海参肠道微生物的形成与影响因素研究进展

doi:10.19640/j.cnki.jtau.2019.02.019

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (2): 84-88.doi: 10.19640/j.cnki.jtau.2019.02.019

海参肠道微生物的形成与影响因素研究进展

柴英辉, 范鑫昊, 刘胜男, 高金伟, 贾旭颖, 邵蓬, 周文礼^通信作者

天津农学院水产学院天津市水产生态及养殖重点实验室,天津 300384

收稿日期:2019-02-26 出版日期:2019-06-20 发布日期:2019-09-19
通讯作者: 周文礼（1969-）,男,研究员,博士,主要从事微藻资源化利用与生态学研究。E-mail：wenlizhou@tjau.edu.cn。
作者简介:柴英辉（1991-）,男,硕士在读,主要从事微藻资源化和水生态学研究。E-mail：949201278@qq.com。
基金资助:
天津市生物饵料校企协同创新重点实验室建设（17PTSYJC00170）; 天津市企业科技特派员项目（18JCTPJC67400）; 天津市现代农业产业技术体系创新团队建设资金（ITTFRS2017005）

Research progress on the formation and influencing factors of intestinal microorganisms of sea cucumber

CHAI Ying-hui, FAN Xin-hao, LIU Sheng-nan, GAO Jin-wei, JIA Xu-ying, SHAO Peng, ZHOU Wen-li

Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-02-26 Online:2019-06-20 Published:2019-09-19

摘要/Abstract

摘要： 随着海参养殖规模的扩大,许多养殖方面的问题逐渐凸显。海参养殖过程中的污染物如残饵粪便等分解产生有毒害作用的氨氮、亚硝酸盐等,可导致水质下降,严重降低海参的产量;由病原微生物引起的病害问题更是直接威胁着水产养殖的经济效益。虽然渔业资源与环境保护的力度一再增强,但海参养殖的病害还是频频发生,病害问题追溯到根源主要为微生物,因此,对海参肠道微生物的形成及其宿主调控进行综述,介绍肠道微生物早期的定植、微生物的功能和调控,为了解海参肠道微生物的形成机制及相关病害的预防和治疗等提供参考。

关键词: 海参, 病原微生物, 病害, 功能

Abstract: With the expansion of the scale of the cultivation of sea cucumber, many problems in the cultivation gradually become prominent. In the process of sea cucumber cultivation, pollutants such as residual bait excrement and other pollutants produce toxic ammonia nitrogen and nitrite, which can lead to the decline of water quality and seriously reduce the yield of sea cucumber. The disease caused by pathogenic microorganism directly threatens the economic benefit of aquaculture. Despite the strengthening of fishery resources and environmental protection, the disease of sea cucumber breeding still occurs frequently, and the disease is mainly traced back to microorganisms. Therefore, this paper summarized the formation and host regulation of intestinal microorganisms of sea cucumber, and introduced the early colonization, function and regulation of intestinal microorganisms, providing a reference for understanding the formation mechanism of intestinal microorganisms of sea cucumber as well as the prevention and treatment of related diseases.

Key words: sea cucumber, pathogenic microorganisms, disease, function

中图分类号:

S968.9

柴英辉, 范鑫昊, 刘胜男, 高金伟, 贾旭颖, 邵蓬, 周文礼. 海参肠道微生物的形成与影响因素研究进展[J]. 天津农学院学报, 2019, 26(2): 84-88.

CHAI Ying-hui, FAN Xin-hao, LIU Sheng-nan, GAO Jin-wei, JIA Xu-ying, SHAO Peng, ZHOU Wen-li. Research progress on the formation and influencing factors of intestinal microorganisms of sea cucumber[J]. Journal of Tianjin Agricultural University, 2019, 26(2): 84-88.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2019.02.019

