基于改进QuEChERS-UPLC-MS/MS测定鸡蛋和鸡组织中胺鲜酯残留

doi:10.19640/j.cnki.jtau.2026.03.007

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (3): 36-41.doi: 10.19640/j.cnki.jtau.2026.03.007

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Determination of DA-6 residues in eggs and chicken tissues based on improved QuEChERS-UPLC-MS/MS

Jin Xiang¹, Li Cun¹, Li Na², Zhang Yuting², Guo Yongze^{2,Corresponding Author}

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Institute of Agro-product Safety and Nutrition, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China

Received:2024-04-21 Online:2026-06-30 Published:2026-06-30

Abstract

Abstract: Based on the optimized QuEChERS pretreatment technique, an ultra performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）method was developed for the determination of DA-6 residue in eggs and chicken tissues. With DA-6 as the research object, the spiking test was carried out in six matrices, namely, egg, chicken meat, chicken gizzard, chicken blood, chicken liver and chicken heart. The results showed that the linearity of DA-6 in the six matrices was good in the concentration range of 0.000 3-0.030 0 mg/L with the correlation coefficients greater than 0.999. The recoveries of DA-6 in eggs, chicken meat and gizzards at the spiked concentrations of 0.005 mg/kg, 0.010 mg/kg, 0.200 mg/kg ranged from 74.1% to 108.2% with the relative standard deviations（RSD）less than 5.0%. The recoveries of DA-6 in chicken blood, chicken heart and chicken liver at the spiked concentrations of 0.008 mg/kg, 0.050 mg/kg and 0.200 mg/kg were in the range of 70.2%-94.2% with the relative standard deviations（RSD）less than 3.3%. In conclusion, this method for the determination of DA-6 residues in eggs and chicken tissues is simple to operate and meets the requirements of the guidelines for pesticide residue testing in terms of accuracy and precision.

Key words: DA-6, QuEChERS, ultra performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）, chicken, residue

CLC Number:

O657.63

Jin Xiang, Li Cun, Li Na, Zhang Yuting, Guo Yongze. Determination of DA-6 residues in eggs and chicken tissues based on improved QuEChERS-UPLC-MS/MS[J]. Journal of Tianjin Agricultural University, 2026, 33(3): 36-41.

