挥抑菌活性的关键在于其双链DNA所编码的裂解酶（肽聚糖水解酶类）能够在增殖过程的后期有效裂解宿主菌的细胞壁,将子代噬菌体释放到胞外环境中。本文综述了李斯特菌噬菌体裂解酶的种类、作用原理和结构特征,并探讨了裂解酶的应用领域和未来发展方向。

关键词: 李斯特菌, 噬菌体, 裂解酶, 研究进展

Abstract: Listeria monocytogenes is an important genus of food-borne pathogens. It may transmit into human body through contaminated food and cause food safety issue. Bacteriophages are effective means for Listeria bio-control, which can specifically recognize bacterial cells and lead to bacterial lysis. The key to antibacterial activities of bacteriophages relies on double stranded DNA-encoded endolysins, the murein enzymes which degrade host cell wall and result in release of phage progeny to the extracellular environment at the end of the phage lytic multiplication cycle. This article reviews the category of Listeria phage endolysins, the mechanism and structural feature of them, and discusses the application area and direction for development of endolysins in the future.

Key words: Listeria, bacteriophage, endolysin, research progress

中图分类号:

S852.61

刘珊娜, 范志华, 孙溪, 付荣霞, 吴海清. 李斯特菌噬菌体裂解酶的研究进展[J]. 天津农学院学报, 2016, 23(1): 62-65.

LIU Shan-na, FAN Zhi-hua, SUN Xi, FU Rong-xia, WU Hai-qing. Research Progress in Listeria Phage Endolysins[J]. Journal of Tianjin Agricultural University, 2016, 23(1): 62-65.

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http://xuebao.tjau.edu.cn/CN/Y2016/V23/I1/62

参考文献

[1] Kuenne C,Billion A,Mraheil M A,et al.Reassessment of the Listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome[J]. BMC Genomics,2013,14:47.
[2] Winkelströter L K,De Martinis E C P. Effect of bacteriocins and conditions that mimic food and digestive tract on biofilm formation,in vitro invasion of eukaryotic cells and internalin gene expression by Listeria monocytogenes[J]. Probiotics & Antimicro Prot,2013,5(3):153-164.
[3] 徐冬旸. 食源性单核细胞增生李斯特菌对消毒剂及镉协同耐受机制研究[D]. 广州:华南理工大学,2013.
[4] Gandhi M,Chikindas M L.Listeria:A foodborne pathogen that knows how to suvive[J]. Int J Food Microbiol,2007,113(1):1-15.
[5] Mahony J,McAuliffe O,Ross R P,et al. Bacteriophage as biocontrol agents of food pathogens[J]. Curr Opin Biotechnol,2011,22(2):157-163.
[6] Coffey B,Mills S,Coffey A,et al.Phage and their lysins as biocontrol agents for food safety application[J]. Annu Rev Food Sci Technol,2010,1(1):449-468.
[7] O’Flaherty S,Ross R P,Coffey A. Bacteriophage and their lysins for elimination of infectious bacteria[J]. FEMS Microbiol Rev,2009,33(4):801-819.
[8] Borysowski J,Weber-Dabrowska B,Gorski A.Bacteriophage endolysins as a novel class of antibacterial agents[J]. Exp Biol Med (Maywood),2006,231(4):366-377.
[9] Hermoso J A,Garcia J L,Garcia P.Taking aim on bacterial pathogens:from phage therapy to enzybiotics[J]. Curr Opin Microbiol,2007,10(5):461-472.
[10] Arachchi G J G,Cridge A G,Dias-Wanigasekera B M,et al. Effectiveness of phages in the decontamination of Listeria monocytogenes adhered to clean stainless steel,stainless steel coated with fish protein,and as a biofilm[J]. J Ind Microbiol Biotechnol,2013,40(10):1105-1116.
[11] Oliveira J,Castilho F,Cunha A,et al.Bacteriophage therapy as a bacterial control strategy in aquaculture[J]. Aquacult Int,2012,20(5):879-910.
[12] Schultz E W.Listerella Infections:A review[J]. Stanford Medical Bulletin,1945,3:135-151.
[13] Klumpp J,Loessner M J.Listeria phages:Genomes,evolution,and application[J]. Bacteriophage,2013,3(3):e26861.
[14] Carlton R M,Noordman W H,Biswas B,et al.Bacteriophage P100 for control of Listeria monocytogenes in foods:genome sequence,bioinformatic analyses,oral toxicity study,and application[J]. Regul Toxicol Pharmacol,2005,43(3):301-312.
[15] Bren L.Bacteria-eating virus approved as food additive[J]. FDA Consum,2007,41(1):20-22.
[16] Loessner M J.Bacteriophage endolysins-current state of research and applications[J]. Curr Opin Microbiol,2005,8(4):480-487.
[17] Young R Y.Bacteriophage holins:deadly diversity[J]. J Mol Microbiol Biotechnol,2002,4(1):21-36.
[18] Loessner M J,Wendlinger G,Scherer S.Heterogeneous endolysins in Listeria monocytogenes bacteriophages:A new class of enzymes and evidence for conserved holin genes within the siphoviral lysis cassettes[J]. Mol Microbiol,1995,16(6):1231-1241.
[19] Eugster M R,Haug M C,Huwiler S G,et al.The cell wall binding domain of Listeria bacteriophage endolysin PlyP35 recognizes terminal GlcNAc residues in cell wall teichoic acid[J]. Mol Microbiol,2011,81(6):1419-1432.
[20] Eugster M R,Loessner M J.Wall teichoic acid restrict access of bacteriophage endolysin Ply118,Ply511,and PlyP40 cell wall binding domains to the Listeria monocytogenes peptidoglycan[J]. J Bacteriol,2012,194(23):6498-6506.
[21] Loessner M J,Kramer K,Ebel F,et al.C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates[J]. Mol Microbiol,2002,44(2):335-349.
[22] Schmelcher M,Waldherr F,Loessner M J,et al.Listeria bacteriophage peptidoglycan hydrolases feature high thermoresistance and reveal increased activity after divalent metal cation substitution[J]. Appl Microbiol Biotechnol,2012,93(2):633-643.
[23] Schmelcher M,Shabarova T,Eugster M R,et al.Rapid multiplex detection and differentiation of Listeria cells by use of fluorescent phage endolysin cell wall binding domains[J]. Appl Environ Microbiol,2010,76(17):5745-5756.
[24] Gaeng S,Scherer S,Neve H,et al.Gene cloning and expression and secretion of Listeria monocytogenes bacteriophage-lytic enzymes in Lactococcus lactis[J]. Appl Environ Microbiol,2000,66(7):2951-2958.
[25] Kretzer J W,Lehmann R,Schmelcher M,et al.Use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells[J]. Appl Environ Microbiol,2007,73(6):1992-2000.
[26] Walcher G,Stessl B,Wagner M,et al.Evaluation of paramagnetic beads coated with recombinant Listeria phage endolysin-derived cell-wall-binding domain proteins for separation of Listeria monocytogenes from raw milk in combination with culture-based and real-time polymerase chain reaction-based quantification[J]. Foodborne Pathog Dis,2010,7(9):1019-1024.