http://xuebao.tjau.edu.cn/CN/Y2019/V26/I2/84

参考文献

[1] Bi Y,Yang R.Human gut microbiota,nutrition and health[J]. Chinese Science Bulletin,2018,64(3):260–271.
[2] 赵锁花,杨建德. 人感染布氏杆菌病的风险因素分析[J].天津农学院学报,2017,24(3):97–98,101.
[3] Llewellyn M S,Boutin S,Hoseinifar S H,et al.Teleost microbiomes: the state of the art in their characterization,manipulation and importance in aquaculture and fisheries[J]. Front Microbiol,2014,5:207.
[4] Hwang J S,Im C R,Im S H.Immune disorders and its correlation with gut microbiome[J]. Immune Netw,2012,12(4):129–138.
[5] Yu H, Gao Q,Dong S,et al.Regulation of dietary glutamine on the growth,intestinal function,immunity and antioxidant capacity of sea cucumber Apostichopus japonicus(Selenka)[J]. Fish Shellfish Immunol,2016,50:56–65.
[6] Garcia T,Otto K,Kjelleberg S,et al.Growth of Vibrio anguillarum in salmon intestinal mucus[J]. Appl Environ Microbiol,1997,63(3):1034–1039.
[7] Hansen G H,Olafsen J A.Bacterial colonization of cod (Gadus morhua L.)and halibut(Hippoglossus hippoglossus)eggs in marine aquaculture[J]. Appl Environ Microbiol,1989,55(6):1435–1446.
[8] Castro D,Pujalte M J,Lopez-Cortes L,et al.Vibrios isolated from the cultured manila clam(Ruditapes philippinarum):numerical taxonomy and antibacterial activities[J]. J Appl Microbiol,2002,93(3):438–447.
[9] Olafsen J A,Pujalte M J,Lopez-Cortes L,et al.Indigenous bacteria in hemolymph and tissues of marine bivalves at low temperatures[J]. Appl Environ Microbiol,1993,59(6):1848–1854.
[10] Wang X W,Xu J D,Zhao X F,et al.A shrimp C-type lectin inhibits proliferation of the hemolymph microbiota by maintaining the expression of antimicrobial peptides[J]. J Biol Chem,2014,289(17):11779–11790.
[11] Lokmer A,Xu J D,Zhao X F,et al.Spatial and temporal dynamics of Pacific oyster hemolymph microbiota across multiple scales[J]. Front Microbiol,2016,7:1367.
[12] Lokmer A,Mathias W K.Hemolymph microbiome of Pacific oysters in response to temperature,temperature stress and infection[J]. ISME J,2015,9(3):670–682.
[13] Yamazaki Y,Meirelles P M,Mino S,et al.Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps[J]. Sci Rep,2016,6:21631.
[14] Coteur G,Mellroth P,De Lefortery C,et al.Peptidoglycan recognition proteins with amidase activity in early deuterostomes (Echinodermata)[J]. Dev Comp Immunol,2007,31(8):790–804.
[15] Haug T,Kjuul A K,Styrvold O B,et al.Antibacterial activity in Strongylocentrotus droebachiensis(Echinoidea),Cucumaria frondosa(Holothuroidea),and Asterias rubens(Asteroidea)[J]. J Invertebr Pathol,2002,81(2):94-102.
[16] Osbourn A,Goss R J,Field R A.The saponins:polar isoprenoids with important and diverse biological activities[J]. Nat Prod Rep,2011,28(7):1261–1268.
[17] Collard M,Laitat K,Moulin L,et al.Buffer capacity of the coelomic fluid in echinoderms[J]. Comp Biochem Physiol A MolIntegr Physiol,2013,166(1):199–206.
[18] Dybas L,Fankboner P V.Holothurian survival strategies: mechanisms for the maintenance of a bacteriostatic environment in the coelomic cavity of the sea cucumber,Parastichopus californicus[J]. Dev Comp Immunol,1986,10(3):311–330.
[19] Jin J O,Shastina V V,Shin S W,et al.Differential effects of triterpene glycosides,frondoside A and cucumarioside A2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells[J]. FEBS Lett,2009,583(4):697–702.
[20] Nagase H,Enjyoji K,Shima M,et al.Effect of depolymerized holothurian glycosaminoglycan (DHG)on the activation of factor VIII and factor V by thrombin[J]. J Biochem,1996,119(1):63–69.
[21] Matranga V,Pinsino A,Celi M,et al.Monitoring chemical and physical stress using sea urchin immune cells[J]. Prog Mol Subcell Biol,2005,39:85-110.
[22] Enomoto M,Nakagawa S,Sawabe T.Microbial communities associated with holothurians: presence of unique bacteria in the coelomicfluid[J]. Microbes Environ,2012,27(3):300–305.
[23] 张凤萍,王印庚,李胜忠,等. 应用PCR方法检测刺参腐皮综合征病原——灿烂弧菌[J]. 海洋水产研究,2008(5):100–106.