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References

[1] ZHANG H,XIE L,XU P,et al.Dissipation of the plant growth regulator hexanoic acid 2-(diethylamino)ethyl ester in pakchoi and soil[J]. International Journal of Environmental and Analytical Chemistry,2008,88(8):561-569.
[2] 钱翔洲. 胺鲜酯与壳聚糖互作对花生光合生理特性、产量及品质的影响[D]. 沈阳:沈阳农业大学,2023.
[3] SHANYING H E,HAIHUI G U O,ZHENLI H E,et al. Effects of a new-type cleaning agent and a plant growth regulator on phytoextraction of cadmium from a contaminated soil[J]. Pedosphere,2019,29(2):161-169.
[4] 王霞霞. 植物生长调节剂对马铃薯试管苗促生壮苗及干旱胁迫的调控[D]. 晋中:山西农业大学,2022.
[5] 郑先福,孙炳剑,郑昊. 己酸二乙氨基乙醇酯亚慢性经口毒性[J]. 农药,2005,44(11):6.
[6] 李跃伟,侯金丹,孙慕芳,等. 四种生长调节剂对玉米茎秆性状及抗倒伏性的影响[J]. 江苏农业学报,2023,39(2):377-382.
[7] 徐蒙可. DA-6对棉花种子萌发及幼苗光合特性的影响[D]. 石河子:石河子大学,2021.
[8] 韩冬,周伟鑫,张景云,等. 不同植物生长调节剂对大豆生长及产量的影响[J]. 黑龙江农业科学,2023(12):20-28.
[9] 袁晓婷,罗凯,刘姗姗,等. 化控对玉米带状间作大豆主茎分枝光合特性、物质积累及产量的影响[J]. 核农学报,2024,38(4):776-784.
[10] 张雪梅,王雨熙,朱沿舟,等. 胺鲜酯对树番茄幼苗养分吸收和生理指标的影响[J]. 云南农业大学学报(自然科学),2023,38(4):606-614.
[11] 余鹏,孟祥轩,余义和,等. 采前喷施胺鲜酯对采后‘巨峰’葡萄果实品质和活性氧代谢的影响[J]. 食品科学,2023,44(1):182-188.
[12] 陈胜雯. 胺鲜酯对桃生长、养分吸收及果实品质的影响[D]. 雅安:四川农业大学,2022.
[13] JIANG Y P,JIANG Y T,HE S,et al.Dissipation of diethyl aminoethyl hexanoate(DA-6)residues in pakchoi,cotton crops and soil[J]. Bulletin of Environmental Contamination and Toxicology,2012,88:533-537.
[14] 孔顺. 多介质中胺鲜酯的分析方法及应用研究[D]. 南京:东南大学,2020.
[15] LI M M,LIU X G,DONG F S,et al.Simultaneous determination of hexanoic acid 2-(diethylamino)ethyl ester and mepiquat chloride by ultra-performance liquid chromatography coupled to tandem mass spectrometry[J]. Analytical Methods,2012,4(11):3804-3809.
[16] 潘志明,周路明,黄琼,等. 食品中胺鲜酯的气相色谱—质谱联用仪测定方法研究[J]. 世界科技研究与发展,2015,37(2):134-137.
[17] 白雪梅,杨金川. QuEChERS—气相色谱—质谱法测定番茄中胺鲜酯的残留含量[J]. 安徽农业科学,2021,49(5):200-201,237.
[18] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. 实验室质量控制规范食品理化检测:GB/T27404—2008[S]. 北京:中国标准出版社,2008:3.
[19] RAJSKI Ł,LOZANO A,UCLÉS A,et al. Determination of pesticide residues in high oil vegetal commodities by using various multi-residue methods and clean-ups followed by liquid chromatography tandem mass spectrometry[J]. Journal of Chromatography A,2013,1304:109-120.
[20] QIN X,ZHAO L,HUANG Q,et al.Rapid multi-residue determination of pesticides in animal-derived food via modified QuEChERS sample preparation and GC/MS[J]. Food Analytical Methods,2018,11:1493-1500.
[21] 聂宏骞,易长文,王国玲,等. 生活饮用水中拟除虫菊酯类农药的空气辅助分散液液微萃取—气相色谱—质谱测定法[J]. 环境与健康杂志,2021,38(3):241-245.
[22] 陈佳楠,李丹,周一冉,等. 基于深共晶溶剂的液—液微萃取法结合液相色谱—串联质谱法检测枸杞中的10种农药残留[J]. 食品安全质量检测学报,2021,12(12):4793-4800.
[23] 史洪飞,徐伯芃,徐成鑫. QuEChERS—高效液相色谱—四极杆—飞行时间质谱法正负离子切换同时检测血液中的6种除草剂[J]. 分析科学学报,2024(2):219-224.
[24] 王秀丽,张梅超,倪瑞敏,等. QuEChERS—液相色谱—串联质谱法同时测定蔬菜中7种新烟碱类农药[J]. 农药,2024,63(4):254-260.
[25] 边海涛,董睿男,勇艳华,等. 高效液相色谱—串联质谱法同时测定谷物中10种HPPD抑制剂类除草剂及其代谢物[J]. 农药,2024,63(4):274-279.
[26] GUO J,LI M,LIU Y,et al.Residue and dietary risk assessment of chiral cyflumetofen in apple[J]. Molecules,2018,23(5):1060.

Determination of DA-6 residues in eggs and chicken tissues based on improved QuEChERS-UPLC-MS/MS

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