天津农学院学报 ›› 2023, Vol. 30 ›› Issue (3): 16-20.doi: 10.19640/j.cnki.jtau.2023.03.004

• 研究与简报 • 上一篇    下一篇

以芦苇为碳源进行灵芝液体菌种发酵条件的优化

刘静雯, 辛世纪, 王竟夷, 连洁, 黄亮通信作者, 班立桐   

  1. 天津农学院 农学与资源环境学院,天津 300392
  • 收稿日期:2021-11-18 出版日期:2023-06-30 发布日期:2023-09-06
  • 通讯作者: 黄亮(1983—),男,副教授,博士,主要从事食用菌技术研究。E-mail:huangliang@tjau.edu.cn。
  • 作者简介:刘静雯(1995—),女,硕士在读,主要从事菌类作物栽培方面研究。E-mail:995426796@qq.com。
  • 基金资助:
    天津市蔬菜产业技术体系创新团队项目(ITTVRS2021016); 中央引导地方科技发展专项(20ZYCGSN00330); 天津市“131”创新型人才培养工程项目(无编号)

Optimization of fermentation conditions of Ganoderma lucidum liquid strain using reed as carbon source

Liu Jingwen, Xin Shiji, Wang Jingyi, Lian Jie, Huang LiangCorresponding Author, Ban Litong   

  1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2021-11-18 Online:2023-06-30 Published:2023-09-06

摘要: 以芦苇秸秆为培养基碳源,以灵芝菌丝体产量为指标,采用单因素试验和正交试验确定了最佳培养基配方及培养条件。结果表明:培养基配方为芦苇秸秆0.5%、酵母浸粉1.5%、KH2PO4 0.15%、MgSO4 0.075%、pH为6,培养条件为转速150 r/min、温度25 ℃时,灵芝菌丝体产量最高,菌丝干重为12.20 g/L,较优化前提高了1.51倍。

关键词: 芦苇秸秆, 灵芝, 液体发酵, 正交试验

Abstract: With reed stalks as the carbon source of the medium and the yield of Ganoderma lucidum mycelium as an indicator, the optimal medium formula and culture conditions were determined by single factor experiment and orthogonal experiment. The results showed that the medium formula was 0.5% of reed straw, 1.5% of yeast extract, 0.15% of KH2PO4, 0.075% of MgSO4, pH of 6, and the culture condition was 150 r/min at a temperature of 25 ℃. Under the condition, the yield of Ganoderma lucidum mycelium was the highest. The dry weight of mycelium was 12.20 g/L, which was 1.51 times higher than before optimization.

Key words: reed straw, Ganoderma lucidum, liquid fermentation, orthogonal test

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