天津农学院学报 ›› 2025, Vol. 32 ›› Issue (3): 1-7.doi: 10.19640/j.cnki.jtau.2025.03.001

• •    下一篇

低温对小球藻固碳效能的影响及转录组学分析

张芮, 高金伟, 姜青青, 王钰晴, 张丹, 周文礼通信作者   

  1. 天津农学院 水产学院 天津市水产生态及养殖重点实验室,天津 300392
  • 收稿日期:2025-04-13 出版日期:2025-06-30 发布日期:2025-07-02
  • 通讯作者: 周文礼(1969—),男,研究员,博士,主要研究渔业资源与环境修复方向。E-mail:wenlizhou@tjau.edu.cn。
  • 作者简介:张芮(1997—),女,硕士在读,主要研究渔业资源与环境修复方向。E-mail:614089731@qq.com。
  • 基金资助:
    国家重点研发计划课题(2024YFD2401803); 天津市渔业绿色发展项目(YYLSFZ202501)

Physiological response and transcriptomic analysis of Chlorella vulgaris to cold stress

Zhang Rui, Gao Jinwei, Jiang Qingqing, Wang Yuqing, Zhang Dan, Zhou WenliCorresponding Author   

  1. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2025-04-13 Online:2025-06-30 Published:2025-07-02

摘要: 为探究低温保存时间对小球藻(Chlorella vulgaris)生理生化及相关机制的影响,本研究对低温保存10 d及90 d的小球藻进行复苏再培养,分析了藻细胞生长及固碳效能等指标,并借助高通量测序技术,筛选与光合作用及固碳效能相关的差异基因和代谢通路。结果显示:与对照组相比,10 d和90 d试验组的小球藻生长密度均在复苏第8天恢复至正常水平,固碳速率在第8天显著高于对照组(P<0.05),CA及Rubisco活性提升;KEGG通路注释表明,10 d试验组差异基因富集于DNA复制和卡尔文循环固碳等通路中,其中卡尔文循环固碳通路富集13个差异表达基因(Differentially Expressed Genes,DEGs);90 d试验组差异基因富集于氮代谢和精氨酸合成等代谢相关通路。

关键词: 小球藻, 低温, 生长, 光合作用, 代谢通路

Abstract: In order to investigate the effects of different cryopreservation times on the physiology and biochemistry of Chlorella vulgaris and related mechanisms, C. vulgaris was recovered and re-cultured after 10 d and 90 d of cryopreservation, and the indicators of algal cell growth and carbon sequestration efficacy were analysed, and differential genes and metabolic pathways related to photosynthesis and carbon sequestration efficacy were screened with the help of high-throughput sequencing technology. The results showed that compared with the control group. the growth density of Chlorella recovered to normal on the 8th day of recovery in both the 10 d and 90 d experimental groups, and the carbon sequestration rate was significantly higher than that of the control group on the 8th day(P<0.05), and the activities of CA and Rubisco were increased; the annotation of the KEGG pathway showed that the differential genes were enriched in the pathways of DNA replication and the Calvin cycle of carbon sequestration in the 10 d experimental group, among which the Calvin cycle carbon sequestration pathway was enriched with 13 DEGs; in the 90 d test group the differential genes were enriched in metabolism-related pathways such as nitrogen metabolism and arginine synthesis.

Key words: Chlorella vulgaris, low temperature, growth, photosynthetic, metabolic pathway

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