天津农学院学报 ›› 2021, Vol. 28 ›› Issue (4): 6-10.doi: 10.19640/j.cnki.jtau.2021.04.002

• 研究与简报 • 上一篇    下一篇

新疆野苹果多倍体诱导及叶片特征分析

张丞慧, 李凯铭, 赵琪, 于洁, 贡嘎益西, 胡宝全通信作者   

  1. 天津农学院 园艺园林学院,天津 300392
  • 收稿日期:2020-07-08 出版日期:2021-12-31 发布日期:2022-01-05
  • 通讯作者: 胡宝全(1982—),男,助理研究员,博士,主要从事林木遗传育种方面的研究。E-mail:baoquan-hu@126.com。
  • 作者简介:张丞慧(1998—),男,本科在读,主要从事林木倍性育种方面的研究。E-mail:18149296066@126.com。
  • 基金资助:
    天津市大学生创新创业训练计划项目(201810061251)

Induction of polyploid Malus sieversii(Ledeb.)Roem. and characteristic analysis of its leaves

Zhang Chenghui, Li Kaiming, Zhao Qi, Yu Jie, Gongga Yixi, Hu BaoquanCorresponding Author   

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2020-07-08 Online:2021-12-31 Published:2022-01-05

摘要: 新疆野苹果是苹果重要的野生资源和砧木,挖掘其利用方法有利于苹果育种工作的进一步开展。本研究采用新疆野苹果实生幼苗为材料,通过不同浓度秋水仙素的浸渍处理诱导四倍体,并观察其叶片特征结构的变化。结果表明,0.2%的秋水仙素处理幼苗1 d能取得较好的诱导效果,新疆野苹果四倍体相对于二倍体,其叶片加厚,气孔密度降低,叶绿素含量增加,叶片解剖结构发生变化并导致叶片紧实度增加、疏松度下降,表现出高抗逆性的叶片特征。研究结果可为新疆野苹果种质资源的开发提供新途径,并为苹果新型优质砧木的培育和苹果的倍性育种工作提供新的参考和理论支持。

关键词: 新疆野苹果, 四倍体, 秋水仙素, 叶片, 解剖结构

Abstract: Malus sieversii is a rich natural resource and rootstock of apple tree. Mining its utilization method is beneficial to the further development of apple breeding. In this study, tetraploid Malus sieversii was induced by soaking stem apex of seedlings with different concentrations of colchicine, and changes in the characteristic structure of the leaves in tetraploid Malus sieversii was analyzed. The results showed that 0.2% colchicine treatment for 1 day achieved better induction effect. The leaves thickness and chlorophyll content of tetraploid increased, but stomatal density decreased, compared with diploid. Changes in anatomical structure of tetraploid leaves significantly resulted the increase of the cell tense ratio and the decrease of spongy ratio, which were was the leaf characteristics with high resistance. The results can provide a new way for the development of Malus sieversii germplasm resource, new reference and theoretical support for the cultivation of new high-quality apple rootstock and polyploid breeding work of apple.

Key words: Malus sieversii(Ledeb.)Roem., tetraploid, colchicine, leaves, anatomical structure

中图分类号: