天津农学院学报 ›› 2020, Vol. 27 ›› Issue (3): 49-52.doi: 10.19640/j.cnki.jtau.2020.03.011

• 研究与简报 • 上一篇    下一篇

富硒乳酸菌对小鼠生长、血液生化指标、肠道菌群和细胞因子的影响

傅春妮, 周磊, 王欢欢, 李元辉, 秦顺义通信作者   

  1. 天津农学院 动物科学与动物医学学院,天津 300384
  • 收稿日期:2019-06-26 出版日期:2020-10-10 发布日期:2020-10-10
  • 通讯作者: 秦顺义(1979-),男,副教授,博士,研究方向为动物营养代谢病与中毒病。E-mail:qinshunyi@163.com。
  • 作者简介:傅春妮(1995-),女,硕士在读,研究方向为动物生长调控与生理学。E-mail:fuchunni6@163.com。
  • 基金资助:
    天津市高校“中青年骨干创新人才培养计划”项目(无编号); 天津市生猪产业技术体系创新团队细菌病岗位专家项目(ITTPRS 2018004)

Effects of selenium-enriched lactic acid bacteria on growth, blood biochemical indexes, intestinal flora and cytokines in mice

FU Chun-ni, ZHOU Lei, WANG Huan-huan, LI Yuan-hui, QIN Shun-yiCorresponding Author   

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2019-06-26 Online:2020-10-10 Published:2020-10-10

摘要: 为研究富硒乳酸菌对小鼠生长、血液生化指标、肠道菌群和细胞因子的影响,选取清洁级昆明小鼠32只,随机分为2组。对照组:饲喂基础日粮,每日提供正常饮水;Se组:饲喂基础日粮,并每日提供12 h添加富硒乳酸菌的饮水(活菌数1.0×1011 cfu/L,硒含量0.12 mg/L)。45 d后测量小鼠的生长性能、脏器指数、血液生化指标、肠道菌群和血清细胞因子。结果表明:Se组与对照组小鼠的心脏、肝脏、脾脏、肺脏和肾脏指数均无显著差异(P>0.05);Se组与对照组小鼠的采食量、料重比和增重均无显著差异(P>0.05);Se组与对照组小鼠的总蛋白、白蛋白、球蛋白、肌酐和尿素氮含量均无显著差异(P>0.05);与对照组相比,Se组小鼠的肠球菌数显著降低(P<0.05),乳酸杆菌数显著增加(P<0.05),大肠杆菌数和双歧杆菌数均无显著差异(P>0.05);Se组与对照组小鼠的IL-2、IL-4、IL-8、IFN-g水平无显著差异(P>0.05)。综上所述,本试验结果提示:富硒乳酸菌对小鼠的生长、血液生化指标及IL-2、IL-4、IL-8、IFN-g水平无影响;富硒乳酸菌能够增加小鼠肠道乳酸杆菌数量、降低肠球菌数量。

关键词: 富硒乳酸菌, 生化指标, 肠道菌群, 细胞因子

Abstract: In order to study the effects of selenium-enriched lactic acid bacteria on growth, blood biochemical indexes, intestinal flora and cytokines in mice, in this experiment, 32 Kunming mice of clean grade were selected, randomly divided into two groups. Control group: the control group was fed with basic diet and provided with normal drinking water every day. Group Se: basic diet was fed and drinking water supplemented with selenium-rich lactic acid bacteria for 12 hours a day.(The number of living bacteria was 1.0×1011cfu/L, selenium content was 0.12 mg/L). After 45 days, the growth performance, organ index, blood biochemical index, intestinal flora and serum cytokines were measured. The results showed that there was no significant difference in heart, liver, spleen, lung and kidney index between Se group and control group(P>0.05); There were no significant differences in feed intake, feed weight ratio and weight gain between Se group and control group(P>0.05); There were no significant differences in total protein, albumin, globulin, creatinine and urea nitrogen content between Se group and control group(P>0.05); Compared with the control group, the number of enterococci in Se group decreased significantly(P<0.05)and the number of Bacillus acidilactici increased significantly(P<0.05), but there was no significant difference in the number of E.coli and bifidobacterium between Se group and control group(P>0.05). There was no significant difference in IL-2, IL-4, IL-8, IFN-g level between Se group and control group(P>0.05). To sum up, the results showed that selenium-enriched lactic acid bacteria had no effect on the growth, blood biochemical indexes and IL-2, IL-4, IL-8, IFN-g level of mice; Selenium-enriched lactic acid bacteria can increase the number of lactobacterium in the intestinal tract of mice and decrease the number of enterococcus.

Key words: Se-rich lactic acid bacteria, biochemical index, intestinal flora, cytokine

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