蓝藻光合膜蛋白复合体结构与功能研究进展

天津农学院学报 ›› 2013, Vol. 20 ›› Issue (3): 45-51.

蓝藻光合膜蛋白复合体结构与功能研究进展

庞玥, 张树林^通信作者, 毕相东

天津农学院天津市水产生态及养殖重点实验室,天津 300384

收稿日期:2013-03-06 发布日期:2019-10-21
通讯作者: 张树林（1963-）,男,黑龙江齐齐哈尔人,教授,博士,研究方向为水生生物学。E-mail：shulin63@sina. com。
作者简介:庞玥（1988-）,女,天津市人,硕士在读,研究方向为水生生物和水环境调控技术。E-mail：894900363@qq. com。
基金资助:
国家自然科学基金面上项目"中草药小檗碱对铜绿微囊藻光合作用影响的研究"（31170442）; 天津市应用基础与前沿技术研究计划重点项目"中草药黄连抑制铜绿微囊藻生长的机理研究"（10JCZDJC18000）

Progress of Structure and Function of Photosynthetic Membrane Protein Complexes in Cyanobacteria

PANG Yue, ZHANG Shu-lin^{Corresponding Author}, BI Xiang-dong

Key Laboratary of Aqua-ecology and Aquaculture of Tianjin, Tianjin Agricultural University, Tianjin 300384, China

Received:2013-03-06 Published:2019-10-21

摘要/Abstract

摘要： 光合作用是蓝藻最基本、最重要的生态生理特征。各种膜蛋白复合体在光合作用中执行着重要的生物化学功能。本文对蓝藻5种膜蛋白复合体（PSI复合体、PSII复合体、细胞色素b₆f复合体、ATP合酶复合体和NAD（P）H脱氢酶复合体）的结构与功能进行了系统归纳,并对下一步的研究工作进行了展望。

关键词: 蓝藻, 光合作用, 膜蛋白复合体, 结构, 功能

Abstract: Photosynthesis is the most basic and important physiological and ecological characteristics of cyanobacteria. Membrane protein complexes perform an important biochemical function in photosynthesis. It is introduced the structure and function of five photosynthetic membrane protein complexes (photosystemⅠ, photosystemⅡ, cytochrome b₆f, ATP synthase and NAD(P)H dehydrogenase) in cyanobacteria, and an outlook for the later study is made.

Key words: cyanobacteria, photosynthesis, membrane protein complexes, structure, function

中图分类号:

Q945

庞玥, 张树林, 毕相东. 蓝藻光合膜蛋白复合体结构与功能研究进展[J]. 天津农学院学报, 2013, 20(3): 45-51.

PANG Yue, ZHANG Shu-lin, BI Xiang-dong. Progress of Structure and Function of Photosynthetic Membrane Protein Complexes in Cyanobacteria[J]. Journal of Tianjin Agricultural University, 2013, 20(3): 45-51.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2013/V20/I3/45

