新疆野苹果多倍体诱导及叶片特征分析

doi:10.19640/j.cnki.jtau.2021.04.002

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (4): 6-10.doi: 10.19640/j.cnki.jtau.2021.04.002

• Researches and Scientific Notes • Previous Articles Next Articles

Induction of polyploid Malus sieversii（Ledeb.）Roem. and characteristic analysis of its leaves

Zhang Chenghui, Li Kaiming, Zhao Qi, Yu Jie, Gongga Yixi, Hu Baoquan^{Corresponding Author}

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-07-08 Online:2021-12-31 Published:2022-01-05

Abstract

Abstract: Malus sieversii is a rich natural resource and rootstock of apple tree. Mining its utilization method is beneficial to the further development of apple breeding. In this study, tetraploid Malus sieversii was induced by soaking stem apex of seedlings with different concentrations of colchicine, and changes in the characteristic structure of the leaves in tetraploid Malus sieversii was analyzed. The results showed that 0.2% colchicine treatment for 1 day achieved better induction effect. The leaves thickness and chlorophyll content of tetraploid increased, but stomatal density decreased, compared with diploid. Changes in anatomical structure of tetraploid leaves significantly resulted the increase of the cell tense ratio and the decrease of spongy ratio, which were was the leaf characteristics with high resistance. The results can provide a new way for the development of Malus sieversii germplasm resource, new reference and theoretical support for the cultivation of new high-quality apple rootstock and polyploid breeding work of apple.

Key words: Malus sieversii（Ledeb.）Roem., tetraploid, colchicine, leaves, anatomical structure

CLC Number:

Zhang Chenghui, Li Kaiming, Zhao Qi, Yu Jie, Gongga Yixi, Hu Baoquan. Induction of polyploid Malus sieversii（Ledeb.）Roem. and characteristic analysis of its leaves[J]. Journal of Tianjin Agricultural University, 2021, 28(4): 6-10.

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Induction of polyploid Malus sieversii（Ledeb.）Roem. and characteristic analysis of its leaves

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