小麦TaYODA 2D的克隆、表达分析及亚细胞定位

doi:10.19640/j.cnki.jtau.2026.05.001

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (5): 1-8.doi: 10.19640/j.cnki.jtau.2026.05.001

Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum

Chang Siyu, Wen Yuzhe, Cao Gaoyi, Chen Xiaoqiang, Xie Xiaodong^{Corresponding Author}, Bao Shuguang^{Corresponding Author}

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2026-05-27 Published:2026-11-02

Abstract

Abstract: YODA encodes a mitogen-activated protein kinase kinase kinase (MAPKKK), which plays a crucial regulatory role in plant growth and development. To investigate the biological function of YODA in wheat, this study cloned the TaYODA 2D gene from wheat leaves. The gene coding region was 2 670 bp in length, encoding 889 amino acids, and produced a hydrophilic, unstable basic protein. The secondary structure was primarily composed of random coils, α-helices, extended chains, and β-sheets. In addition, the TaYODA 2D protein contained 115 phosphorylation sites and 176 glycosylation sites. The promoter region contained cis-acting elements essential for transcriptional regulation, hormone response, light response, stress induction, as well as metabolic and tissue regulation. Systematic evolutionary analysis revealed that the wheat TaYODA 2D protein shared a close phylogenetic relationship with Brachypodium distachyon. qRT-PCR results demonstrated that the TaYODA 2D gene was expressed in roots, coleoptile, stems, leaves, and spikelets. Subcellular localization analysis indicated that its encoded protein was likely localized to the plasma membrane and cytoplasm. This study provides a theoretical foundation and genetic resources for elucidating the function of the TaYODA 2D gene in wheat and its application in breeding.

Key words: wheat, TaYODA 2D, gene cloning, expression analysis, subcellular localization

CLC Number:

S512.1

Chang Siyu, Wen Yuzhe, Cao Gaoyi, Chen Xiaoqiang, Xie Xiaodong, Bao Shuguang. Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 1-8.

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Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum

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