油菜籽中硫苷提取的影响因素研究

Journal of Tianjin Agricultural University ›› 2016, Vol. 23 ›› Issue (2): 41-43.

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Study on Factors of Glucosinolates Extraction from Rapeseed

SHI Ping, BU Lu-xia^{1a,Corresponding Author}, XU Yuan-yuan, WEI Zhen

1. Tianjin Agricultural University,
a. College of Basic Science,
b. Chemical Experiment Teaching Center, Tianjin 300384, China

Received:2015-09-23 Online:2016-06-20 Published:2019-10-14

Abstract

Abstract: The content of glucosinolates extracted from rapeseed was studied by solvent method and single factor experiment. The effects of different parameters on the extraction of glucosinolates, such as solvent concentration, ratio of solid to liquid, temperature, time and extract times, were studied, respectively. By using the visible spectrophotometer, glucosinolates content was measured and the optimum extraction conditions were confirmed. The results indicated that the highest content of glucosinolates with 36.84 µmol/g can be obtained at 70 ℃ for extraction of 6 h by using 70% ethanol as solvent and material to solvent ratio of 1:12. Under the same conditions, the residue amount of glucosinolates was 0.23 µmol/g after two extractions which can be ignored.

Key words: glucosinolates, rapeseed, extraction, visible spectrophotometry

CLC Number:

Q946.9
TS229

SHI Ping, BU Lu-xia, XU Yuan-yuan, WEI Zhen. Study on Factors of Glucosinolates Extraction from Rapeseed[J]. Journal of Tianjin Agricultural University, 2016, 23(2): 41-43.

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Study on Factors of Glucosinolates Extraction from Rapeseed

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