组学方法在植物保卫细胞研究中的应用

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (4): 71-76.

组学方法在植物保卫细胞研究中的应用

吴天文^1a, 王俊斌^1b, 李明^1a, 陈小强^1a, 曹高燚^1a, 谢晓东^{1a,通信作者}

1. 天津农学院 a. 农学与资源环境学院,b. 基础科学学院,天津 300384

收稿日期:2017-05-15 出版日期:2017-12-31 发布日期:2019-10-15
通讯作者: 谢晓东（1975-）,男,内蒙古突泉人,研究员,博士,主要从事小麦抗逆分子基础研究。E-mail: xiex@tjau.edu.cn。
作者简介:吴天文（1990-）,男,安徽安庆人,硕士在读,主要从事小麦抗逆分子基础研究。E-mail:1053573415@qq.com。
基金资助:
国家自然科学基金项目“小麦保卫细胞中ABA信号通路新组分的发掘及功能研究”（31671611）; 天津市应用基础与前沿技术研究计划一般项目“小麦TaWRKY71a调控植物响应热胁迫的机理研究”（14JCYBJC30600）; 天津市“131”创新型人才团队“作物抗逆的机理及分子育种创新团队”（2015-20）

Application of Omics Methods in Studies of Plant Guard Cells

WU Tian-wen^1a, WANG Jun-bin^1b, LI Ming^1a, CHEN Xiao-qiang^1a, CAO Gao-yi^1a, XIE Xiao-dong^{1a,Corresponding Author}

1. Tianjin Agricultural University, a. College of Agronomy and Resource Environment, b. College of Basic Science, Tianjin 300384, China

Received:2017-05-15 Online:2017-12-31 Published:2019-10-15

摘要/Abstract

摘要： 保卫细胞是植物表皮的特化细胞,通过控制气孔运动,来调节植物体与环境水分和气体的交换,在植物应答逆境胁迫中发挥重要作用。与其他表皮细胞相比,保卫细胞具有功能特异性,因此,解析其生物学功能,有必要分离出单一细胞类型的保卫细胞。此外,深入解析保卫细胞的功能机制,还需应用近年来快速发展的组学方法。本文主要综述了近年来双子叶植物保卫细胞分离技术,以及以保卫细胞为材料进行转录组、蛋白组及代谢组分析方面的研究进展,对单子叶植物的相关研究具有很好的借鉴意义。

关键词: 保卫细胞, 分离技术, 组学研究

Abstract: Guard cells as specialized epidermal cells, play important roles in plant responses to environmental stresses through controlling stomatal movement, thereby plant water loss and gas exchange. Compared with other epidermal cells, guard cells demonstrate functional specificity. Therefore, isolation of single-type guard cells is necessary for clarifying the biological functions of guard cells with the application of fast developed omics methods. In this review, we summarized the recent advances in guard cells protoplast isolation of dicotyledonous plants and omics studies including transcriptomic, proteomic and metablomic data analysis, and provided well established references for the relevant studies in monocotyledonous crops.

Key words: guard cells, separation technology, omics studies

中图分类号:

Q756

吴天文, 王俊斌, 李明, 陈小强, 曹高燚, 谢晓东. 组学方法在植物保卫细胞研究中的应用[J]. 天津农学院学报, 2017, 24(4): 71-76.

WU Tian-wen, WANG Jun-bin, LI Ming, CHEN Xiao-qiang, CAO Gao-yi, XIE Xiao-dong. Application of Omics Methods in Studies of Plant Guard Cells[J]. Journal of Tianjin Agricultural University, 2017, 24(4): 71-76.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2017/V24/I4/71

