富硒乳酸菌对肉鸡新城疫病毒抗体影响的研究

doi:10.19640/j.cnki.jtau.2018.02.009

天津农学院学报 ›› 2018, Vol. 25 ›› Issue (2): 36-38.doi: 10.19640/j.cnki.jtau.2018.02.009

富硒乳酸菌对肉鸡新城疫病毒抗体影响的研究

林耀玮, 刘燕霏, 杨建德^通信作者, 秦顺义

天津农学院动物科学与动物医学学院,天津 300384

收稿日期:2018-02-18 出版日期:2018-06-20 发布日期:2019-11-12
通讯作者: 杨建德（1969-）,男,教授,博士,研究方向为预防兽医学。E-mail：jiandeyang@126.com。
作者简介:林耀玮（1994-）,男,本科在读,研究方向为预防兽医学。E-mail：3111491142@qq.com。
基金资助:
国家自然科学基金项目（31101867）; 天津农学院附属动物医院项目（ZH004903）

Effect of se-enriched lactobacillus on antibody of NDV of broilers

LIN Yao-wei, LIU Yan-fei, YANG Jian-de^{Corresponding Author}, QIN Shun-yi

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-02-18 Online:2018-06-20 Published:2019-11-12

摘要/Abstract

摘要： 本研究旨在探究富硒乳酸菌对肉鸡新城疫病毒（NDV）抗体的影响。在静海县某鸡场抽选1日龄AA肉仔鸡1 000只,随机分2组,一组为基础日粮组,另一组为富硒乳酸菌组。单个试验组在组内随机分为5个重复试验组,每个重复组100只鸡。基础日粮组选用玉米豆粕型日粮作为饲料,富硒乳酸菌组日粮将2%富硒乳酸菌添加到玉米豆粕型日粮中。试验中雏鸡自由饮食和饮水,免疫方法参照常规免疫程序。试验期为42 d,分别在试验第14 d、28 d和42 d随机从每个重复组中选取2只肉鸡进行扑杀,采集血清,进行新城疫病毒抗体效价检测。结果表明,富硒乳酸菌能明显提高新城疫病毒血清抗体效价。

关键词: 富硒乳酸菌, 新城疫病毒, 血凝抑制试验, 抗体效价

Abstract: The purpose of this experiment is to study the effect of se-enriched lactobacillus on antibody of Newcastle Disease Virus (NDV) of broilers. One thousand day-old AA broiler chicken were selected from a chicken farm of Jinghai country, and the chicken were divided randomly into two groups which respectively had five repeat groups with 100 chickens each, one named basal diet group, and the other named se-enriched lactobacillus group. Corn soybean meal diet was selected for basal diet group, while 2% se-enriched lactobacillus was added into corn soybean meal diet for the other group. In this experiment, method of feeding was free diet, drinking and routine immunization program. This test period was 42 days. Two chicken were selected randomly in each repeat for slaughtering and collecting serums on 14^th, 28^th and 42^nd day. Assessments on the effect of se-enriched lactobacillus in antibody titers of NDV of broilers were made by hemagglutination-inhibition test. The result indicated that antibody titers of NDV was increased significantly by se-enriched lactobacillus.

Key words: se-enriched lactobacillus, NDV, hemagglutination-inhibition test, antibody titers

中图分类号:

S852.4

林耀玮, 刘燕霏, 杨建德, 秦顺义. 富硒乳酸菌对肉鸡新城疫病毒抗体影响的研究[J]. 天津农学院学报, 2018, 25(2): 36-38.

