硬葡聚糖的提取条件及抗氧化性研究

doi:10.19640/j.cnki.jtau.2026.05.002

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (5): 9-16.doi: 10.19640/j.cnki.jtau.2026.05.002

硬葡聚糖的提取条件及抗氧化性研究

程岩, 何俣卓, 王冬宁, 田孝波, 黄亮^通信作者

天津农学院农学与资源环境学院,天津 300392

收稿日期:2025-03-10 发布日期:2026-11-02
通讯作者: 黄亮（1983—）,男,教授,博士,主要从事菌类作物方面的研究。E-mail：huangliang@tjau.edu.cn。
作者简介:程岩（2002—）,女,硕士在读,主要从事农艺与种业方面的研究。E-mail：18322507496@163.com。
基金资助:
天津市蔬菜产业技术体系创新团队（ITTVRS2026011）

Extraction conditions and antioxidant properties of scleroglucan

Cheng Yan, He Yuzhuo, Wang Dongning, Tian Xiaobo, Huang Liang^{Corresponding Author}

College of Agronomy and Resource Environmental, Tianjin Agricultural University, Tianjin 300392, China

Received:2025-03-10 Published:2026-11-02

摘要/Abstract

摘要： 为优化硬葡聚糖的提取与脱色工艺并开展产品抗氧化性分析,提升硬葡聚糖的产量和质量,采用醇沉法提取硬葡聚糖,通过苯酚-硫酸法测得原发酵液中硬葡聚糖含量为0.165 mg/mL,并确定最佳发酵液-无水乙醇体积比为1∶2,此时多糖保留率最高,为96.66%。用小颗粒活性炭、大孔树脂D101和大孔树脂S8进行脱色条件的筛选,从脱色温度、脱色时间、pH、脱色材料种类和脱色材料添加量等方面进行单因素试验。结果表明：小颗粒活性炭脱色的最佳条件为温度40 ℃,时间50 min,pH=4.0,添加量2.0%;大孔树脂D101为温度30 ℃,时间30 min,pH=3,添加量为13.33%;大孔树脂S8为温度30 ℃,时间60 min,pH=4,添加量为30%。在抗氧化活性方面,当硬葡聚糖质量浓度为6 mg/mL时,其在700 nm处的吸光度值为0.329;当质量浓度为3 mg/mL时,对DPPH自由基和超氧阴离子自由基（O₂^·-）的清除率分别为96.17%和35.76%;对羟基自由基（·OH）的清除能力受浓度影响较小,清除率约99.44%,说明硬葡聚糖具有一定的体外抗氧化能力。此外,75%燕麦提取物（对照）各抗氧化指标值均低于硬葡聚糖,进一步说明硬葡聚糖具有更好的抗氧化活性。本研究为进一步开发和利用硬葡聚糖提供了理论依据,具有一定的经济价值和广阔的应用前景。

关键词: 硬葡聚糖, 醇沉提取, 多糖脱色, 抗氧化活性

Abstract: In order to optimize the extraction and decolorization processes of scleroglucan and evaluate the antioxidant properties of the product so as to improve the yield and quality of scleroglucan, the alcohol-precipitation method was adopted for scleroglucan extraction. The main results were as follows: the content of scleroglucan was 0.165 mg/mL by phenol-sulfuric acid method, and the polysaccharide retention rate was 96.66% when the volume ratio of fermentation broth to anhydrous ethanol was 1∶2 by alcohol precipitation method. Small granular activated carbon, macroporous resin D101 and macroporous resin S8 were used to screen the decolorization conditions. Single factor tests were carried out from five aspects: decolorization temperature, decolorization time, pH and the type of decolorizing material and additive amount of decoloring material. The optimal conditions for small granular activated carbon decolorization were 40 ℃, 50 min and the amount of pH 4.0 and additive amount of 2.0%. The optimal conditions for macroporous resin D101 are 30 ℃, 30 min, pH 3 and 13.33%. The best condition for macroporous resin S8 is 30 ℃, 60 min, pH 4 and 30% addition. In the study of the antioxidant activity of scleroglucan, when the concentration of scleroglucan was 6 mg/mL, the absorbance value was about 0.329 at 700 nm; when the concentration of scleroglucan was 3 mg/mL, the scavenging rates of DPPH and superoxide anion radical were 96.17% and 35.76%, respectively. The scavenging ability of scleroglucan to hydroxyl free radical was little related to the concentration, and the clearance rate can reach about 99.44%. The results showed that scleroglucan had certain antioxidant capacity in vitro. In addition, the values of 75% oat extract as the control were lower than that of scleroglucan, which indicated that scleroglucan had better antioxidant properties, which provides a theoretical basis for further development and utilization of scleroglucan, and has excellent economic value and application prospects.

Key words: scleroglucan, alcohol precipitation extraction, polysaccharide decolorization, antioxidant activity

中图分类号:

TQ929.2

程岩, 何俣卓, 王冬宁, 田孝波, 黄亮. 硬葡聚糖的提取条件及抗氧化性研究[J]. 天津农学院学报, 2026, 33(5): 9-16.

Cheng Yan, He Yuzhuo, Wang Dongning, Tian Xiaobo, Huang Liang. Extraction conditions and antioxidant properties of scleroglucan[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 9-16.

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http://xuebao.tjau.edu.cn/CN/Y2026/V33/I5/9

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硬葡聚糖的提取条件及抗氧化性研究

Extraction conditions and antioxidant properties of scleroglucan

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