Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (3): 12-17.doi: 10.19640/j.cnki.jtau.2025.03.003

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Flavour analysis and ecological evaluation of free amino acids in sexually precocious Eriocheir sinensis

Miao Hongyeb, Liu Yananb, Gao Jinweia,b, Jia Xuyinga,b, Zhou Wenlia,b, Zhang Dana,b,Corresponding Author   

  1. Tianjin Agricultural University, a. Key Laboratory of Smart Breeding(Co-construction by Ministry and Province), b. Tianjin Key Lab of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin 300392, China
  • Received:2025-04-13 Online:2025-06-30 Published:2025-07-02

Abstract: In the present study, ultra performance liquid chromatography-mass spectrometry(UPLC-MS)combined with multivariate statistical methods was used to systematically analyze the differences in free amino acid compositions(FAA)of muscle and hepatopancreas between sexually precocious individuals and normal individuals of Eriocheir sinensis, as well as the comprehensive ecological evaluatation. The results showed that 20 FAA were detected in muscle and hepatopancreas tissues respectively, and the total FAAs in hepatopancreas of precocious crab[(22 210.39±11.59)μg/g] were significantly higher than those of normal crab[(17 169.87±11.87)μg/g](P<0.05), of which the total contents of sweet amino acids in the muscle and hepatopancreas of precocious crab were significantly higher than those of normal crab(P<0.05). The TAV of fresh and sweet amino acids in precocious E. sinensis was significantly higher than those of normal crabs(P<0.05), and the flavor showed fresh and sweet characteristics, but its comprehensive quality was limited by the potential negative effect of bitter amino acids. It was suggested that precocious puberty may significantly affect the FAA composition of E. sinensis through the amino acid metabolic pathway, and FAA metabolism in the hepatopancreas was more sensitive to precocious puberty. The present study provides a theoretical basis for the study of the flavor quality of early-maturing E. sinensis and its application in the industry through the comprehensive ecological analysis of flavor.

Key words: Eriocheir sinensis, muscle, hepatopancreas, free amino acids

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