菩提树组织培养技术研究

Journal of Tianjin Agricultural University ›› 2012, Vol. 19 ›› Issue (2): 30-33.

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Tissue Culture of Ficus religiosa

WANG Ya-zhi¹, SUN Ning^{1, Corresponding Author}, ZHANG Lei, CHEN Xiao-qiang¹, XU Yan-ping²

1. Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China;
2. Tanjin Binhai New Ecological Agriculture Development CO., LTD, Tianjin 300480, China

Received:2012-05-08 Online:2012-06-20 Published:2019-11-12

Abstract

Abstract: Selecting the stem segment with buds as explants, the tissue culture technologies of Ficus religiosa were studied in the experiment. The results show that the optimum doused time was 8 to 10 min in 0.1% HgCl₂, MS+NAA 0.1 mg/L+6-BA 1.0 mg/L was the best medium for inducing axillary buds, the proliferation coefficient was 4.5 on the medium of MS+NAA 0.3 mg/L+6-BA 1.0~2.0 mg/L, the suitable medium for rooting was 1/2 MS+NAA 0.5 mg/L, and the rate of rooting was 92.5%. The higher survival rate could be obtained, with the roots of contaminative plantlets being immersed in sterilized pharmaceutics before transplantation.

Key words: Ficus religiosa, stem segment, tissue culture

CLC Number:

WANG Ya-zhi, SUN Ning, ZHANG Lei, CHEN Xiao-qiang, XU Yan-ping. Tissue Culture of Ficus religiosa[J]. Journal of Tianjin Agricultural University, 2012, 19(2): 30-33.

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Tissue Culture of Ficus religiosa

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