天津农学院学报 ›› 2021, Vol. 28 ›› Issue (4): 1-5.doi: 10.19640/j.cnki.jtau.2021.04.001

• 研究与简报 •    下一篇

盐胁迫对小果黑核桃幼苗外部形态和生理变化的影响

刘昊1, 王倩2, 徐惠敏3, 贺佳琪2, 李爱2, 高英2,通信作者   

  1. 1.天津市农业科学院 果树研究所,天津 300384;
    2.天津农学院 园艺园林学院,天津 300392;
    3.河南省洛宁县林业局,河南 洛宁 471700
  • 收稿日期:2020-09-23 出版日期:2021-12-31 发布日期:2022-01-05
  • 通讯作者: 高英(1982—),女,讲师,博士,主要从事果树栽培育种方面的研究。E-mail:gying@tjau.edu.cn。
  • 作者简介:刘昊(1983—),男,助理研究员,博士,主要从事林果栽培及育种方面的研究。E-mail:glkl@163.com。
  • 基金资助:
    天津农学院科学研究发展基金计划资助项目(2016NYB07); 国家自然科学基金项目(31800572); 天津市林果现代农业产业技术体系项目(ITTHRS2021000)

Effects of salt stress on osmotic regulator and antioxidase activities in Juglans microcarpa seedlings

Liu Hao1, Wang Qian2, Xu Huimin3, He Jiaqi2, Li Ai2, Gao Ying2,Corresponding Author   

  1. 1. The Research Institate of Pomology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China;
    2. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
    3. Luoning County Forestry Bureau, Luoning 471700, Henan Province, China
  • Received:2020-09-23 Online:2021-12-31 Published:2022-01-05

摘要: 为了解核桃砧木耐盐性以便为耐盐核桃砧木的筛选提供依据,以小果黑核桃幼苗为试材,采用NaCl溶液对其进行盐胁迫处理,观察幼苗受胁迫后的生长状况,并测定核桃幼苗处理后不同天数的相关生理指标。结果表明:核桃幼苗受盐害后,叶片失水萎蔫,叶片变黄;植株在高浓度长时间盐胁迫下叶片变黑直至植株死亡。随着盐浓度的升高和胁迫时间的增加,POD和SOD活性先升高后降低、游离脯氨酸大量积累、可溶性蛋白含量变化趋势为先增加后减少。研究认为小果黑核桃在100 mmol/L NaCl胁迫时表现出较强的耐受性。

关键词: 小果黑核桃, 盐胁迫, 抗氧化酶, 渗透调节物质

Abstract: In order to research the salt tolerance of walnut rootstocks, so as to provide scientific basis for screening salt-tolerant walnut rootstocks, the seedlings of Juglans microcarpa were treated under salt stress with NaCl and the growth situation of seedlings were observed. The physiological indexes of Juglans microcarpa seedlings were also measured in different days after treatment. The results showed that the walnut leaves were wilted and the leaves became yellow. The leaves become dark until the plants die under high-concentration and long-term salt stress. With the increase of salt concentration and stress time, the activity of POD and SOD increased first and then decreased, the free proline accumulated, and the change trend of soluble protein content was increasing first and then decreasing. It was found that the seedlings of Juglans microcarpa showed tolerance of salt stress with 100 mmol/L NaCl.

Key words: Juglans microcarpa, salt tolerance, antioxidant enzymes, osmoregulation substance

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