特异性靶基因在弧菌PCR快速检测中的应用

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (3): 49-53.

• Orginal Article • Previous Articles Next Articles

Application of Specific Target Genes to PCR Rapid Detection for Vibrios

SHAO Peng¹, WANG Xiao-yu², XU Xiao-li¹

1. Tianjin Fishery Technical Extension Station, Tianjin 300221, China;
2. Bohai Fisheries Research Institute of Tianjin, Tianjin 300457, China

Received:2015-03-14 Published:2015-09-30

Abstract

Abstract: Quick and accurate diagnosis of the types of Vibrio can effectively prevent and control the occurrence of Vibrio disease. It is usually needed to use the target genes as the foundation when diagnosing the Vibrio disease of aquatic animals by PCR technology. In recent years, the common target genes for bacterial diagnosis mainly included some house-keeping genes, such as 16S rDNA, 16S-23S intergenic spacer regions, gyrB gene, dnaJ gene and so on, and some specific virulence genes of Vibrio, such as toxR gene, tdh gene, trh gene, vvhA gene, ctxA gene, et al.

Key words: target gene, Vibrio, PCR, rapid detection

CLC Number:

SHAO Peng, WANG Xiao-yu, XU Xiao-li. Application of Specific Target Genes to PCR Rapid Detection for Vibrios[J]. Journal of Tianjin Agricultural University, 2015, 22(3): 49-53.

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Application of Specific Target Genes to PCR Rapid Detection for Vibrios

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