一株野生古巴栓孔菌生物学特性的研究

doi:10.19640/j.cnki.jtau.2021.02.006

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (2): 31-35.doi: 10.19640/j.cnki.jtau.2021.02.006

• Researches and Scientific Notes • Previous Articles Next Articles

Studies on the biological characteristics of a wild Trametes Cubensis

Shi Weili, Ban Litong^*, Huang Liang, Yang Hongpeng, Sun Ning, Wang Yu, Li Yiting, Zhang Yi

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-07-02 Published:2021-07-26

Abstract

Abstract: In this experiment, a single factor and orthogonal test methods were used to study the optimal culture conditions of a wild Trametes cubensis hyphae. The single factor test results showed that the optimal carbon source and nitrogen source for the mycelium of Trametes cubensis were glucose and yeast extract powder, and the optimal addition amount of carbon source and nitrogen source were 5 g/L, 3 g/L. The optimum culture temperature was 35 ℃, and the initial pH of the medium had no significant effect on the growth of the hyphae of Trametes Cubensis. Orthogonal test results showed that the order of the effects on the growth of the mycelium of Trametes Cubensis was: carbon source>temperature>nitrogen source. The results of orthogonal test showed that the optimum medium was glucose 15 g/L, yeast extract 3 g/L, magnesium sulfate 0.5 g/L, monopotassium phosphate 1 g/L, VB₁ 0.01 g/L, Agar 18 g/L, natural pH, culture temperature 36 ℃.

Key words: Trametes cubensis, mycelial growth, temperature, carbon and nitrogen sources, orthogonal test

CLC Number:

S646

Shi Weili, Ban Litong, Huang Liang, Yang Hongpeng, Sun Ning, Wang Yu, Li Yiting, Zhang Yi. Studies on the biological characteristics of a wild Trametes Cubensis[J]. Journal of Tianjin Agricultural University, 2021, 28(2): 31-35.

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References

[1] 戴玉成,周丽伟,杨祝良,等. 中国食用菌名录[J]. 菌物学报,2010,29(1):1-21.
[2] 李臻. 中国古代菌蕈文化中关于灵芝的记载[J]. 中国食用菌,2020,39(3):129-131,134.
[3] 秦淼. 野生食用菌资源利用现状及对策探究[J]. 南方农业,2019,13(Z1):117-118.
[4] 万涛. 野生食用菌资源利用现状及发展前景[J]. 河南农业,2019(10):41-42.
[5] 袁江玲,雒婉霞,徐晓辉,等. 一株新疆罕见野生大型真菌的形态及分子生物学鉴定[J]. 疾病预防控制通报,2020,35(3):14-17.
[6] 晚安琪,蔺晓云,黄亮,等. 一株侧耳属野生菌的分离鉴定及初步驯化栽培[J]. 天津农学院学报,2020,27(2):28-30.
[7] 邱志伟,陈有忠,于奥安,等. 枣树根部内生细菌的分离及对3种枣病原真菌拮抗活性研究[J]. 天津农学院学报,2019,26(3):12-16.
[8] 孙晓莉,高建,潘乐欣,等. 猪大肠杆菌性腹泻病的病原分离鉴定及药敏试验[J]. 天津农学院学报,2019,26(2):57-60.
[9] 郑义. 东方栓孔菌多糖的分离纯化、结构解析与生物活性研究[D]. 徐州:中国矿业大学,2019.
[10] 周凌云,鲁斌,孙玉. 东方栓孔菌多糖对小鼠的降血糖和降血脂作用[J]. 南方医科大学学报,2020,40(8):1127-1133.
[11] 郝金斌,孟国良,杨军,等. 奶油栓孔菌子实体多糖的理化性质、抗氧化活性与保肝作用[J]. 菌物学报,2020,39(12):2355-2368.
[12] 魏铁铮,王科,于晓丹,等. 中国大型担子菌受威胁现状评估[J]. 生物多样性,2020,28(1):41-53.
[13] 卯晓岚. 我国野生食用菌的驯化选育近况[J]. 北京农业,2006(3):19-20.
[14] 杨慧,尚晓冬,王瑞霞,等. 蒸馏水与液氮保藏金针菇菌种特性的比较[J]. 食用菌学报,2010,17(4):23-25.
[15] 曲娜,刘迪,王皓,等. 长白山野生珊瑚状猴头菌菌丝最佳培养基配方研究[J]. 中国食用菌,2020,39(4):17-19.
[16] 张琦,王航,刘琨,等. 干巴菌菌丝生物学特性及生长条件研究[J]. 贵州农业科学,2019,47(5):55-60.
[17] 孙厚静,谌金吾,闫静,等. 灰树花菌丝生长最佳培养基碳氮比研究[J]. 食用菌,2019,41(5):18-20.
[18] 杨焕玲,查磊,赵妍,等. 新型香菇母种培养基的筛选[J]. 江苏农业科学,2019,47(3):124-126.
[19] 田雪梅,宋爱荣. 温度对樟芝菌丝体生长的影响试验[J]. 中国食用菌,2005,24(5):51-52.
[20] 毕旺华,田雪梅,孙效乐,等. 毛栓孔菌菌丝体生长营养条件的优化研究[J]. 青岛农业大学学报(自然科学版),2012,29(1):51-54.
[21] KUDAHETTIGE R L,HOLMGREN M,IMERZEEL P,et al.Characterization of bioethanol production from hexoses and xylose by the white rot fungus Trametes versicolor[J]. Bioenerg Res,2012,5(2):277-285.
[22] SHAKHOVA N V,GOLENKINA S A,STEPANOV A E V,et al. Effect of submerged cultivation conditions and inducers on biosynthesis of extracellular laccase by a Trametes versicolor 1666 strain[J]. Appl Biochem Micro+,2011,47(9):808-816.
[23] 周永斌,张志军,刘连强,等. 白灰树花菌丝体最适营养生长条件的研究[J]. 中国食用菌,2010,29(6):26-27.
[24] 孙康迪,柳青云,方咏青,等. 黄芪多糖、灵芝多糖和转移因子对犬瘟热免疫效果的影响[J]. 天津农学院学报,2020,27(1):58-60.

Studies on the biological characteristics of a wild Trametes Cubensis

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