荧光光谱法快速检测土壤中菲浓度

doi:10.19640/j.cnki.jtau.2023.01.012

天津农学院学报 ›› 2023, Vol. 30 ›› Issue (1): 55-58.doi: 10.19640/j.cnki.jtau.2023.01.012

荧光光谱法快速检测土壤中菲浓度

张汉, 雷涛, 杨仁杰^通信作者, 董桂梅

天津农学院工程技术学院,天津 300392

收稿日期:2021-08-29 出版日期:2023-03-31 发布日期:2023-04-21
通讯作者: 杨仁杰（1978—）,男,教授,博士,主要从事光谱检测技术与应用方面的研究。E-mail：rjyang1978@163.com。
作者简介:张汉（1999—）,男,硕士在读,主要从事光谱检测方面的研究。E-mail：1377763695@qq.com。
基金资助:
国家自然科学基金项目（41771357,21607114,81471698）; 天津市研究生科研创新项目（2020YJSS126）

Rapid determination of phenanthrene in soil using fluorescence spectroscopy

Zhang Han, Lei Tao, Yang Renjie^{Corresponding Author}, Dong Guimei

College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300392, China

Received:2021-08-29 Online:2023-03-31 Published:2023-04-21

摘要/Abstract

摘要： 提出了一种利用荧光光谱法快速直接对土壤中多环芳烃进行分析的检测方法。以菲为研究对象,验证了荧光光谱法直接检测土壤中菲污染物的可行性。配制不同浓度菲土壤样品,并采用Perkin Elmer公司生产的LS-55型荧光分光光度计对菲土壤样品进行荧光光谱采集。通过对二维相关荧光谱的分析确定了409和430 nm处的荧光峰都来自土壤中的菲,485 nm的荧光峰来自土壤自身。在此基础上,分别基于409和430 nm处的荧光强度建立定量分析土壤中菲浓度的标准曲线,其对应的相关系数R²分别为0.98和0.99。选择7个样品建立标准曲线并对3个未知样品进行预测,其对应的平均预测误差分别为1.69%和6.71%。研究结果表明：基于荧光光谱法直接对土壤中的多环芳烃污染物检测是可行的。该研究对于实现土壤中PAHs污染物实时、现场荧光检测具有重要的意义。

关键词: 荧光光谱, 多环芳烃, 土壤, 菲, 快速检测

Abstract: A rapid and direct detection method of polycyclic aromatic hydrocarbons（PAHs）in soil using fluorescence spectrometry was proposed. Taking phenanthrene as the research object, the feasibility of direct detection of phenanthrene in soil was verified by fluorescence spectroscopy. Soil samples with different concentrations of phenanthrene were configured and fluorescence spectra of all samples were collected by using a LS-55 fluorescence spectrophotometer produced by Perkin Elmer. Through the analysis of two-dimensional correlation fluorescence spectra, it was determined that the fluorescence peaks at 409 and 430 nm were both from phenanthrene in the soil, and the fluorescence peak at 485 nm was from the soil itself. Standard curves for quantitative analysis of phenanthrene content in soil were established based on the fluorescence intensities at 409 and 430 nm, respectively. The corresponding correlation coefficients R² were 0.98 and 0.99, respectively. Seven samples were selected to establish standard curves with corresponding average errors of 1.69% and 6.71% for three unknown samples, respectively. The results showed that the direct detection of PAH contaminants in soil was feasible based on fluorescence spectrometry. This study is important for realizing real-time, in situ fluorescence detection of PAHs contaminants in soil.

Key words: fluorescence spectroscopy, polycyclic aromatic hydrocarbons, soil, phenanthrene, rapid detection

中图分类号:

O433

张汉, 雷涛, 杨仁杰, 董桂梅. 荧光光谱法快速检测土壤中菲浓度[J]. 天津农学院学报, 2023, 30(1): 55-58.

Zhang Han, Lei Tao, Yang Renjie, Dong Guimei. Rapid determination of phenanthrene in soil using fluorescence spectroscopy[J]. Journal of Tianjin Agricultural University, 2023, 30(1): 55-58.

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http://xuebao.tjau.edu.cn/CN/Y2023/V30/I1/55

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荧光光谱法快速检测土壤中菲浓度

Rapid determination of phenanthrene in soil using fluorescence spectroscopy

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