响应面法优化雨生红球藻产虾青素培养条件

doi:10.19640/j.cnki.jtau.2019.03.013

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (3): 57-61.doi: 10.19640/j.cnki.jtau.2019.03.013

响应面法优化雨生红球藻产虾青素培养条件

李嘉仪, 窦勇, 邵蓬, 高金伟, 贾旭颖, 张文慧, 王祎哲, 马婷, 周文礼^通信作者

天津农学院天津市渔业资源与环境研究室,天津 300384

收稿日期:2019-02-27 出版日期:2019-09-30 发布日期:2019-09-30
通讯作者: 周文礼（1969-）,男,研究员,博士,研究方向为生物铒料培养与应用。E-mail：wenlizhou@tjau.edu.cn。
作者简介:李嘉仪（1994-）,女,硕士在读,研究方向为渔业资源保护与环境修复。E-mail：476984236@qq.com。
基金资助:
天津市水产产业技术体系创新团队项目（ITTFRS2017005）; 天津市科技重大专项与工程项目（15ZXBFNC00120）; 卫星海洋环境动力学国家重点实验室开放基金项目（SOED1419）; 农业部南海渔业资源开发利用重点实验室开放基金项目（FREU2015-04）; 天津市水产生态及养殖重点实验室开放基金项目（TJAE201805）

Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology

LI Jia-yi, DOU Yong, SHAO Peng, GAO Jin-wei, JIA Xu-ying, ZHANG Wen-hui, WANG Yi-zhe, MA Ting, ZHOU Wen-li

Tianjin Fisheries Resources and Environment Research Office, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-02-27 Online:2019-09-30 Published:2019-09-30

摘要/Abstract

摘要： 雨生红球藻是一种重要的资源微藻,产物虾青素具有超强抗氧化活性,因而广泛被应用于医药和食品等领域。为雨生红球藻实现高产虾青素,研究了3种胁迫条件,光照强度（5 000、7 000、9 000 lx）、硝酸钠浓度（0、0.13、0.25 g/L）和氯化钠浓度（0、2.5、5.0 g/L）对雨生红球藻的虾青素含量的影响,利用响应面法对胁迫条件进行优化。3因素对产生虾青素含量的影响顺序为：氯化钠浓度>光照强度>硝酸钠浓度。优化得到的最佳提取条件为光照强度9 000 lx、硝酸钠浓度0 g/L、氯化钠浓度2.5 g/L,在此条件下虾青素含量可达6.8 mg/L,与预测值相近,表明模型具有较高可靠性。

关键词: 雨生红球藻, 虾青素, 光照强度, 响应面

Abstract: Haematococcus pluvialis is an important resource microalgae. The product astaxanthin has excellent antioxidant activity and is widely used in medicine and food. In order to study the high-yield astaxanthin of Haematococcus pluvialis, three stress conditions（5 000, 7 000, 9 000 lx）, sodium nitrate concentrations（0, 0.13, 0.25 g/L）and chlorination were studied. The effects of sodium concentrations（0, 2.5, 5.0 g/L）on astaxanthin content of Haematococcus pluvialis were optimized by response surface methodology. The order of influence of the three factors on the production of astaxanthin content was: sodium chloride concentration>light intensity>sodium nitrate concentration. The optimal extraction conditions were: light intensity 9 000 lx, sodium nitrate concentration 0 g/L, sodium chloride concentration 2.5 g/L, under which the astaxanthin content could reach 6.8 mg/L, and it was similar to the predicted value. The results indicated that the model has high reliability.

Key words: Haematococcus pluvialis, astaxanthin, light intensity, response surface

中图分类号:

DF413.4

李嘉仪, 窦勇, 邵蓬, 高金伟, 贾旭颖, 张文慧, 王祎哲, 马婷, 周文礼. 响应面法优化雨生红球藻产虾青素培养条件[J]. 天津农学院学报, 2019, 26(3): 57-61.

LI Jia-yi, DOU Yong, SHAO Peng, GAO Jin-wei, JIA Xu-ying, ZHANG Wen-hui, WANG Yi-zhe, MA Ting, ZHOU Wen-li. Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 57-61.

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响应面法优化雨生红球藻产虾青素培养条件

Optimization of culture conditions for astaxanthin production by Haematococcus pluvialis with response surface methodology

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