参考文献

[1] Hetherington A M.Guard cell signaling[J]. Cell,2001,107(6):711-714.
[2] Kim T H,Bohmer M,Hu H,et al.Guard cell signal transduction network: Advances in understanding abscisic acid,CO₂,and Ca²+ signaling[J]. Annual Review of Plant Biology,2010,61(1):561-591.
[3] Acharya B R,Assmann S M.Hormone interactions in stomatal function[J]. Plant Molecular Biology,2009,69(4):451-462.
[4] Wang J,Guo Y,Ding B,et al.Screening of stomatal mutants in Arabidopsis,using a novel controlled environmental infrared imaging system[J]. Plant Growth Regulation,2016,79(2):157-165.
[5] 魏琳,张卫国,任柏林,等. 小麦离体表皮上气孔对若干关键生理因子的应答反应[J]. 植物生理学报,2015,51(5):649-654.
[6] 陈德龙,叶映微,刘丽红,等. 植物保卫细胞的激素信号转导网络研究进展[J]. 核农学报,2016,30(1):65-71.
[7] Bates G W,Rosenthal D M,Sun J,et al.A Comparative study of the Arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose[J]. Plos One,2012,7(11):e49641.
[8] Pandey S,Wang X S,Assmann S M.Preparation and applications of Arabidopsis thaliana guard cell protoplasts[J]. New Phytologist,2002,153(3):517-526.
[9] Adrian J,Chang J,Ballenger C E,et al.Transcriptome dynamics of the stomatal lineage: Birth,amplification,and termination of a self-renewing population[J]. Developmental Cell,2015,33(1):107-118.
[10] 陈怡. 高通量测序技术及其发展[J]. 科学中国人,2016(17):25.
[11] 陈焕文,胡斌,张燮. 复杂样品质谱分析技术的原理与应用[J]. 分析化学,2010,38(8):1069-1088.
[12] Aubry S,Aresheva O,Reyna-Liorens I,et al.A specific transcriptome signature for guard cells from the C₄ plant Gynandropsis gynandra[J]. Plant Physiology,2016,170(3):1345-1357.
[13] Zeiger E,Helper P K.Production of guard cell protoplasts from onion and tobacco[J]. Plant Physiology,1976,58(4):492-498.
[14] Nisita O,Shweta P,Maeli M.Guard cell purification and RNA isolation suitable for high-throughput transcriptional analysis of cell-type responses to biotic stresses[J]. Molecular Plant-Microbe Interactions,2013,26(8):844-849.
[15] Fitzsimons P J,Weyers J D B. Separation and purification of protoplast types from Commelina communis L. leaf epidermis[J]. Journal of Experimental Botany,1983,34(1):55-66.
[16] Kruse T,Tallman G,Zeiger E.Isolation of guard cell protoplasts from mechanically prepared epidermis of Vicia faba leaves[J]. Plant Physiology,1989,90(4):1382-1386.
[17] Zhu M,Jeon B W,Geng S,et al.Preparation of epidermal peels and guard cell protoplasts for cellular,electrophy- siological,and -omics assays of guard cell function,plant signal transduction[M]. New York: Springer Press,2016.
[18] 赵书涛,武晓东,王策,等. 流式细胞仪的原理、应用及最新进展[J]. 现代生物医学进展,2011,11(22):4378-4381.
[19] 王继玥,余庭跃,张采波. 玉米转录组学研究进展[J]. 华北农学报,2014(S1):10-15.
[20] Leonhardt N,Kwak J M,Robert N,et al.Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive ABA hypersensitive protein phosphatase 2C mutant[J]. Plant Cell,2004,16(3):596-615.
[21] Wang R S,Pandey S,Li S,et al.Common and unique elements of the ABA-regulated transcriptome of Arabidopsis guard cells[J]. Bmc Genomics,2011,12(1):1-24.
[22] Zhao Z,Zhang W,Stanley B A,et al.Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways[J]. Plant Cell,2008,20(12):3210-3226.
[23] Zhu M,Zhu N,Song W Y,et al.Thiol-based redox proteins in abscisic acid and methyl jasmonate signaling in Brassica napus guard cells[J]. Plant Journal for Cell & and Molecula Biology,2014,78(3):491-515.
[24] Zhang T,Zhu M,Zhu N,et al.Identification of thioredoxin targets in guard cell enriched epidermal peels using cysTMT proteomics[J]. Journal of Proteomics,2015,133:48-53.
[25] Zhu M,Simons B,Zhu N,et al.Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging[J]. Journal of Proteomics,2009,73(4):790-805.
[26] Zhao Z,Stanley B A,Zhang W,et al.ABA-regulated G protein signaling in Arabidopsis guard cells: A proteomic perspective[J]. Journal of Proteome Research,2010,9(4):1637-1647.
[27] Zhu M,Dai S,Zhu N,et al.Methyl jasmonate responsive proteins in Brassica napus guard cells revealed by iTRAQ-based quantitative proteomics[J]. Journal of Proteom Research,2012,11(7):3728-3742.
[28] 王斯婷,李晓娜,王皎,等. 代谢组学及其分析技术[J]. 药物分析杂志,2010(9):1792-1799.
[29] Jin X,Wang R S,Zhu M,et al.Abscisic acid-responsive guard cell metabolomes of Arabidopsis wild-type and gpa1 G-protein mutants[J]. The Plant Cell,2013,25(12):4789-4811.
[30] Misra B B,Armas E D,Tong Z,et al.Metabolomic responses of guard cells and mesophyll cells to bicarbonate[J]. Plos One,2015,10(12):e0144206.
[31] Geng S,Misra B B,Armas E D,et al.Jasmonate-mediated stomatal closure under elevated CO₂ revealed by time-resolved metabolomics[J]. Plant Journal,2016,88(6):947-962.
[32] Schroeder J I,Allen G J,Hugouvieux V,et al.Guard cell signal transduction[J]. Annual Review of Plant Biology,2001,52(4):627-658.
[33] Casson S A,Hetherington A M.Environmental regulation of stomatal development[J]. Current Opinion in Plant Biology,2010,13(1):90-95.
[34] Murata Y,Mori I C,Munemasa S.Diverse stomatal signaling and the signal integration mechanism[J]. Annual Review of Plant Biology,2015,66(66):369.
[35] 刘婧,王宝山,谢先芝. 植物气孔发育及其调控研究[J]. 遗传,2011,33(2):131-137.