Abstract: Type 2 diabetes is acommon chronic disease, and it is associated with insulin resistance. Loss or dysfunction of β-arrestin2 results in the development of insulin resistance and progression of type 2 diabetes. However, administration of β-arrestin2 significantly amend glucose intolerance and insulin resistance. In this article, We fused SUMO and β-arrestin2 gene with recombinant PCR methods, and then inserted it into pET22b vector, and constructed a expression vector pET22b-SUMO- β-arrestin2 successfully. A recombinant expression plasmid was expressed in Rosetta Blue（DE3）line of E.coli with IPTG at the temperature of 20 ℃. The SUMO-β-arrestin2 fusion protein was purified with Ni-NTA successfully. Lay the foundation for subsequent pharmacodynamic research of β-arrestin2.

Key words: β-arrestin2, diabetic, expression, purify