5-aza-dC联合TSA对不同供体细胞来源牛核移植胚胎体外发育的影响

天津农学院学报 ›› 2012, Vol. 19 ›› Issue (2): 19-23.

5-aza-dC联合TSA对不同供体细胞来源牛核移植胚胎体外发育的影响

丁向彬, 王轶敏, 葛秀国, 刘新峰, 李吉霞, 郭宏^通讯作者

天津农学院动物科学系,天津 300384

收稿日期:2012-05-03 出版日期:2012-06-20 发布日期:2019-11-12
通讯作者: 郭宏（1964-）,男,内蒙古通辽人,教授,博士,研究方向为动物分子育种。E-mail：guohong882003@yahoo.com.cn。
作者简介:丁向彬（1978-）,男,河南南阳人,讲师,博士,研究方向为动物胚胎工程与繁殖生物技术。联系电话：（022）23781297。
基金资助:
转基因生物新品种培育重大专项“高产转基因肉牛新品种培育”（2008zx08007-002）; 天津农学院科学研究发展基金计划项目“高效牛体细胞核移植技术体系建立的研究”（2009D06）

Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells

DING Xiang-bin, WANG Yi-min, GE Xiu-guo, LIU Xin-feng, LI Ji-xia, GUO Hong^{Corresponding Author}

Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China

Received:2012-05-03 Online:2012-06-20 Published:2019-11-12

摘要/Abstract

摘要： 为研究5-aza-dC和TSA提高核移植胚胎体外发育能力的作用是否有供体细胞特异性,实验选择皮肤成纤维细胞、胎儿成纤维细胞和卵丘颗粒细胞作为核供体细胞进行核移植,联合使用20 nM（72 h） 5-aza-dC和50 nM（12 h） TSA处理供体细胞和重构胚,比较5-aza-dC+TSA对不同供体细胞来源核移植胚胎体外发育的影响。实验结果表明,经5-aza-dC+TSA处理后,极显著提高了3种供体细胞来源核移植胚胎的体外囊胚发育率（P<0.01）。虽然胎儿成纤维细胞和卵丘颗粒细胞得到的发育率稍高于皮肤成纤维细胞组,但各对照组之间以及各处理组之间的体外发育率没有明显差异,5-aza-dC和TSA对核移植胚胎体外发育的影响没有供体细胞特异性。

关键词: 牛, 5-aza-dC, TSA, 核移植, 供体细胞

Abstract: ：To investigate the effects of 5-aza-dC and TSA on the development of embryos cloned from different donor cells, the fetal fibroblast cells, adult skin fibroblast cells, and cumulus cells were used as donor cells for nuclear transfer, the donor cells and cloned embryos were treated with TSA（50 nM, 12 h） and 5-aza-dC（20 nM, 72 h）, and the effects of these reagents on the development of cloned embryos were investigated. The results show that 5-aza-dC combined with TSA could significantly promoted the development potential of cloned embryos （P<0.01）, and the beneficial effects of TSA and 5-aza-dC on cloned embryos development were not of specific characteristics for the donor cells.

Key words: bovine, 5-aza-dC, TSA, nuclear transfer, donor cell

中图分类号:

S814.6

丁向彬, 王轶敏, 葛秀国, 刘新峰, 李吉霞, 郭宏. 5-aza-dC联合TSA对不同供体细胞来源牛核移植胚胎体外发育的影响[J]. 天津农学院学报, 2012, 19(2): 19-23.

DING Xiang-bin, WANG Yi-min, GE Xiu-guo, LIU Xin-feng, LI Ji-xia, GUO Hong. Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells[J]. Journal of Tianjin Agricultural University, 2012, 19(2): 19-23.

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链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2012/V19/I2/19

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