一株好氧反硝化细菌的分离鉴定及反硝化能力初测

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (2): 44-48.

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Identification and Denitrification Ability of an Aerobic Denitrifier

ZHAN Hai^1,2, WANG Guang-jun^{1,Corresponding Author}, CHEN Cheng-xun²

1. Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-04-25 Online:2017-06-30 Published:2019-10-15

Abstract

Abstract: 17 denitrification strains were isolated from mandarin fish pond water of Sanshui city using BTB （bromothymol blue） spread-plate technique, through the preliminary screening for denitrification strains and determination ability, one aerobic denitrifying bacteria which has strong determination, was named 8F-3. The results showed that the total content of nitrogen was reduced from 100.00 mg/L to 6.51 mg/L by 8F-3 strain in 24 hours and the rate of denitrification was 93.49%; Ammonia nitrogen was reduced from 50.000 mg/L to 1.966 mg/L, the removal rate was 96.07%, Nitrate nitrogen was reduced from 50.00 mg/L to 3.51 mg/L, the removal rate was 92.98%; Nitrite nitrogen was reduced from 0.096 mg/L to 0.071 mg/L, the removal rate was 25.79%. According to the blast results of 8F-3 strain 16S rRNA sequence （Bootstrap 1 000 times）and physiological test for 8F-3 using API method, the bacteria was finally identified as Acinetobacter sp.

Key words: aerobic denitrifier, denitrification ability, 16S rRNA, Acinetobacter

CLC Number:

S917.1

ZHAN Hai, WANG Guang-jun, CHEN Cheng-xun. Identification and Denitrification Ability of an Aerobic Denitrifier[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 44-48.

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Identification and Denitrification Ability of an Aerobic Denitrifier

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