金钗石斛组培苗壮苗培养研究

doi:10.19640/j.cnki.jtau.2024.02.003

Journal of Tianjin Agricultural University ›› 2024, Vol. 31 ›› Issue (2): 12-17.doi: 10.19640/j.cnki.jtau.2024.02.003

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Optimization of tissue culture and rapid propagation system of dendrobium nobile

Xue Yuan, Li Deping, Yi Yuanhui, Yang Dandan, Niu Weiyuan, Liu Yanjun^{Corresponding Author}

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2023-01-06 Online:2024-04-30 Published:2024-05-22

Abstract

Abstract: In order to study the factors that affect the rapid growth of Dendrobium nobile tissue culture seedlings, this experiment took Dendrobium nobile tissue culture seedlings as the experimental material, and set up an orthogonal test at the three levels of auxin NAA, hydrolyzed milk protein, organic components of MS medium, and activated carbon to observe the growth performance of Dendrobium nobile tissue culture seedlings, so as to determine the best medium combination. The results showed that the combination of 0.10 g/L activated carbon+0.50 mg/L NAA+0.05 g/L hydrolyzed milk protein+1 fold organic component had the best effect on the growth of tissue culture seedlings; and NAA can significantly increase the growth height and coarseness of tissue culture seedlings. This study lays a foundation for the study of tissue culture and rapid propagation of Dendrobium nobile.

Key words: Dendrobium nobile, basic medium, strong plantlets in tissue culture, orthogonal test

CLC Number:

Xue Yuan, Li Deping, Yi Yuanhui, Yang Dandan, Niu Weiyuan, Liu Yanjun. Optimization of tissue culture and rapid propagation system of dendrobium nobile[J]. Journal of Tianjin Agricultural University, 2024, 31(2): 12-17.

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Optimization of tissue culture and rapid propagation system of dendrobium nobile

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