天津农学院学报 ›› 2019, Vol. 26 ›› Issue (1): 41-44.doi: 10.19640/j.cnki.jtau.2019.01.010

• 研究与简报 • 上一篇    下一篇

犬源弗氏柠檬酸杆菌的分离与鉴定

张希墨, 陆璐, 刘燕霏通信作者, 杨建德通信作者   

  1. 天津农学院 动物科学与动物医学学院,天津 300384
  • 收稿日期:2018-05-27 出版日期:2019-03-20 发布日期:2019-10-09
  • 通讯作者: 刘燕霏(1970-),女,副教授,硕士,主要从事预防兽医学方面的教学与研究。E-mail:674383573@qq.com。
    杨建德(1969-),男,教授,博士,主要从事预防兽医学方面的教学与研究。E-mail:jiandeyang@126.com。
  • 作者简介:张希墨(1997-),女,本科在读,主要从事预防兽医学方面研究。E-mail:1084959981@qq.com。
  • 基金资助:
    天津农学院大学生创新创业项目(201710061193); 天津农学院附属动物医院委托项目(ZH004903); 天津自然科学项目(17JCYBJC16000)

Isolation and identification of Citrobacter freundii from dogs

ZHANG Xi-mo, LU Lu, LIU Yan-feiCorresponding Author, YANG Jian-deCorresponding Author   

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2018-05-27 Online:2019-03-20 Published:2019-10-09

摘要: 为了阐明影响伴侣性动物化脓性疾患的病原,本研究从学院附属动物医院无菌采集1份患子宫蓄脓犬的子宫分泌物进行病原分离与鉴定。通过菌落形态观察及革兰氏染色、生化试验和

Abstract: In order to clarify the associated pathogens of companion animal suppurative diseases, uterine secretions from a patient pyometra dog at animal hospital affiliated to the college were collected sterilely for pathogen isolation and identification. The isolate was identified as Citrobacter freundii by bacterial isolation and culture, colony morphology observation and Gram’s staining observation, biochemical test and 16S rRNA gene analysis. Antibiotic susceptibility results showed that the isolated bacterium is resistant to Maddie and ampicillin, moderately sensitive to aztreonam, cefazolin, and cefoxitin, highly sensitive to cefoperazone, ceftriaxone, cefepime and other antibiotics, providing reference for prevention and related diseases control of companion animals.

Key words: dog, pyometra, Citrobacter freundii, isolation and identification, drug sensitive test

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