参考文献

[1] 李艳红. 环境因子对铜绿微囊藻生长和光合作用的影响[D]. 南昌:南昌大学,2010.
[2] 汪星. 蓝藻光合作用光系统Ⅱ的蛋白质工程与调控研究[D]. 武汉:华中科技大学,2011.
[3] 陈莲花. 表面活性剂对铜绿微囊藻生长及光合作用的影响[D]. 南昌:南昌大学,2008.
[4] 段伟. 低温条件下叶绿体NAD(P)H脱氢酶复合体的生理功能及其对光质的响应[D]. 山东:山东农业大学,2004.
[5] Berger S,Ellersiek U,Steinmuller K.Cyanobacteria contain a mitochondrial complex I homologous NADH- dehydrogenase[J]. FEBS Lett,1991,286(1-2):129-132.
[6] Berger S,Ellersiek U,Kinzelt D,et al.Immuno- purification of asubcomplex of the NAD(P)H- plastoquinone-oxidoreductase from the cyanobacterium Synechocystis sp. PCC 6803[J]. FEBS Lett,1993,326(1-3):246-250.
[7] 杜林方. 光合作用研究的一些进展[J]. 21世纪青年学者论坛,2003,21(1):58-62.
[8] Hladik J,Sofrova D.Does the trimeric form of the Photosystem I reaction center of cyanobacteria in vivo exist?[J]. Photosynth Res,1991,29:171-175.
[9] Krauss N,Hinrichs W,Witt I,et al.Three-dimensional structure of system I of photosynthesis at 6 A resolution[J]. Nature,1993,361(28):326-361.
[10] 胡朝辉. 高温和光照对光系统Ⅰ结构和功能的影响[D]. 北京:中国科学院研究生院,2004.
[11] 徐晓燕. N-苯基-2-萘胺对小球藻的毒性机制研究[D]. 杭州:浙江工业大学,2009.
[12] 陈金灶. 蓝藻光合系统电子传递蛋白(Fd)突变体的初步研究[D]. 厦门:厦门大学,2006.
[13] 柳斌. 蓝藻的光合作用与氢能开发[J]. 中学生物学,2006,22(5):9.
[14] Nield J,Kruse O,Ruprecht J,et al.3D structure of Chlamydomonas reinhardtii and Synechococcus elongatus photosystem II complexes allow for comparison of their OEC organization[J]. J Biol Chem,2000,275(36):27940-27946.
[15] Kern J,Renger G.Photosystem Ⅱ:structure and mechanism of the water: plastoquinone oxidoreductase[J]. Photosyn Res,2007,94(2-3):183-202.
[16] Loll B,Kern J,Saenger W,et al.Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem Ⅱ[J]. Nature,2005,438(15):1040-1044.
[17] Ferreira K N,Iverson T M,Maghlaoui K,et al.Architecture of the photosynthetic oxygenevolving center[J]. Science,2004,303(5665):1831-1838.
[18] Guskov A,Kern J,Gabdulkhakov A,et al.Cyanobacterial photosystem Ⅱ at 2.9-Å resolution and the role of quinines, lipids, channels and chloride[J]. Nature Struct Biol and Mol Biol,2009,16:334-342.
[19] Stewart D H,Brudvig G W.Cytochrome b559 of photosystem Ⅱ[J]. Biochim Biophys Acta,1998,1367(1-3):63-87.
[20] 吴乃虎,方晓华,施晓梅,等. 高粱叶绿体psbA基因的结构特征及其5'-非编码区的调控效应[J]. 中国科学(C辑),1999,29(4):397-406.
[21] Sippolak,Aro E M. Expression of psbA genes is regulated at multiple levels in the cyanobacterium Synechococcus sp. PCC 7942[J]. Photochemistry and Photobiology,2000,71(6):706-714.
[22] Constant S,Perewoska I,Alfonso M,et al.Expression of the psbA gene during photoinhibition and recovery in Synechocystis PCC 6714: inhibition and damage of transcriptional and translational machinery prevent the restoration of photosystem Ⅱ activity[J]. Plant Molecular Biology,1997,34(1):1-13.
[23] Mohamed A,Eriksson J,Osiewacz H D,et al.Differential expression of the psbA genes in the cyanobacterium Synechocystis 6803[J]. Molecular and General Genetics,1993,238(1-2):161-168.
[24] Summerfield T C,Toepel J,Sherman L A.Low-oxygen induction of normally cryptic psbA genes in cyanobacteria[J]. Biochemistry,2008,47(49):12939-12941.
[25] KÓS P B,Deak Z,Cheregi O,et al. Differential regulation of psbA and psbD gene expression, and the role of the different D1 protein copies in the cyanobacterium Thermosynechococcus elongatus BP-1[J]. Biochimicaet Biophysica Acta,2008,1777(1):74-83.
[26] 李想. 集胞藻PCC6803未知基因slr1122功能分析[D]. 武汉:华中农业大学,2011.
