拟南芥叶片衰老突变体ein3、max2延迟营养生长时相转变

doi:10.19640/j.cnki.jtau.2020.04.002

天津农学院学报 ›› 2020, Vol. 27 ›› Issue (4): 7-11.doi: 10.19640/j.cnki.jtau.2020.04.002

拟南芥叶片衰老突变体ein3、max2延迟营养生长时相转变

尹晟, 傅钰, 龙鸿^通信作者

天津农学院园艺园林学院,天津 300392

收稿日期:2020-04-02 出版日期:2020-12-31 发布日期:2020-12-25
通讯作者: 龙鸿（1964—）,男,教授,博士,主要从事果树遗传、发育生物学的研究。E-mail：longhong@tjau.edu.cn。
作者简介:尹晟（1994—）,男,硕士在读,主要从事果树遗传方面的研究。E-mail：554182342@qq.com。
基金资助:
天津农学院研究生培养质量提升项目（101018）

The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana

Yin Sheng, Fu Yu, Long Hong^{Corresponding Author}

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-04-02 Online:2020-12-31 Published:2020-12-25

摘要/Abstract

摘要： 高等植物营养生长阶段根据能否诱导开花分为前期的幼龄期和后期的成熟期,其转变过程称为营养生长时相转变。营养生长时相转变和营养生长到生殖生长的转变,都是植物生长发育过程中的重要生理过程,均与植物自身发育的时间进程相关,受到一些共同的基因调控,而叶片衰老相关基因在营养生长时相转变中的调控作用仍不明确。本文研究了叶片衰老相关基因EIN3和MAX2的突变体营养生长时相转变特点。莲座叶叶形、远轴面表皮毛产生时间、叶基角特征等形态观测表明,拟南芥突变体ein3、max2延迟了营养生长时相转变。对ein3、max2植株生长发育不同阶段的miR156、SPL3表达水平检测表明,miR156和SPL3的表达量分别呈下降和上升的趋势,导致营养生长时相转变延迟。突变体由于叶片衰老进程受到影响,其发育迟缓,暗示 EIN3和MAX2可能通过miR156及SPL3表达水平来调控营养生长时相转变。

关键词: 营养生长时相转变, 叶片衰老基因, ein3, max2, 拟南芥

Abstract: In higher plants, vegetative phase change refers to the transition from juvenile to adult phase, and after that plants can blossom. The vegetative phase change and the transition from vegetative growth to reproductive growth are important physiological processes in plant growth and development. Both processes are related to the timespan of plant development, sharing some regulated genes. However, regulation of leaf senescence related genes during this transition remains unknown. This study investigated the characteristics of vegetative phase change via leaf senescence related mutants ein3 and max2. Morphological observations of rosette leaf shape, onset of abaxial trichomes, blade base angles showed that vegetative phase change was delayed in ein3 and max2. Expression levels of miR156 and SPL3 at different stages of ein3 and max2 plant development showed down-regulated and up-regulated trend, leading to a delayed vegetative phase change. Compared with wild types, mutant plants developed slowly, due to the ongoing leaf senescence. The data suggested that EIN3 and MAX2 may play a role in regulating plant vegetative phase change through expression levels of miR156 and SPL3．

Key words: vegetative phase change, leaf senescence gene, ein3, max2, Arabidopsis thaliana

中图分类号:

Q945.4

尹晟, 傅钰, 龙鸿. 拟南芥叶片衰老突变体ein3、max2延迟营养生长时相转变[J]. 天津农学院学报, 2020, 27(4): 7-11.

Yin Sheng, Fu Yu, Long Hong. The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana[J]. Journal of Tianjin Agricultural University, 2020, 27(4): 7-11.

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拟南芥叶片衰老突变体ein3、max2延迟营养生长时相转变

The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana

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