天津农学院学报 ›› 2025, Vol. 32 ›› Issue (5): 18-23.doi: 10.19640/j.cnki.jtau.2025.05.004

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水杨酸和壳聚糖对盐胁迫下毛叶苕子幼苗生理特性的影响

王丹a, 田秀平b,通信作者, 杜锦b   

  1. 天津农学院 a.园艺园林学院; b.农学与资源环境学院,天津 300392
  • 收稿日期:2024-09-05 发布日期:2025-11-12
  • 通讯作者: 田秀平(1965—),女,教授,博士,从事植物营养和生理研究。E-mail:tian5418@sina.com。
  • 作者简介:王丹(1979—),女,副教授,硕士,从事植物生理及解剖结构研究。E-mail:16590358@qq.com。
  • 基金资助:
    国家绿肥产业技术体系项目(CARS-22); 天津市农业科技成果转化项目(202101090)

Effect of salicylic acid and chitosan on seedling physiological characters of Vicia villosa Roth under salt stress

Wang Dana, Tian Xiupingb,Corresponding Author, Du Jinb   

  1. a. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China; b. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2024-09-05 Published:2025-11-12

摘要: 针对土壤盐渍化严重的问题,以‘天毛苕2'毛叶苕子为材料,研究外源水杨酸和壳聚糖对盐胁迫下毛叶苕子幼苗生理特性的影响。在土壤含盐量不同的条件下,分别用1 mmol/L水杨酸和0.30%壳聚糖处理毛叶苕子幼苗,测定叶片中过氧化氢酶、超氧化物歧化酶、可溶性糖、可溶性蛋白、脯氨酸和丙二醛含量。结果表明:在土壤含盐量为0.38%时,幼苗中过氧化氢酶活性、超氧化物歧化酶活性、可溶性糖含量、可溶性蛋白含量和脯氨酸的含量分别比对照提高了8.37%和9.43%、7.45%和8.41%、4.21%和11.73%、32.01 %和38.50 %、9.52%和11.90%(P<0.05),丙二醛含量分别降低了15.06%和17.85%(P<0.05);在土壤含盐量为0.70%时,壳聚糖处理毛叶苕子幼苗后可溶性糖和可溶性蛋白含量分别比对照提高了8.25%和13.90%(P<0.05);水杨酸处理后可溶性糖和可溶性蛋白含量与对照间差异不显著。试验表明,水杨酸和壳聚糖可有效缓解盐胁迫对毛叶苕子幼苗的影响,且0.30%壳聚糖的作用优于1mmol/L 水杨酸。

关键词: 毛叶苕子, 抗氧化酶, 渗透调节物, 盐胁迫, 外源物

Abstract: To solve the problem of increasing soil salinization, the effects of exogenous salicylic acid and chitosan on the physiological characteristics of Vicia villosa Roth seedlings were studied. ‘Tianshi 2' V. villosa Roth as the material, with 1 mmol/L salicylic acid and 0.30% chitosan treated, under different salt-containing soil conditions, catalase activity, superoxide dismutase activity, soluble sugar content, soluble protein content, proline content and malondialdehyde content. Were deter mined. Conpared with the contrd greup, the cidalase activity, super c side disrn utase activity, soluble sugur conreit, soluble prolein content and proune content of 1 mmol/L solicguc acid and 0.30% chitosan treated V. Viuosa Roth seedling increased 8.37% and 9.43%, 7.45% and 8.41%, 4.21% and 11.73%, 32.01% and 38.50%, 9.52% and 11.90%(P<0.05)respeetively under 0.38% salt centent. The content of malondialdehyde was decreased by 15.06% and 17.85%(P<0.05), respectively. The content of soluble sugar and soluble protein of V. villosa Roth seedling treated with 0.30% chitosan were higher 8.25% and 13.90%(P<0.05)than control respectively under 0.70% salt content. There was no significant difference in the contents of the two substances between the 1 mmol/L salicylic acid treatment and control of V. villosa Roth seedlings under 0.70% salt content. Salicylic acid and chitosan can effectively alleviate the impact of salt stress on the seedlings of V. villosa Roth seeds, and the degree of 0.30% chitosan is better than 1 mmol/L salicylic acid.

Key words: Vicia villosa Roth, antioxidant enzymes, osmotic regulator, salt stress, exogenous substances

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