新疆野苹果（Malus sieversii）内生菌的分离与鉴定

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (4): 1-5.

• Orginal Article • Next Articles

Isolation and Identification of Endophytic Fungi in Malus sieversii

LI Fang, ZOU Yan, LIU Bin, ZHANG Yun-xiu, LONG Hong, YAN Guo-rong^*

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2015-05-21 Published:2015-12-31

Abstract

Abstract: The endophytic fungi was isolated from M. sieversii stem using tissue separation method to obtain pure strains endophytes, the endophytic fungi species was identified using the molecular biology method, and the fungi ITS sequence was amplified and the comparation and analyses were made. The results show that 11 strains of endogenous fungi were identified from M. sieversii, which belonged to 3 species of endophytes in two genera（Alternaria sp. and Fusarium sp.）, and there are same kind of endophytes among jujube, palm trees, M. sieversii and sugar cane. Pure strains can be obtained in short time initiated with MS medium and separated by PDA method. This method is simpler and more effective compared with multiple purified isolation method of PDA. These can provide experimental data for inhibiting the growth of endophytes generated from M. sieversii by the tissue culture.

Key words: Malus sieversii, endophytic fungi, tissue separation method, ITS

CLC Number:

S661.1

LI Fang, ZOU Yan, LIU Bin, ZHANG Yun-xiu, LONG Hong, YAN Guo-rong. Isolation and Identification of Endophytic Fungi in Malus sieversii[J]. Journal of Tianjin Agricultural University, 2015, 22(4): 1-5.

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Isolation and Identification of Endophytic Fungi in Malus sieversii

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