VBNC状态鸡白痢沙门氏菌快速诱导条件筛选

Journal of Tianjin Agricultural University ›› 2013, Vol. 20 ›› Issue (3): 37-41.

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Selection of VBNC State Rapid Induction Condition of Salmonella Pullorum

HU Ye¹, MA Ji-fei¹, ZHAO Rui-li¹, BIAN Yan², YANG Jiu-qiang³

1. Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China;
2. Jinghai Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 301600, China;
3. Jinnan Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 300350, China

Received:2013-04-21 Published:2019-10-21

Abstract

Abstract: In order to establish the rapid induction condition of VBNC state Salmonella pulorum, the induction at the low temperature of 4 ℃ was selected as the control group, and on this basis, the experiment was conducted through adding different concentrations of Cu²⁺ and glacial acetic acid as the rapid induction condition. The results show that adding 2~4 mM Cu²⁺ could obviously promote Salmonella into VBNC state at 4 ℃ low temperature, and compared with the traditional induction condition, the induction time could be advanced 15 d averagely. The Salmonella entering into the VBNC state was identified by 16S rRNA test, and the results proved that the stains were Salmonella of VBNC state.

Key words: viable but non-culturable state, Salmonella, 16S rRNA, Cu²⁺

CLC Number:

S852.614

HU Ye, MA Ji-fei, ZHAO Rui-li, BIAN Yan, YANG Jiu-qiang. Selection of VBNC State Rapid Induction Condition of Salmonella Pullorum[J]. Journal of Tianjin Agricultural University, 2013, 20(3): 37-41.

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Selection of VBNC State Rapid Induction Condition of Salmonella Pullorum

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