天津农学院学报 ›› 2012, Vol. 19 ›› Issue (2): 30-33.

• 研究与简报 • 上一篇    下一篇

菩提树组织培养技术研究

王雅稚1, 孙宁1,通讯作者, 张磊1, 陈小强1, 许燕萍2   

  1. 1. 天津农学院 农学系,天津 300384;
    2. 天津滨海中新生态农业发展有限公司,天津 300480
  • 收稿日期:2012-05-08 出版日期:2012-06-20 发布日期:2019-11-12
  • 通讯作者: 孙宁(1979-),女,河北承德人,高级实验师,硕士,主要从事植物细胞工程方面的研究工作。E-mail:sning79@sina.com。
  • 作者简介:王雅稚(1990-),女,天津市人,本科在读,主要从事种子科学与工程方面的研究。联系电话:(022)23781298。
  • 基金资助:
    天津市科技支撑计划项目“非洲紫罗兰新品种的引进及培育技术的研究”(09ZCKFNC01600); 天津市滨海新区科技计划项目“名贵花卉、苗木新品种引进、快繁及设施栽培技术的研究”(2010-BK130049); 天津农学院大学生科技创新活动项目“菩提树组织培养无性快速繁殖技术的研究”(无编号)

Tissue Culture of Ficus religiosa

WANG Ya-zhi1, SUN Ning1, Corresponding Author, ZHANG Lei, CHEN Xiao-qiang1, XU Yan-ping2   

  1. 1. Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China;
    2. Tanjin Binhai New Ecological Agriculture Development CO., LTD, Tianjin 300480, China
  • Received:2012-05-08 Online:2012-06-20 Published:2019-11-12

摘要: 以菩提树的带芽茎段为外植体,研究了不同外植体消毒时间及外源激素水平对菩提树组织培养的影响。试验结果表明,外植体以0.1%的HgCl2消毒10 min可获得较低的污染率和较高的成活率;初代培养与分化增殖均采用MS为基本培养基,适宜的激素组合分别为NAA 0.1 mg/L+6-BA 1.0 mg/L、NAA 0.3 mg/L+6-BA 1.0~2.0 mg/L;试管苗生根培养以1/2 MS+NAA 0.5 mg/L为最佳,生根率达92.5%,植株生长健壮,适于移栽。污染的试管苗在移栽前进行灭菌药剂浸根处理,也可达到较高的成活率,可避免种苗的浪费。

关键词: 菩提树, 茎段, 组织培养

Abstract: Selecting the stem segment with buds as explants, the tissue culture technologies of Ficus religiosa were studied in the experiment. The results show that the optimum doused time was 8 to 10 min in 0.1% HgCl2, MS+NAA 0.1 mg/L+6-BA 1.0 mg/L was the best medium for inducing axillary buds, the proliferation coefficient was 4.5 on the medium of MS+NAA 0.3 mg/L+6-BA 1.0~2.0 mg/L, the suitable medium for rooting was 1/2 MS+NAA 0.5 mg/L, and the rate of rooting was 92.5%. The higher survival rate could be obtained, with the roots of contaminative plantlets being immersed in sterilized pharmaceutics before transplantation.

Key words: Ficus religiosa, stem segment, tissue culture

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