[27] De Las Rivas J,Balsera M,Barber J. Evolution of oxygenic photosynthesis: genome-wide analysis of the OEC extrinsic proteins[J]. Trends Plant Sci,2004,9(1):18-25.
[28] De Las Rivas J,Barber J. Analyses of the structure of the PsbO protein and its implications[J]. Photosyn Res,2004,81(3):329-343.
[29] Kashino Y,Lauber W M,Carroll J A,et al.Proteomic analysis of a highly active Photosystem II preparation from the cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel polypeptides[J]. Biochemistry,2002,41:8004-8012.
[30] Liu L N,Chen X L,Zhang Y Z,et al.Characterization, structure and function of linker polypeptides in phycobilisomes of cyanobacteria and red alage: an overview[J]. Biochim Biophys Acta,2005,1708(2):133-142.
[31] 苏海楠. 蓝藻与红藻中藻胆蛋白的活性构象研究[D]. 济南:山东大学,2010.
[32] Glazer A N.Phycobilisomes:a macromolecular complex optimized for light energy transfer[J]. Biochim Acta,1984,768:29-51.
[33] MacColl R. All ophycocyanin and energy transfer[J]. Biochim Biophys Acta,2004,1657(2-3):73-81.
[34] 容寿榆,俞国强,单晓亮. 蓝藻藻胆体与菠菜光系统Ⅱ颗粒之间的能量传递[J]. 植物学报,1998,40(7):622-626.
[35] 李宾兴. 假根羽藻细胞色素b₆f 蛋白复合体中α-胡萝卜素的结构与功能[D]. 北京:中国科学院研究生院,2005.
[36] Hope A B.The choroplast cytochrome b₆f complex: A critical focus on function[J]. Biochim Biophys Acta,1993,1143:1-22.
[37] Hamel P,Olive J,Pierre Y,et al.A new subunit of cytochrome b₆f complex undergoes reversible phosphory- lation upon state transition[J]. J Biol Chem,2000,275(22):17072-17079.
[38] Boekema E J,Boonstra A F,Dekker J P,et al.Electron microscopic structural analysis of Photosystem I,Photosystem II,and the cytochrome b₆f complex from green plants and cyanobacteria[J]. Biomembr,1994,26(1):17-29.
[39] Zhang H,Huang D,Cramer W A.Stoichiometrically bound β-carotene in the cytochrome b₆f complex of oxygenic photosynthesis protects against oxygen damage[J]. J Biol Chem,1999,274(3):1581-1587.
[40] Wollman F A,Minai L,Nechushtai R.The biogenesis and assembly of photosynthetic proteins in thylakoid membranes[J]. Biochin Biophys Acta,1999,1411(1999):21-85.
[41] Leuchen U,Gogol E P,Capaldi R A.Structure of the ATP synthase complex of Escherichia coli from cryoelectron microscopy[J]. Biochemistry,1990,29(22):5339-5343.
[42] Lemarire C,Wollmam F A.The chloroplast ATP synthase in Chlamydomonasreinhardtii[J]. J Biol Chem,1989,264(17):10235-10242.
[43] Schemidt R A,Hsu D K,Deckers-Hebestreit G,et al.The effects of an atpE ribosomebinding site mutation on the stoichiometry of the c subunit in the F₁F₀ ATPase of Escherichia coli[J]. Arch Biochem Biophys,1995,323(2):423-428.
[44] Chen G,Jagendorf A T.Chloroplast molecular chaperone assisted refolding and reconstitution of active multisubunit couplling factor CF1 core[J]. Proc Natl Acad Sci USA,1994,91(24):11497-11501.
[45] Ohkawa H,Pakrasi H B,Ogawa T.Two types of functionally distinct NAD(P)H dehydrogenases in Synechocystis sp. strain PCC 6803[J]. J Biol Chem,2000,275(41):31630-31634.
[46] Alpes I,Scherer S,BÖger P.The respiratory NADH dehydrogenase of the cyanobacterium Anabaena variabilis: purification and characterization[J]. Biochim Biophys Acta,1989,973(1):41-46.
[47] Howitt C A,Mith G D,Day D A.Cyanide-insensitive oxygen uptake and pyridine nucleotide dehydrogenase in the cyanobacterium Anabaena PCC 7120[J]. Biochim Biophys Acta,1993,1141(2-3):313-320.
[48] Howitt C A,Udall P K,,Vermaas W F.Type 2 NADH dehydrogenases in the cyanobacterium Synechocystis sp. strain PCC 6803 are involved in regulation rather than respiration[J]. J Bacteriol,1999,181(13):3994-4003.
[49] Viljoen C C,Cloete F,Scott W E.Isolation and characterization of an NAD(P)H dehydrogenase from the cyanobacterium,Microcystis aeruginosa[J]. Biochim Biophys Acta,1985,827(3):247-259.
[50] Mi H,Endo T,Schreiber U,et al.Electron donation from cyclic and respiratory flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobaterium Synechocystis PCC 6803[J]. Plant Cell Physiol,1992,33(8):1233-1237.
[51] Mi H,Endo T,Ogawa T,et al.Thylakoid membrane-bound, NADPH-specific pyridine nucleo tide dehydrogenase comples mediates cyclic electron transport in the cyanobacterium Synechocystis PCC 6803[J]. Plant Cell Physiol,1995,36(4):661-668.
[52] Klughammer B,Sultemeyer D,Badger M R,et al.The involvement of NAD(P)H dehydrogenase subunits,NdhD3 and NdhF3,in high affinity CO₂ uptake in Synechococcus sp. PCC 7002 gives evidence for multiple NDH complexes with specific roles in cyanobacteria[J]. Mol Microbiol,1999,32(6):1305-1315.
[53] Ohkawa H,Price G D,Badger M R,et al.Mutation of ndh genes leads to inhibition of CO₂ uptake rather than HCO₃- uptake in Synechocystis sp. strain PCC 6803[J]. J Bacteriol,2000,182(9):2591-2596.
[54] Tanaka Y,Katada S,Ishikawa H,et al.Electron flow from NAD(P)H dehydrogenase to photosystem I is required for adaptation to salt shock in the cyanobacterium Synechocystis sp. PCC 6803[J]. Plant Cell Physiol,1997,38(12):1311-1318.
[55] Deng Y,Ye J,Mi H.Effects of low CO₂ on NAD(P)H dehydrogenase,a mediator of cyclic electron transport around photosystem I in the cyanobacterium Synechocystis PCC 6803[J]. Plant Cell Physiol,2003,44(5):534-540.
[56] Kaplan A,Reinhold L.The CO₂ concentrating mechanisms in photosynthetic microorganisms[J]. Annu Rev Plant Physiol Plant Mol Biol,1999,50:539-570.
[57] Tchernov D,Helman Y,Keren N,et al.Passive entry of CO₂ and its energy-dependent intracellular conversion to HCO₃- in cyanobacteria are driven by a photosystem I-generated ΔμHP+P[J]. J Biol Chem,2001,276(26):23450-23455.
[58] Hibino T,Lee B H,Rai A K,et al.Salt enhances photosystem I content and cyclic electron flow via NAD(P)H dehydrogenase in the halotolerant cyanobacterium Aphanothece halophytica[J]. Aust J Plant Physiol,1996,23(3):321-330.
[59] Canaani O.The role of cyclic electron flow around photosystem I and exacitation energy distribution between the photosystems upon acclimation to high ionic strength in Dunaliela salina[J]. Photochem Photobiol,1990,52(3):591-599.
[60] Moresco J J,Carvalho P C,Yates Ⅲ J R. Identifying components of protein complexes in C. elegans using co-immunoprecipitation and mass spectrometry[J]. Proteomics,2010,73(11):2198-2204.
[61] Xu X,Song Y,Li Y,et al.The tandem affinity purification method: an efficient system for protein complex purification and protein interaction identification[J]. Protein Expr Purif,2010,72(2):149-156.
[62] Daigo K,Kawamura T,Ohta Y,et al.Proteomic analysis of native hepatocyte nuclear factor 4 alpha( HNF4 alpha)isoforms, phosphorylation status, and interactive cofactors[J]. J Biol Chem,2010,286(1):674-686.
[63] Dresler J,Klimentova J,Stulik J.Bacterial protein complexes investigation using blue native PAGE[J]. Microbiol Res,2011,166(1):47-62.
[64] 谢浩,郭小明. 融合标签技术在膜蛋白结构研究中的应用[J]. 生物技术通讯,2009,20(1):138-142.
[65] 姜爽,王晓波,袭荣刚,等. 原子力显微技术在膜蛋白研究中的应用[J]. 军事医学科学院院刊,2010,34(5):485-488.