MSTN基因敲除促进牛骨骼肌脂肪代谢

doi:10.19640/j.cnki.jtau.2022.03.009

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (3): 47-51.doi: 10.19640/j.cnki.jtau.2022.03.009

• Researches and Scientific Notes • Previous Articles Next Articles

Study on fat metabolism of bovine skeletal muscle by MSTN gene

Sheng Hui, Guo Yiwen, Zhang Linlin, Li Xin, Din Xiangbin, Guo Hong^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-09-16 Online:2022-09-30 Published:2022-10-10

Abstract

Abstract: To investigate the effect of myostatin（MSTN）on skeletal muscle fat metabolism, MSTN^-/-Mongolian cattle （MG.ZJY）and wild Mongolian cattle（MG.DZ）, MSTN^-/- Luxi cattle（LX.ZJY）and wild Luxi cattle（LX.DZ）and MSTN^-/- Mongolian Black Cattle（HN.ZJY）and wild Mongolian black cattle（HN.DZ）leg gluteal muscle tissue as a sample, quantitative proteomics and phosphorus Acidified proteomics technology were used to screen differential proteins and differentially phosphorylated proteins. These differential proteins and phosphorylated proteins were statistically analyzed by online bioinformatics analysis software and website, and triglyceride content in various tissues were detected. The results showed that the identified multiple differential proteins were all related to fat metabolism, and these differential proteins were mainly enriched in fatty acid degradation, fatty acid metabolism and PPAR signaling pathways. Tissue triglyceride assay showed that the triglyceride content in muscle tissue of MSTN^-/- cattle was significantly decreased compared with that of wild cattle. The results of this study showed that knockout of MSTN gene could promote fatty acid metabolism in bovine skeletal muscle. The results lay a foundation for further research on the effect of MSTN gene on skeletal muscle metabolic mechanism.

Key words: MSTN, skeletal muscle, proteomics, fatty acid metabolism

CLC Number:

S813.3

Sheng Hui, Guo Yiwen, Zhang Linlin, Li Xin, Din Xiangbin, Guo Hong. Study on fat metabolism of bovine skeletal muscle by MSTN gene[J]. Journal of Tianjin Agricultural University, 2022, 29(3): 47-51.

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References

[1] LEE S J,MCPHERRON A C.Myostatin and the control of skeletal muscle mass[J]. Current Opinion in Genetics & Development,1999,9(5):604-607.
[2] SHARMA M,LANGLEY B,BASS J,et al.Myostatin in muscle growth and repair[J]. Exercise and Sport Sciences Reviews,2001,29(4):155-158.
[3] 曹婷,周汉林,荀文娟,等. MSTN基因对猪骨骼肌发育调控的作用及其研究进展[J]. 基因组学与应用生物学,2017,36(4):1511-1517.
[4] FAN X,GAUR U,SUN L,et al.The Growth Differentiation Factor 11(GDF₁₁)and Myostatin (MSTN)in tissue specific aging[J]. Mech Ageing Dev,2017,164:108-112.
[5] PIRRUCCELLO-STRAUB M,JACKSON J,WAWERSIK S,et al.Blocking extracellular activation of myostatin as a strategy for treating muscle wasting[J]. Scientific Reports,2018,8(1):2292.
[6] MANFREDI L H,PAULA-GOMES S,ZANON N M,et al.Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents[J]. Brazilian Journal of Medical and Biological Research,2017,50(12):e6733.
[7] SABROUT E I,AGGAG S.Association of Melanocortin(MC4R)and Myostatin(MSTN)genes with carcass quality in rabbit[J]. Meat Science,2018,137:67-70.
[8] MOSHER D S,QUIGNON P,BUSTAMANTE C D,et al.A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs[J]. PLoS Genetics,2007,3(5):e79.
[9] WANG K,OUYANG H,XIE Z,et al.Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system[J]. Scientific Reports,2015,5:16623.
[10] DENG B,DING Y,WEN J H,et al.Expression analysis of myostatin and its receptor during the growth of adipose and muscle tissues in Meishan pig[J]. Indian J Anim Sci,2012,82:44-47.
[11] 朱晓蕾,李晓南. 姜黄素对脂肪组织功能调控作用的研究进展[J]. 中国食物与营养,2020,26(7):46-50.
[12] VIRTANEN K A.BAT thermogenesis: Linking shivering to exercise[J]. Cell Metab,2014,19(3):352-354.
[13] COELHO M,OLIVEIRA T,FERNANDES R.Biochemistry of adipose tissue: An endocrine organ[J]. Arch Med Sci,2013,9(2):191-200.
[14] BAATI N,FEILLET-COUDRAY C,FOURET G,et al.Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles[J]. Biochim Biophys Acta Mol Cell Biol Lipids,2017,1862(10):1044-1055.
[15] ZHANG C,MCFARLANE C,LOKIREDDY S,et al.Inhibition of myostatin protects against diet-induced obesity by enhancing fatty acid oxidation and promoting a brown adipose phenotype in mice[J]. Diabetologia,2012,55(1):183-193.
[16] 高洋. MSTN基因敲除抑制小鼠肌肉三羧酸循环和氧化磷酸化[D]. 呼和浩特:内蒙古大学,2017.
[17] YANG S P,LI X,LIU X F,et al.Parallel comparative proteomics and phosphoproteomics reveal that cattle myostatin regulates phosphorylation of key enzymes in glycogen metabolism and glycolysis pathway[J]. Oncotarget,2018,9(13):11352-11370.
[18] XIN X B,YANG S P,LI X,et al.Proteomics insights into the effects of MSTN on muscle glucose and lipid metabolism in genetically edited cattle[J]. Gen Comp Endocrinol,2020,291:113237.

Study on fat metabolism of bovine skeletal muscle by MSTN gene

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