产朊假丝酵母富硒条件的优化及发酵罐培养

doi:10.19640/j.cnki.jtau.2022.04.006

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (4): 26-30.doi: 10.19640/j.cnki.jtau.2022.04.006

产朊假丝酵母富硒条件的优化及发酵罐培养

马楚楚, 陈帅君^通信作者, 赵宝顶, 唐伦霞, 童应凯, 边嘉宾

天津农学院农学与资源环境学院,天津 300392

收稿日期:2021-11-10 出版日期:2022-12-30 发布日期:2023-01-03
通讯作者: 陈帅君（1982—）,女,讲师,硕士,研究方向：生物技术育种。E-mail：86895955@qq.com。
作者简介:马楚楚（1995—）,男,本科在读,研究方向：微生物发酵。E-mail：18222328979@qq.com。
基金资助:
天津市大学生创新创业训练计划项目（201810061122）; 天津市一二三产业融合发展科技示范工程项目（18ZXYENC00140）; 天津市农村科技帮扶工程项目（18ZXBFNC00330）

Optimization of selenium enrichment condition and culture of Candida utilis in fermenter

Ma Chuchu, Chen Shuaijun^{Corresponding Author}, Zhao Baoding, Tang Lunxia, Tong Yingkai, Bian Jiabin

College of Agronomy and Resources Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2021-11-10 Online:2022-12-30 Published:2023-01-03

摘要/Abstract

摘要： 用富硒产朊假丝酵母制备饲料添加剂饲喂家畜家禽可获得富硒农产品。为获取富硒产朊假丝酵母,以产朊假丝酵母为出发菌株,优化摇瓶培养条件（摇瓶装液量、接种量、亚硒酸钠添加量、添加时间和培养液初始pH）,通过L₉（3⁴）正交试验,获得最佳发酵工艺,即：装液量为35 mL,初始pH为6.5,接种量8%,培养时间为12 h,亚硒酸钠添加量为15 μg/mL,此时最终发酵液中总有机硒含量最高,为9 792.732 μg/L,菌体有机硒含量为1 400.362 μg/g。用获得的高生物量富硒产朊假丝酵母制作饲料添加剂,通过补料培养,获得生物量为19.128 g/L,发酵液中总有机硒量为 10 089 μg/L。

关键词: 产朊假丝酵母, 富硒, 发酵罐, 条件优化

Abstract: To obtain selenium-enriched Candida utilis to prepare feed additives to feed livestock and poultry, and obtain selenium-enriched agricultural products, C. utilis was used as the starting strain to optimize the shake flask culture conditions, including the volume of shake flask, the inoculation volume, the amount and time of sodium selenite addition, and the initial pH of the culture fluid. Then the L₉（3⁴）orthogonal test was done to obtain the optimum fermentation process when liquid volume was 35 mL, the initial pH was 6.5, and the inoculation volume was 8%. When sodium selenite was added at 12 h at 15 μg/mL, the total organic selenium content in the final fermentation broth was the highest at 9 792.732 μg/L. The content was 1 400.362 μg/mL. The fermentor was then cultivated to obtain high biomass selenium-rich C. utilis to make feed additives. Through feed culture, the biomass was 19.128 g/L, and the total organic selenium in the fermentation broth was 10 089 μg/L.

Key words: Candida utilis, selenium enrichment, fermentation tank, optimization of condition

中图分类号:

马楚楚, 陈帅君, 赵宝顶, 唐伦霞, 童应凯, 边嘉宾. 产朊假丝酵母富硒条件的优化及发酵罐培养[J]. 天津农学院学报, 2022, 29(4): 26-30.

Ma Chuchu, Chen Shuaijun, Zhao Baoding, Tang Lunxia, Tong Yingkai, Bian Jiabin. Optimization of selenium enrichment condition and culture of Candida utilis in fermenter[J]. Journal of Tianjin Agricultural University, 2022, 29(4): 26-30.

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产朊假丝酵母富硒条件的优化及发酵罐培养

Optimization of selenium enrichment condition and culture of Candida utilis in fermenter

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