天津农学院学报 ›› 2021, Vol. 28 ›› Issue (4): 27-30.doi: 10.19640/j.cnki.jtau.2021.04.007

• 研究与简报 • 上一篇    下一篇

家兔气管支气管管道铸型标本制作三种腐蚀方法的比较

吕嘉琪, 诸葛增玉通信作者, 姚雨欣, 沈枸松, 甄珍   

  1. 天津农学院 动物科学与动物医学学院,天津 300392
  • 收稿日期:2020-01-15 出版日期:2021-12-31 发布日期:2022-01-05
  • 通讯作者: 诸葛增玉(1983—),女,讲师,博士,主要从事动物解剖学教学研究工作。E-mail:zhugezy@163.com。
  • 作者简介:吕嘉琪(1997—),女,本科在读,主要从事动物医学专业。E-mail:775747398@qq.com。

Comparison of three corrosion methods for cast specimens of tracheobronchial duct in rabbits

Lü Jiaqi, Zhuge ZengyuCorresponding Author, Yao Yuxin, Shen Jusong, Zhen Zhen   

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2020-01-15 Online:2021-12-31 Published:2022-01-05

摘要: 找出有效且简便易行的家兔肺脏气管支气管树铸型标本模型制作方法,为动物医学相关的教学、科研及临床实践提供真实严谨的解剖学形态依据。使用自凝牙托粉与牙托水配制成填充剂,从喉口处灌注进入家兔肺部,凝固后分别使用氢氧化钠、硫酸和盐酸腐蚀组织得到肺部气管支气管树铸型,比较不同腐蚀方法的效果。三种腐蚀方法均得到良好的铸型标本,使用氢氧化钠溶液腐蚀标本只需放入相同溶液再次浸泡;硫酸溶液腐蚀的标本偏黑色,需要再次漂白;盐酸溶液腐蚀的标本有残留脂类需用碱性液体浸泡清除。使用自凝牙托粉与牙托水按1.0∶1.5的比例填充灌注肺脏后,用50%氢氧化钠腐蚀组织,能够最便捷地得到形态完整、弹性适中、洁净光滑的呼吸道铸型标本,氢氧化钠溶液腐蚀标本无需配备其他溶液、物质不易挥发,是三种腐蚀方法中最便捷、安全的方法。

关键词: 家兔, 气管支气管, 标本, 铸型, 腐蚀方法

Abstract: To find out an effective and simple method to make the casting specimen of rabbit’s pulmonary trachea and bronchial tree, so as to provide a real and rigorous anatomical basis for veterinary related teaching, scientific research and clinical practice, three corrosion methods were used in this research. Self-solidifying denture powder and denture water were used to prepare fillers in proportion, and then perfusion from the throat into the lungs of rabbits. After the filler solidified, Sodium hydroxide solution, sulfuric acid solution and hydrochloric acid solution were used to corrode the lung tissues. The results showed that all of the three corrosion methods could get good cast specimen. The specimen corroded by sodium hydroxide solution only needed to be immersed in the same solution again; The specimens corroded by sulfuric acid solution were black and needed to be bleached again; The specimens corroded by hydrochloric acid solution needed to be immersed in alkaline solution to remove the residual lipids. After filling the lung with self-coagulating dental powder and dental bracket water at the ratio of 1.0∶1.5, 50% sodium hydroxide solution were used to corrode the lung tissue. The intact, moderately elastic, clean and smooth respiratory cast specimens could be obtained, and bronchial trees could be clearly seen. Sodium hydroxide solution did not need to be equipped with other solutions, and the substance was not volatile. It is the most convenient and safe method among the three corrosion methods.

Key words: rabbit, tracheal bronchus, specimen, casting mold, corrosion method

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