LIN Yao-wei, LIU Yan-fei, YANG Jian-de, QIN Shun-yi. Effect of se-enriched lactobacillus on antibody of NDV of broilers[J]. Journal of Tianjin Agricultural University, 2018, 25(2): 36-38.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2018.02.009

http://xuebao.tjau.edu.cn/CN/Y2018/V25/I2/36

参考文献

[1] 龚商羽. 一例肉种鸡硒缺乏引起的肌纤维变性[J]. 畜牧兽医科技信息,2017(5):102.
[2] 吴国杰,舒绪刚,尹国强,等. 硒饲料添加剂在动物养殖中的应用[J]. 广东饲料,2015,24(9):27-29.
[3] 史秋盛,吴娇. 硒对畜禽养殖的影响[J]. 中国畜牧兽医文摘,2014,30(9):198.
[4] 袁玲,许丽,徐基利,等. 乳酸菌菌体及全菌液制剂对雏鸡生长性能和盲肠菌群的影响[J]. 中国畜牧杂志,2011,47(13):59-62.
[5] 枉候军,黄兴国,谷雪玲. 乳酸菌的生物学功能及其在肉鸡生产中应用[J]. 湖南饲料,2015(3):25-27.
[6] 秦顺义,王宏祥,黄宝霞,等. 富硒乳酸菌对肉鸡生产性能和抗氧化功能的影响[J]. 黑龙江畜牧兽医,2015(3):22-24.
[7] 王宏祥,秦顺义,黄宝霞,等. 富硒乳酸菌对饲喂黄曲霉毒素日粮肉鸡生产性能和抗氧化功能的影响[J]. 畜牧与兽医,2014,46(6):87-89.
[8] 梁丛丛,胡治铭,国洪旭,等. 富硒益生菌对蛋鸡生产性能及鸡蛋中硒含量的影响[J]. 安徽农业科学,2015,43(5):99-100,210.
[9] Khorajiya J H,Pandey S,Ghodasara P D,et al.Patho-epidemiological study on Genotype-XIII Newcastle disease virus infection in commercial vaccinated layer farms[J]. Veterinary World,2015,8(3):372-381.
[10] 雷莉辉. 影响血凝试验和血凝抑制试验的因素分析[J].中国畜牧兽医,2013,40(6):244-246.
[11] 王丽娟. 鸡新城疫血凝和血凝抑制试验操作注意事项[J]. 畜牧兽医科技信息,2016(6):23-24.
[12] 蔡文杰,邱立新,邱美珍,等. 血凝和血凝抑制试验常见问题及解决方法[J]. 湖南畜牧兽医,2012(5):39-41.
[13] 曹萌,陈龙,许龙兵,等. 富硒乳酸菌对镉暴露机体自然杀伤细胞活性调节[J]. 中国公共卫生,2005,21(6):681-682.
[14] 杨家军. 富硒益生菌制剂工业化生产条件及其抑制致病性大肠杆菌的研究[D]. 南京:南京农业大学,2008.
[15] Chuang L,Wu K G,Pai C,et al.Heat-killed cells of lactobacilli skew the immune response toward T helper 1 polarization in mouse splenocytes and dendritic cell- treated T cells[J]. J Agric Food Chem,2007,55(26):11080-11086.

富硒乳酸菌对肉鸡新城疫病毒抗体影响的研究

Effect of se-enriched lactobacillus on antibody of NDV of broilers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	傅春妮, 周磊, 王欢欢, 李元辉, 秦顺义. 富硒乳酸菌对小鼠生长、血液生化指标、肠道菌群和细胞因子的影响[J]. 天津农学院学报, 2020, 27(3): 49-52.
[2]	张曼, 柳溪, 丛日华. 卵黄抗体提取方法的优化[J]. 天津农学院学报, 2020, 27(2): 56-60.
[3]	田澍瑶, 杨雨, 胡玥, 武志伟, 张子卉, 袁艳阳, 李富桂. 15种免疫增效剂对鸡新城疫免疫效果影响的对比研究[J]. 天津农学院学报, 2020, 27(1): 50-52.
[4]	高静, 董东晓, 龙雨清, 李萌, 李留安, 金天明. 新城疫病毒HN蛋白的抑瘤机制及其研究进展[J]. 天津农学院学报, 2014, 21(3): 49-52.