高效转基因水稻连续种植对土壤微生物功能多样性的影响

doi:10.19640/j.cnki.jtau.2019.02.001

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (2): 1-7.doi: 10.19640/j.cnki.jtau.2019.02.001

• 研究与简报 • 下一篇

高效转基因水稻连续种植对土壤微生物功能多样性的影响

陈静怡^1,2, 刘瑞华^1,2, 王丽丽², 李静^2,3, 李刚², 杨殿林², 赵建宁^{2,通信作者}

1. 天津农学院农学与资源环境学院,天津 300384;
2. 农业农村部环境保护科研监测所,天津 300191;
3. 东北农业大学资源与环境学院,哈尔滨 150030

收稿日期:2018-08-24 修回日期:2017-01-02 出版日期:2019-06-20 发布日期:2019-09-19
通讯作者: 赵建宁（1981-）,男,副研究员,博士,研究方向为转基因作物环境安全评价。E-mail：zhaojn2008@163.com。
作者简介:陈静怡（1990-）,女,硕士在读,研究方向为转基因作物环境安全评价研究。E-mail：chenjy3923@163.com。
基金资助:
国家科技重大专项（2016ZX08012005-005）

Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity

CHEN Jing-yi^1,2, LIU Rui-hua^1,2, WANG Li-li², LI Jing^{2, 3}, LI Gang², YANG Dian-lin², ZHAO Jian-ning²

1. College of Agronomy and Resource Enviroment, Tianjin Agricultural University, Tianjin 300384, China;
2. Key Laboratory of Original Agro-Environment Quality of Ministry of Agriculture and Rural Affairs,Tianjin 300191, China;
3. College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China

Received:2018-08-24 Revised:2017-01-02 Online:2019-06-20 Published:2019-09-19

摘要/Abstract

摘要： 为评价磷高效转基因水稻对土壤微生物功能多样性的影响,以磷高效转基因水稻OsPT4为研究对象,设施磷和不施磷两种处理,基于Biolog生态平板法,分析比较了4个生育时期的OsPT4及其亲本‘日本晴’对土壤微生物功能多样性的影响。结果显示：OsPT4和‘日本晴’的土壤微生物群落平均吸光值AWCD均随培养时间的延长而增加,但不同生育时期AWCD最大值和生长趋势有所不同。土壤微生物多样性指数分析结果显示,OsPT4和‘日本晴’的Shannon多样性指数和Simpson优势度指数在全生育时期均表现为施磷处理高于不施磷处理;OsPT4的Shannon指数在分蘖期和拔节期差异显著,抽穗扬花期和成熟期则差异不显著,Simpson指数则表现为前3个生育时期差异不显著,而在成熟期出现显著差异;OsPT4和‘日本晴’的McIntosh均匀度指数均在分蘖期最大,且远高于其他3个生育时期;OsPT4的McIntosh指数在全生育时期均无显著差异。不同类型碳源利用显示,OsPT4和‘日本晴’均以糖类、羧酸类和氨基酸类为主要碳源。主成分分析显示,‘日本晴’呈聚集分布,OsPT4呈分散分布,表明二者在土壤微生物群落碳源的代谢利用上存在差异。综上所述,OsPT4与非转基因水稻相比,不同施磷条件和不同生育时期对土壤微生物群落活性、多样性指数、优势度指数和碳源主成分分布有所影响。

关键词: 水稻, 磷高效, 转基因, 土壤微生物, Biolog生态平板法

Abstract: In order to evaluate the effects of phosphorus-efficient transgenic rice cultivation on soil microbial functional diversity, the plot experiment was conducted with phosphorus-efficient transgenic rice as a target, and with both phosphorus and non-phosphorus treatments , to analyze and compare the effects of phosphorus-efficient transgenic rice and non transgenic rice on soil microbial functional diversity at different growth stages based on the Biolog eco-plate method. The results showed that the average absorbance value AWCD of soil microflora in OsPT4 and Nipponbare had the same change trend, which all increased with the prolongation of culture time, but the maximum value and rate of change of AWCD in different growth stages were different. The results of soil microbial diversity index analysis showed that the phosphorus-efficient transgenic OsPT4 and Nipponbare Shannon's richness index and Simpson dominance index showed higher in P application than no P application during the whole growth period; Shannon index of OsPT4 with high P transgenic rice in the tillering and jointing stages, the differences were significant, and the heading and flowering stages were not significantly different from the maturation stage. The Simpson index showed no significant difference in the first three growth stages, but showed a significant difference in the mature stage. McIntosh's index of phosphorus efficient transgenic rice OsPT4 and Nipponbare was the highest in the tillering period, and the value was much higher than the other three growth periods; the McIntosh index of the phosphorus-efficient transgenic OsPT4 rice had no significant difference in the whole growth period. The use of different types of carbon sources to display phosphorus-efficient transgenic rice OsPT4 and Nipponbare are mainly by sugars, carboxylic acids, and amino acids. The principal component analysis showed that Nipponbare was clustered and the phosphorus-efficient transgenic rice OsPT4 was distributed, indicating that there was a difference in the metabolic use of the soil microbial community carbon sources. In summary, compared with the non-transgenic rice, the phosphorus-efficient rice OsPT4 will affect the soil microbial community richness index, dominance index, and the distribution of carbon source principal components due to different phosphorus application conditions and different growth periods.

Key words: rice, phosphorus-efficient, transgenic, soil microbial, Biolog eco-plate method

中图分类号:

S511
S154.3

陈静怡, 刘瑞华, 王丽丽, 李静, 李刚, 杨殿林, 赵建宁. 高效转基因水稻连续种植对土壤微生物功能多样性的影响[J]. 天津农学院学报, 2019, 26(2): 1-7.

CHEN Jing-yi, LIU Rui-hua, WANG Li-li, LI Jing, LI Gang, YANG Dian-lin, ZHAO Jian-ning. Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity[J]. Journal of Tianjin Agricultural University, 2019, 26(2): 1-7.

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高效转基因水稻连续种植对土壤微生物功能多样性的影响

Effects of continuous planting of phosphorus-efficient transgenic rice on soil microbial functional diversity

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