牛骨骼肌卫星细胞中miR-143互作lncRNA-HZ5的鉴定及其相互调控作用分析

doi:10.19640/j.cnki.jtau.2019.03.012

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (3): 51-56.doi: 10.19640/j.cnki.jtau.2019.03.012

牛骨骼肌卫星细胞中miR-143互作lncRNA-HZ5的鉴定及其相互调控作用分析

王轶敏, 张蔚然, 张俊星, 刘新峰, 郭宏, 丁向彬^通信作者

天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300384

收稿日期:2019-06-18 出版日期:2019-09-30 发布日期:2019-09-30
通讯作者: 丁向彬（1978–）,男,教授,博士,研究方向为动物细胞工程、动物繁育生物技术。E-mail：xiangbinding@163.com。
作者简介:王轶敏（1980–）,女,讲师,博士,研究方向为动物细胞与转基因工程。E-mail：wangyimin112@aliyun.com。张蔚然（1992–）,男,硕士在读,研究方向为动物细胞与转基因工程。E-mail：zhangweiran1992@sina.com。王轶敏和张蔚然并列为第一作者。
基金资助:
国家自然科学基金资助项目（31201021）; 天津市“131”创新型人才培养工程第一层次人选计划项目（无编号）; 天津市高校“中青年骨干创新人才培养计划”人选项目（无编号）

The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells

WANG Yi-min, ZHANG Wei-ran, ZHANG Jun-xing, LIU Xin-feng, GUO Hong, DING Xiang-bin

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-06-18 Online:2019-09-30 Published:2019-09-30

摘要/Abstract

摘要： 为探究miR-143与其互作lncRNA之间的关系及其在牛骨骼肌卫星细胞成肌分化过程中的潜在作用机制,选取两个表达丰度较高miR-143的预测互作lncRNA（lncRNA-HZ5和lncRNA-HZ8）进行研究。采用双荧光素酶试验验证miR-143对预测互作lncRNA的靶向调控作用,采用qRT-PCR技术检测miR-143表达水平改变对预测互作lncRNA表达的影响,并采用qRT-PCR和western blot技术检测干扰互作lncRNA表达后对miR-143表达水平及miR-143靶基因表达水平的影响。双荧光素酶试验结果表明,miR-143可直接调控lncRNA-HZ5的表达;在牛肌卫星细胞中过表达miR-143后,lncRNA-HZ5的表达水平显著下调,而抑制miR-143表达后,lncRNA-HZ5的表达水平显著上调;采用siRNA干扰lncRNA-HZ5的表达,发现抑制lncRNA-HZ5表达能够下调miR-143表达,并上调miR-143靶基因IGFBP5的蛋白水平。研究结果表明,在牛骨骼肌卫星细胞中miR-143可以和lncRNA-HZ5发生相互调控作用,其对牛肌卫星细胞成肌分化的调控作用可能是通过互作来完成的。

关键词: miR-143, lncRNA-HZ5, 牛, 骨骼肌卫星细胞, 成肌分化

Abstract: In order to explore the relationship between miR-143 and its interacted lncRNA and its mechanism in the process of bovine myogenic differentiation, two predicted highly expressed（lncRNA lncRNA-HZ5 and lncRNA-HZ8）were chosen for further research. The relationship between miR-143 and predicted interacted lncRNA were identified by dual-luciferase assay and qRT-PCR detection, the influence of lncRNA on the protein level of miR-143’s target gene was detected by western blot. The results showed that lncRNA-HZ5 was directly regulated by miR-143 using dual-luciferase assay. The expression of lncRNA-HZ5 was significantly down-regulated when miR-143 was over expressed, and significantly up-regulated when miR-143 was inhibited. It was found that inhibition of lncRNA-HZ5 expression using siRNA could down-regulate miR-143 and up-regulate the protein level of miR-143’s target gene IGFBP5. The results showed that the miR-143 could interact with lncRNA-HZ5 in bovine skeletal muscle satellite cells, which indicated that miR-143 may regulate the bovine myogenic differentiation by interacting with lncRNA-HZ5.

