Abstract: A rapid and direct detection method of polycyclic aromatic hydrocarbons（PAHs）in soil using fluorescence spectrometry was proposed. Taking phenanthrene as the research object, the feasibility of direct detection of phenanthrene in soil was verified by fluorescence spectroscopy. Soil samples with different concentrations of phenanthrene were configured and fluorescence spectra of all samples were collected by using a LS-55 fluorescence spectrophotometer produced by Perkin Elmer. Through the analysis of two-dimensional correlation fluorescence spectra, it was determined that the fluorescence peaks at 409 and 430 nm were both from phenanthrene in the soil, and the fluorescence peak at 485 nm was from the soil itself. Standard curves for quantitative analysis of phenanthrene content in soil were established based on the fluorescence intensities at 409 and 430 nm, respectively. The corresponding correlation coefficients R² were 0.98 and 0.99, respectively. Seven samples were selected to establish standard curves with corresponding average errors of 1.69% and 6.71% for three unknown samples, respectively. The results showed that the direct detection of PAH contaminants in soil was feasible based on fluorescence spectrometry. This study is important for realizing real-time, in situ fluorescence detection of PAHs contaminants in soil.

Key words: fluorescence spectroscopy, polycyclic aromatic hydrocarbons, soil, phenanthrene, rapid detection