[24] Lemos M L,Toranzo A E,Barja J L.Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds[J]. Microb Ecol,1985,11(2):149–163.
[25] Vasconcelos A A,Pomin V H.The sea as a rich source of structurally unique glycosaminoglycans and mimetics[J]. Microorganisms,2017,5(3):51.
[26] Grauso L,Yegdaneh A,Sharifi M,et al.Molecular networking-based analysis of cytotoxic saponins from sea cucumber holothuria atra[J]. Mar Drugs,2019,17(2):86.
[27] 丁斯予,王荦,徐瀚晨,等. 刺参肠道及养殖环境菌群结构与功能[J]. 生态学杂志,2019,38(1):210–220.
[28] Fu J,Yang D,Jin M,et al.Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation:role of different regions within the zebra fish intestinal tract,and impact on fish intestinal microbiota[J]. Mol Ecol,2017,26(19):5318–5333.
[29] Smith V J,Desbois A P,Dyrynda E A.Conventional and unconventional antimicrobials from fish,marine invertebrates and micro-algae[J]. Mar Drugs,2010,8(4):1213–1262.
[30] Sorum H,Roberts M C,Crosa J H.Identification and cloning of a tetracycline resistance gene from the fish pathogen Vibrio salmonicida[J]. Antimicrob Agents Chemother,1992,36(3):611–615.
[31] Xiong W,Sun Y,Zhang T,et al.Antibiotics,antibiotic resistance genes,and bacterial community composition in fresh water aquaculture environment in China[J]. Microb Ecol,2015,70(2):425–432.
[32] Shterzer N,Mizrahi I.The animal gut as a melting pot for horizontal gene transfer[J]. Can J Microbiol,2015,61(9):603–605.
[33] Kim D H,Brunt J,Austin B.Microbial diversity of intestinal contents and mucus in rainbow trout(Oncorhynchus mykiss)[J]. J Appl Microbiol,2007,102(6):1654-1664.
[34] Gaze W H,Abdouslam N,Hawkey P M,et al.Incidence of class 1 integrons in a quaternary ammonium compound- polluted environment[J]. Antimicrob Agents Chemother,2005,49(5):1802-1807.
[35] Plante C J,Coe K M,Plante R G.Isolation of surfactant- resistant bacteria from natural,surfactant-rich marine habitats[J]. Appl Environ Microbiol,2008,74(16):5093-5099.
[36] Yan F J,Tian X L,Dong S L,et al.Seasonal variation of functional diversity of aquatic microbial community in Apostichopus japonicus cultural pond[J]. Chinese Journal of Applied Ecology,2014,25(5):1499-1505.
[37] Gao F,Li F,Tan J,et al.Bacterial community composition in the gut content and ambient sediment of sea cucumber Apostichopus japonicus revealed by 16S rRNA gene pyrosequencing[J]. Plos One,2014,9(6):100092.
[38] Arena M P,Caggianiello G,Fiocco D,et al.Barley beta- glucans-containing food enhances probiotic performances of beneficial bacteria[J]. Int J Mol Sci,2014,15(2):3025-3039.
[39] 王光玉,冯亚利,张欣,等. 微生态制剂对刺参养殖的影响[J]. 饲料工业,2017,38(14):29–34.
[40] 赵慧慧,王忠菊,温泽民. 海参养殖中微生态制剂的应用探讨[J]. 南方农业,2018,12(5):98–99.
[41] 田功太,刘飞,段登选,等. EM菌对海参养殖水体理化因子的影响[J]. 水生态学杂志,2012,33(1):75-79.
[42] 彭宏宇. 微生物制剂在水产养殖中的应用研究[J]. 乡村科技,2018(30):97–98,100.
[43] Ding J,Dou Y,Wang Y,et al.Draft genome sequence of Vibrio fortis Dalian14 isolated from diseased sea urchin (Strongylocentrotus intermedius)[J]. Genome Announc,2014,2(4):e00409.
[44] Guardiola F A,Cuesta A,Meseguer J,et al.Risks of using antifouling biocides in aquaculture[J]. Int J MolSci,2012,13(2):1541–1560.
[45] Shah S Q,Ivanova L,Buschmann A H,et al.Antimicrobial resistance and antimicrobial resistance genes in marine bacteria from salmon aquaculture and non-aquaculture sites[J]. Environ Microbiol,2014,16(5):1310–1320.
[46] Domisch S,Kakouei K,Martínez-López J,et al.Social equity shapes zone-selection: Balancing aquatic biodiversity conservation and ecosystem services delivery in the transboundary Danube River Basin[J]. Sci Total Environ,2019,656:797–807.
[47] Wang H,Xue Z,Liu Z,et al.A novel C-type lectin from the sea cucumber Apostichopus japonicas(AjCTL-2)with preferential binding of d-galactose[J]. Fish Shellfish Immunol,2018,79:218–227.
[48] Su Q,Jin T,Yu Y,et al.Extracellular expression of a novel beta-agarase from Microbulbifer sp. Q7,isolated from the gut of sea cucumber[J]. AMB Express,2017,7(1):220.
[49] 刘燕霏,杨建德. 病原菌感染适应宿主相关机制研究进展[J]. 天津农学院学报,2018,25(1):90–93.

