马铃薯脱毒苗组培快繁影响因子的优化

doi:10.19640/j.cnki.jtau.2026.02.004

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (2): 17-22.doi: 10.19640/j.cnki.jtau.2026.02.004

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Optimization of influencing factors on tissue culture and rapid propagation of virus-free potato seedlings

Wang Xiaomeng¹, Lü Siyao¹, Sun Ning^{1,Corresponding Author}, Qi Xin^{2,Corresponding Author}

1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Academy of Agriculture Sciences, Tianjin 300192, China

Received:2024-10-12 Online:2026-04-30 Published:2026-05-06

Abstract

Abstract: Using potato cultivar ‘Favorita’ as the material, the effects of media type, exogenous hormone, carbon source, activated carbon and water quality on tissue culture and rapid propagation of virus-free potato seedlings were studied. The results showed that the growth of virus-free potato seedlings on MS medium was superior to that on B5 and N6 media. However, the number of leaves per plant and number of new shoots on B5 medium were significantly higher than those on MS. The addition 1-2 g/L activated carbon was beneficial to the growth and proliferation of virus-free potato seedlings, with the average number of leaves and new shoots being 45.7% and 47.7% higher than those of the control group without activated carbon respectively. The addition of 0.5 mg/L 6-BA and 0.2 mg/L NAA could effectively promote the growth of virus-free potato seedlings. Replacing sucrose in MS medium with 20-25 g/L white sugar as the carbon source, and using tap water in the preparation of medium, not only improved the quality and propagation efficiency of virus-free seedlings, but also effectively reduced the production costs.

Key words: potato, virus-free seedlings, tissue culture and rapid propagation, growth, proliferation

CLC Number:

S532
Q813.1

Wang Xiaomeng, Lü Siyao, Sun Ning, Qi Xin. Optimization of influencing factors on tissue culture and rapid propagation of virus-free potato seedlings[J]. Journal of Tianjin Agricultural University, 2026, 33(2): 17-22.

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Optimization of influencing factors on tissue culture and rapid propagation of virus-free potato seedlings

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