Abstract: Simultaneous quantitative analysis models of benzo[a]pyrene and benzo[b]fluoranthene were established in aqueous solution combining three dimensional fluorescence spectroscopy with N-way partial least squares. 18 mixed solution samples with different mass concentration ratios（The concentrations of benzo[a]pyrene and benzo[b]fluoranthene were 1~10×10^-7 g/L） were prepared, and three dimensional fluorescence spectra of all samples were collected by the LS-55 fluorescence spectrophotometer（PerkinElmer, USA）. Based on the three-dimensional fluorescence characteristics of single component and mixed solution, quantitative analysis models of benzo[a]pyrene and benzo[b]fluoranthene were established in water using N-way partial least squares. For the benzo[a]pyrene in water, the correlation coefficient between the predicted and actual concentration is 0.979, and the predicted standard deviation is 6.02×10^-7 g/L; for the benzo[b]fluoranthene in water, the correlation coefficient between the predicted and actual concentration is 0.952, and the predicted standard deviation is 7.76×10^-7 g/L. This study provides a theoretical and experimental basis for the detection of benzo[a]pyrene and benzo[b]fluoranthene in soil environments by laser-induced fluorescence.

Key words: three-dimensional fluorescence spectroscopy, benzo[a]pyrene, benzof[b]fluoranthene, water, N-way partial least squares