海参肠道微生物的形成与影响因素研究进展

Research progress on the formation and influencing factors of intestinal microorganisms of sea cucumber

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

本文评价

[1]	李建军, 王永, 孙芦苇, 丁巧玲. 9例猫肾功能衰竭的临床分析[J]. 天津农学院学报, 2024, 31(1): 83-87.
[2]	韩婧娥, 王欢欢, 李鹏程, 佘福泽, 黎肖昱, 秦顺义. 白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究[J]. 天津农学院学报, 2023, 30(3): 47-52.
[3]	苏丹, 邓永卓. 农作物病害图像识别研究进展和存在问题[J]. 天津农学院学报, 2023, 30(3): 75-79.
[4]	杨娇, 秦铭杉, 吕海军, 颜雪涛, 杨贵军, 杨琳燕. 基于γ-Fe₂O₃磁性纳米微球的过氧化物模拟酶研究[J]. 天津农学院学报, 2022, 29(1): 47-51.
[5]	陈景, 陈建华, 刘凯, 吴海清. 磷脂酶D特性及其在功能性磷脂生产中的应用[J]. 天津农学院学报, 2021, 28(2): 79-84.
[6]	丁巧玲, 曹雅洁, 陈姝祁, 毛晓梦, 李硕, 李建军. 一例猫肝功能衰竭的诊断与治疗[J]. 天津农学院学报, 2019, 26(3): 71-73.
[7]	路通, 杨秀荣, 霍建飞, 孙淑琴, 刘春艳, 郝永娟. 天津地区水稻苗期病害的防治研究[J]. 天津农学院学报, 2019, 26(2): 46-50.
[8]	薄香兰, 刘兴, 窦勇, 贾旭颖, 高金伟, 周文礼. 小球藻生长因子CGF的研究与应用进展[J]. 天津农学院学报, 2018, 25(1): 86-89.
[9]	韩卓然, 孙敬锋, 刘军锋, 郭永军, 邢克智, 吕爱军, YEONGYiksung, 尤宏争. 毛蚶血细胞分类及吞噬功能研究[J]. 天津农学院学报, 2017, 24(3): 50-54.
[10]	纪思琪, 吴芳, 李乃祥. 基于决策树的蔬菜病害静态预警模型[J]. 天津农学院学报, 2017, 24(2): 77-80.
[11]	李坤怿, 袁文娅, 王鹏文. DNA甲基化的植物学意义及其研究方法[J]. 天津农学院学报, 2017, 24(1): 77-82.
[12]	陈招荣, 梁红春, 阎国荣. 服务沿海都市型农业的果树学研究生培养实践与探讨——以天津农学院为例[J]. 天津农学院学报, 2016, 23(1): 71-74.
[13]	胡烨, 马吉飞, 赵瑞利. 抗菌肽作为饲料添加剂的研究进展[J]. 天津农学院学报, 2013, 20(4): 48-51.
[14]	庞玥, 张树林, 毕相东. 蓝藻光合膜蛋白复合体结构与功能研究进展[J]. 天津农学院学报, 2013, 20(3): 45-51.