Key words: miR-143, lncRNA-HZ5, bovine, skeletal muscle satellite cells, myogenic differentiation

中图分类号:

S814.8

王轶敏, 张蔚然, 张俊星, 刘新峰, 郭宏, 丁向彬. 牛骨骼肌卫星细胞中miR-143互作lncRNA-HZ5的鉴定及其相互调控作用分析[J]. 天津农学院学报, 2019, 26(3): 51-56.

WANG Yi-min, ZHANG Wei-ran, ZHANG Jun-xing, LIU Xin-feng, GUO Hong, DING Xiang-bin. The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 51-56.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2019.03.012

http://xuebao.tjau.edu.cn/CN/Y2019/V26/I3/51

参考文献

[1] Amaral P P,Mattick J S.Noncoding RNA in development[J]. Mamm Genome,2008,19(7-8):454–492.
[2] Xing C,Gui Y Y.Novel human lncRNA-disease association inference based on lncRNA expression profiles[J]. Bioinformatics,2013,29(20):2617–2624.
[3] Kretz M,Siprashvili Z,Chu C,et al.Control of somatic tissue differentiation by the long non-coding RNA TINCR[J]. Nature,2013,493(7431):231–235.
[4] Van R E,Liu N,Olson E N.MicroRNAs flex their muscles[J]. Trends Genet,2008,24(24):159–166.
[5] Watanabe T,Sato T,Amano T,et al.Dnm3os,a non-coding RNA,is required for normal growth and skeletal development in mice[J]. Developmental Dynamics An Official Publication of the American Association of Anatomists,2008,237(12):3738–3748.
[6] Caretti G,Schiltz R L,Dilworth F J,et al.The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation[J]. Developmental Cell,2006,11(4):547–560.
[7] Cesana M,Cacchiarelli D,Legnini I,et al.A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA[J]. Cell,2011,147(2):358–369.
[8] Zhang W R,Zhang H N,Wang Y M,et al.MiR-143 regulates proliferation and differentiation of bovineskeletal muscle satellite cells by targeting IGFBP5[J]. In Vitro Cellular & Developmental Biology-Animal,2017,53(3):265–271.
[9] 王轶敏,代阳,刘新峰,等. 牛骨骼肌卫星细胞的分离鉴定和诱导分化[J]. 中国畜牧兽医,2014,41(7):142-147.
[10] Dai Y,Wang Y M,Zhang W R,et al.The role of micro RNA-1 and microRNA-206 in the proliferation and differentiation of bovine skeletal muscle satellite cells[J]. In Vitro Cellular & Developmental Biology-Animal,2016,52(1):27–34.
[11] 丁向彬,张蔚然,张俊星,等. lncRNA-HZ5对牛骨骼肌卫星细胞成肌分化的调控作用研究[J]. 天津农学院学报,2017,24(3):64–68.
[12] Kallen A N,Zhou X B,Xu J,et al.The imprinted H19 lncRNA antagonizes let-7 microRNAs[J]. Mol Cell,2013,52:101–112.
[13] Yoon J H,Abdelmohsen K,Srikantan S,et al.LincRNA- p21 suppresses target mRNA translation[J]. Mol Cell,2012,47:648–655.
[14] Yoon J H,Abdelmohsen K,Kim J,et al.Scaffold function of long noncoding RNA HOTAIR in protein ubiquitination[J]. Nat Commun,2013,4:2939.
[15] Liu Q,Huang J,Zhou N,et al.lncRNA loc285194 is a p53-regulated tumor suppressor[J]. Nucleic Acids Res,2013,41:4976–4987.
[16] Keniry A,Oxley D,Monnier P,et al.The H19 lincRNA is developmental reservoir of miR-675 that suppresses growth and IGF1R[J]. Nature Cell Biology,2012,7(14):659–665.
[17] Poliseno L,Salmena L,Zhang J,et al.A coding- independent function of gene and pseudogene mRNAs regulates tumourbiology[J]. Nature,2010,465(7301):1033.
[18] Faghihi M A,Zhang M,Huang J,et al.Evidence for natural antisense transcript-mediated inhibition of microRNA function[J]. Genome Biol,2010,11(5):56.

