犬脂肪间充质干细胞静脉移植临床安全性研究

doi:10.19640/j.cnki.jtau.2023.06.010

天津农学院学报 ›› 2023, Vol. 30 ›› Issue (6): 62-68.doi: 10.19640/j.cnki.jtau.2023.06.010

犬脂肪间充质干细胞静脉移植临床安全性研究

罗家明¹, 葛怀娜², 白春娜², 葛秀国^{1, 通信作者}

1.天津农学院动物科学与动物医学学院,天津 300392;
2.百欧派（天津）生物技术有限公司,天津 300350

收稿日期:2023-01-01 出版日期:2023-12-31 发布日期:2024-02-05
通讯作者: 葛秀国（1977—）,男,副教授,博士,主要从事动物干细胞与外科学研究。E-mail：xiuguoge@163.com。
作者简介:罗家明（2001—）,男,本科在读,主要从事动物医学研究。E-mail：luojiaming2001@163.com。
基金资助:
天津农学院横向合作项目（TNHXKJ2021076）

Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation

Luo Jiaming¹, Ge Huaina², Bai Chunna², Ge Xiuguo^{1, Corresponding Author}

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Baioupai Tianjin Biotechnology Co., Ltd., Tianjin 300350, China

Received:2023-01-01 Online:2023-12-31 Published:2024-02-05

摘要/Abstract

摘要： 为了验证犬脂肪间充质干细胞（adipose-derived stem cells,ASCs）临床应用的安全性,本试验取第4代犬ASCs,显微镜下观察细胞形态,并进行成脂、成骨及成软骨诱导分化和细胞表面标志蛋白分析。结果显示ASCs呈纺锤体状贴壁生长,形态均匀一致,体外ASCs可分化为脂肪细胞、骨细胞、软骨细胞。犬ASCs表达标记蛋白CD29、CD44、CD105和CD166,不表达CD34和CD45,符合典型的脂肪间充质干细胞特征。犬ASCs以1×10⁶/kg进行静脉移植,速度10 mL/（kg·h）,注射过程中未见任何不良反应。长期观察3个月,未见明显相关并发症。与移植前相比,血液检查发现白细胞数、中性粒细胞数、单核细胞数、嗜酸性粒细胞数、红细胞数、血红蛋白和血小板数等均无显著差异（P>0.05）;血清生化检查发现白蛋白、ALT、AKP等肝指标差异不显著（P>0.05）,肾指标肌酐、血清尿素、血磷和血钙差异不显著（P>0.05）,胰腺相关指标血糖和血清淀粉酶均无显著差异（P>0.05）,心肌损伤指标肌酸激酶和血脂指标胆固醇,在细胞移植前后也无显著差异（P>0.05）。本试验结果表明了犬ASCs应用的临床安全性,为细胞移植治疗奠定了基础。

关键词: 犬, 脂肪间充质干细胞, 移植, 安全性

Abstract: In order to verify the safety of clinical application of canine adipose derived stem cells （ASCs）, the fourth generation of canine ASCs were selected in this experiment, and the cell morphology was observed under the microscope, and adipogenesis, osteogenesis and chondrogenesis were induced and differentiated, and cell surface marker proteins were analyzed. The results showed that ASCs grew as spindle-like adherent cells with uniform morphology. ASCs could differentiate into adipocytes, osteocytes and chondrocytes in vitro. Canine ASCs express marker proteins CD29, CD44, CD105 and CD166, but do not express CD34 and CD45, which conforms to the typical characteristics of adipose mesenchymal stem cells. Canine ASCs with 1×10⁶/kg vein transplantation was performed at a speed of 10 mL/（kg·h）. No adverse reaction was found during the injection. Long term observation for 3 months showed no obvious complications. Compared with that before transplantation, the number of white blood cells, neutrophils, monocytes, eosinophils, red blood cells, hemoglobin and platelets showed no significant difference（P>0.05）; Serum biochemical examination showed that there were no significant differences in liver indicators such as albumin, ALT, AKP（P>0.05）, renal indicators such as creatinine, serum urea, blood phosphorus and blood calcium（P>0.05）, pancreatic related indicators such as blood glucose and serum amylase（P>0.05）, myocardial injury indicators such as creatine kinase and blood lipid indicators such as cholesterol before and after cell transplantation（P>0.05）. The results of this study showed the clinical safety of ASCs in dogs and laid a foundation for cell transplantation therapy.

Key words: canine, adipose mesenchymal stem cells, transplantation, safety

中图分类号:

S854.5
Q28

罗家明, 葛怀娜, 白春娜, 葛秀国. 犬脂肪间充质干细胞静脉移植临床安全性研究[J]. 天津农学院学报, 2023, 30(6): 62-68.

Luo Jiaming, Ge Huaina, Bai Chunna, Ge Xiuguo. Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation[J]. Journal of Tianjin Agricultural University, 2023, 30(6): 62-68.

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http://xuebao.tjau.edu.cn/CN/Y2023/V30/I6/62

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犬脂肪间充质干细胞静脉移植临床安全性研究

Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation

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