Abstract: The study was conducted to understand the effect of Forsythia suspensa, Coptidis rhizoma, Andrographis paniculata on the blood antioxidant function in Cynoglossus semilaevis. A factorial experiment was designed to evaluate the blood parameters（the total superoxide dismutase（T-SOD）, total antioxidant capacity（T-AOC）, catalase（CAT）, malonaldehyde（MDA）, triglyceride（TG）, total cholesterol（T-CHO））in C. semilaevis after 7 days, 14 days and 21 days. Fish were fed diets containing different doses of F. suspensa, C. rhizoma, A. paniculata（0, 5, 10, 15 g/kg）. The results, diet supplemented with 5 g/kg F. suspensa resulted in significantly highest T-SOD activity after 14 days, diet supplemented with 5 g/kg of F. suspensa resulted in significantly highest CAT activity 21 days, diet supplemented with 15 g/kg F. suspensa resulted in significantly lower MDA content than the control group after 14 days. Diet supplemented with 15 g/kg C. rhizoma resulted in significantly highest T-SOD activity after 14 days. Diet supplemented with 15 g/kg of C. rhizoma resulted in significantly higher T-AOC activity than the control group after 21 days. Diet supplemented with 10 g/kg A. paniculata resulted in significantly highest T-SOD activity, CAT activity and T-AOC activity after 14 days. Diet supplemented with 5 g/kg A. paniculata resulted in significantly lower MDA content than the control group after 21 days. Diet supplemented with 10 g/kg A. paniculata resulted in significantly higher T-CHO content and TG content than the control group after 7 days. The results indicated that F. suspensa, C. rhizoma, A. paniculata added individually had different degrees of influences on blood antioxidant parameters in C. semilaevis.

Key words: Forsythia suspense, Coptidis rhizoma, Andrographis paniculata, Cynoglossus semilaevis, antioxidant parameters