恩诺沙星对眼斑双锯鱼生理生化的影响

doi:10.19640/j.cnki.jtau.2019.03.014

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (3): 62-67.doi: 10.19640/j.cnki.jtau.2019.03.014

恩诺沙星对眼斑双锯鱼生理生化的影响

高鸿伟^1,2,3, 胡静^2,3,4, 杨蕊^2,3,4, 周胜杰^2,3,4, 于刚^2,3,4, 马振华^{2,3,4,通信作者}

1. 仲恺农业工程学院,广州 510225;
2. 中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚 572018;
3. 农业农村部南海渔业资源开发利用重点实验室,广州 510300;
4. 三亚热带水产研究所,海南三亚 572018

收稿日期:2019-03-16 出版日期:2019-09-30 发布日期:2019-09-30
通讯作者: 马振华（1981-）,男,副研究员,博士,主要从事海水鱼类繁育研究。E-mail：zhenhua.ma@hotmail.com。
作者简介:高鸿伟（1996-）,男,本科在读,主要从事鱼类苗种繁育研究。E-mail：906340833@qq.com。
基金资助:
三亚市专项科研试制项目（2018KS16）

Effects of enrofloxacin on the physiology and biochemistry of Amphiprion ocellaris

GAO Hong-wei^1,2,3, HU Jing^2,3,4, YANG Rui^2,3,4, ZHOU Sheng-jie^2,3,4, YU Gang^2,3,4, MA Zhen-hua^2,3,4

1. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Tropical Aquaculture Research and Development Centre, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, Hainan Province, China;
3. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou 510300, China;
4. Sanya Tropical Fisheries Research Institute, Sanya 572018, Hainan Province, China

Received:2019-03-16 Online:2019-09-30 Published:2019-09-30

摘要/Abstract

摘要： 为了揭示恩诺沙星对眼斑双锯鱼生理生化的影响,采用3个不同浓度（0、5、10 mg/L）的恩诺沙星浸泡眼斑双锯鱼幼鱼,对其消化酶、免疫酶活性和氧化产物含量测定分析。结果表明,在消化酶中,恩诺沙星对眼斑双锯鱼幼鱼胰蛋白酶和胃蛋白酶活性影响最为显著,脂肪酶次之,淀粉酶影响较小;恩诺沙星对肝脏免疫酶活性的影响程度顺序为谷胱甘肽过氧化物酶（GSH）>碱性磷酸酶（AKP）、溶菌酶（LZM）、谷胱甘肽转移酶（GST）>超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）;恩诺沙星对氧化产物含量的影响程度为LPO（过氧化脂质）较丙二醛（MDA）显著。恩诺沙星对眼斑双锯鱼的各类酶活性及氧化产物含量存在影响,且浸泡处理影响存在显著性差异（P<0.05）。该结果对眼斑双锯鱼的疾病治疗具有重要意义。

关键词: 眼斑双锯鱼, 恩诺沙星, 免疫酶, 消化酶, 氧化产物

Abstract: To study the effects of different concentrations of enrofloxacin treatments on physiology and biochemistry of A. ocellaris, enrofloxacin soaking experiments（0, 5, 10 mg/L）were performed on three groups of A. ocellaris. The results showed that among the digestive enzymes activities, enrofloxacin had the most significant effect on trypsin and pepsin, followed by lipase and amylase. The order of enrofloxacin on the liver immune enzymes of the individuals was glutathione peroxidase（GSH）>alkaline phosphatase（AKP）, lysozyme（LZM）, glutathione S-transferase（GST）>superoxide dismutase（SOD）, catalase（CAT）, peroxidase（POD）. The influence of oxidation products is more significant in lipid peroxide（LPO ）than malondialdehyde（MDA）. Enrofloxacin had an effect on the activities of various enzymes and the content of oxidized products in A. ocellaris, and the effect of soaking treatment had significant difference（P<0.05）. This result is of great significance for the disease treatment of the A. ocellaris.

Key words: Amphiprion ocellaris, enrofloxacin, immune enzymes, digestive enzymes, oxidation products

中图分类号:

S965.8

高鸿伟, 胡静, 杨蕊, 周胜杰, 于刚, 马振华. 恩诺沙星对眼斑双锯鱼生理生化的影响[J]. 天津农学院学报, 2019, 26(3): 62-67.

GAO Hong-wei, HU Jing, YANG Rui, ZHOU Sheng-jie, YU Gang, MA Zhen-hua. Effects of enrofloxacin on the physiology and biochemistry of Amphiprion ocellaris[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 62-67.

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恩诺沙星对眼斑双锯鱼生理生化的影响

Effects of enrofloxacin on the physiology and biochemistry of Amphiprion ocellaris

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