牛骨骼肌卫星细胞中miR-143互作lncRNA-HZ5的鉴定及其相互调控作用分析

The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李彦, 梁丽雅, 乔芳, 谢秋建, 马俪珍. 改进工艺条件对红烧卤牛肉出品率和感官品质的影响[J]. 天津农学院学报, 2023, 30(5): 48-53.
[2]	胡蕾, 王轶敏, 张林林, 李新, 郭益文, 丁向彬. UCH-L3通过影响RAD51调控牛骨骼肌卫星细胞增殖与成肌分化[J]. 天津农学院学报, 2023, 30(2): 1-6.
[3]	张龙豪, 张建斌, 郭鹏, 史云潇, 安雪东. 奶牛精准饲喂决策系统设计[J]. 天津农学院学报, 2021, 28(1): 53-58.
[4]	王宏璐, 黄文静, 黄志伟, 原尚奇, 解诗雨, 吴楠. 二维相关中红外光谱判别牛奶中的四环素[J]. 天津农学院学报, 2020, 27(3): 79-82.
[5]	王鹏, 赵润, 孟祥辉, 付学周, 辛悦, 宁天阳, 赵文雅, 杨仁杰. 近红外漫反射光谱结合CARS-PLS规模化奶牛场粪便总氮定量分析模型的建立[J]. 天津农学院学报, 2020, 27(1): 82-86.
[6]	樊晓盼, 杨谨喻, 施煜, 刘一鸣, 韩芸, 马俪珍. 牛肉调味基料在贮藏过程中的品质变化[J]. 天津农学院学报, 2019, 26(1): 54-58.
[7]	徐琛善, 吴楠, 海霞, 王军, 田佳, 刘香檬, 鲍振博, 彭锦星. 中红外光谱快速检测牛奶中土霉素的可行性研究[J]. 天津农学院学报, 2018, 25(3): 70-73.
[8]	温海蔚, 刘燕霏, 杨建德. 青海省互助县牛衣原体病的血清学调查[J]. 天津农学院学报, 2017, 24(4): 46-47.
[9]	张海洋, 廖彩淇, 杨仁杰, 鲍秀君, 王威, 靳皓, 张伟玉. 基于近、中红外光谱数据融合掺假牛奶的判定[J]. 天津农学院学报, 2017, 24(4): 52-56.
[10]	刘燕霏, 杨建德. 奶牛乳腺免疫学研究进展[J]. 天津农学院学报, 2017, 24(4): 81-84.
[11]	丁向彬, 张蔚然, 张俊星, 王轶敏, 刘新峰, 郭宏. lncRNA-HZ5对牛骨骼肌卫星细胞成肌分化的调控作用研究[J]. 天津农学院学报, 2017, 24(3): 64-68.
[12]	樊晓盼, 仇泓博, 马俪珍. 响应面法优化牛骨肉蛋白液酶解工艺条件[J]. 天津农学院学报, 2017, 24(3): 73-78.
[13]	樊晓盼, 张伯男, 张甜, 马俪珍. 响应面法优化热压浸提牛骨蛋白提取工艺[J]. 天津农学院学报, 2017, 24(2): 58-62.
[14]	张钧, 王月, 黄培操, 管贤贤, 卢赫宇, 孙洋洋, 石利军. 牛粪水稻秸秆厌氧共发酵产沼气操作参数优化[J]. 天津农学院学报, 2017, 24(1): 58-61.
[15]	刘称,王英超, 金红, 马晓雨, 史雪艳. 牛蒡子中牛蒡苷的超声提取工艺优化研究[J]. 天津农学院学报, 2016, 23(4): 28-32.